Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani

Góes, Larissa Brandão; Costa, Ana Bolena Lima da; Freire, Laurineide Lopes de Carvalho; Oliveira, Neiva Tinti de

doi:10.1590/s1516-89132002000200005

Cited by 24 publications

(17 citation statements)

References 21 publications

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“…Randomly amplified polymorphic DNA technique is valuable to distinguish the isolates of Trichoderma according to their species with the help of specific primers. The results are in agreement with the earlier workers (Zimand et al., 1994; Arisan‐Atac et al., 1995; Goes et al., 2002). They also distinguished the strain/species of Trichoderma through RAPD analysis.…”

Section: Discussionsupporting

confidence: 94%

“…Arisan‐Atac et al. (1995) and Goes et al. (2002) working with RAPD of species of Trichoderma also obtained this type of contradictory results.…”

Section: Discussionmentioning

confidence: 98%

“…The filamentous fungi, Trichoderma have attracted the attention because of their multiprong action against various plant pathogens (Harman et al., 2004). Attempts were made to characterize the variability among the isolates of Trichoderma species through molecular techniques (Fujimori and Okuda, 1993; Zimand et al., 1994; Arisan‐Atac et al., 1995; Turoczi et al., 1996; Gomez et al., 1997; Goes et al., 2002) and some of them were found suitable for strain identification and fast differentiation of strains. Some of the isolates included in the present study were found potential against several soilborne pathogens including Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

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Randomly Amplified Polymorphic DNA Markers for Trichoderma species and Antagonism Against Fusarium oxysporum f. sp. ciceris Causing Chickpea Wilt

Dubey¹,

Suresh²

2006

Journal of Phytopathology

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Randomly amplified polymorphic DNA (RAPD) was used to examine the genetic variability among 10 isolates of Trichoderma representing three species and their ability to antagonize Fusarium oxysporum f. sp. ciceris causing chickpea wilt using dual culture assay and volatile and non-volatile compound production. Using 10 oligonucleotide primers, we are able to distinguish among the species of Trichoderma. Primer OPA 13 efficiently differentiated all the isolates of Trichoderma in to their species except T 2 of Trichoderma viride. Some of the other primer as Primer OPA 10 also showed species-specific banding pattern. These species of Trichoderma were evaluated against the pathogen. All the isolates of the fungal antagonists caused significant reduction in the mycelial growth of the pathogen in vitro. The principle mechanism of mycoparasitism was observed as coiling of antagonistic hyphae around the pathogen hyphae and lysis.

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Section: Discussionsupporting

confidence: 94%

“…Arisan‐Atac et al. (1995) and Goes et al. (2002) working with RAPD of species of Trichoderma also obtained this type of contradictory results.…”

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Randomly Amplified Polymorphic DNA Markers for Trichoderma species and Antagonism Against Fusarium oxysporum f. sp. ciceris Causing Chickpea Wilt

Dubey¹,

Suresh²

2006

Journal of Phytopathology

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“…(Arisan-Atac et al, 1995; Goes et al, 2002; Muthumeenakshi and Mills, 1995). RAPD markers were used to estimate the genetic variation within 30 T. asperellum isolates.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Novel Trichoderma asperellum Isolates to Select Effective Biocontrol Agents Against Tomato Fusarium Wilt

El_Komy

Saleh

Eranthodi

et al. 2015

The Plant Pathology Journal

163

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The use of novel isolates of Trichoderma with efficient antagonistic capacity against Fusarium oxysporum f. sp. lycopersici (FOL) is a promising alternative strategy to pesticides for tomato wilt management. We evaluated the antagonistic activity of 30 isolates of T. asperellum against 4 different isolates of FOL. The production of extracellular cell wall degrading enzymes of the antagonistic isolates was also measured. The random amplified polymorphic DNA (RAPD) method was applied to assess the genetic variability among the T. asperellum isolates. All of the T. asperellum isolates significantly reduced the mycelial growth of FOL isolates but the amount of growth reduction varied significantly as well. There was a correlation between the antagonistic capacity of T. asperellum isolates towards FOL and their lytic enzyme production. Isolates showing high levels of chitinase and β-1,3-glucanase activities strongly inhibited the growth of FOL isolates. RAPD analysis showed a high level of genetic variation among T. asperellum isolates. The UPGMA dendrogram revealed that T. asperellum isolates could not be grouped by their anta- gonistic behavior or lytic enzymes production. Six isolates of T. asperellum were highly antagonistic towards FOL and potentially could be used in commercial agriculture to control tomato wilt. Our results are consistent with the conclusion that understanding the genetic variation within Trichoderma isolates and their biochemical capabilities are required for the selection of effective indigenous fungal strains for the use as biocontrol agents.

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“…The antagonism of Trichoderma isolates against S. rolfsii was examined as described by Dennis and Webster [1971] and Goes et al [2002]. The fungal isolates were cultivated in Petri dishes with 20 mL of PDA for 7 days.…”

Section: In Vitro Antagonism Between Trichoderma and S Rolfsiimentioning

confidence: 99%

Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists

et al. 2017

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The fungus Trichoderma is a teleomorph of the Hypocrea genus and associated with biological control of plant diseases. The microscopic, biochemical, and molecular characterization of Trichoderma was carried out and evaluated for in vitro antagonistic activity against the fungal pathogen Sclerotium rolfsii causing stem rot disease in groundnut. In total, 11 isolates of Trichoderma were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11, T. virens NBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed by T. koningii MTCC 796 (67.03%), T. viride NBAII Tv23 (63.74%), and T. harzianum NBAII Th1 (60.44%). Strong mycoparasitism was observed in the best antagonist Tvs12 strain during 6-12 DAI. The specific activity of cell wall-degrading enzymes - chitinase and β-1,3-glucanase - was positively correlated with growth inhibition of the test pathogen. In total, 18 simple sequence repeat (SSR) polymorphisms were reported to amplify 202 alleles across 11 Trichoderma isolates. The average polymorphism information content for SSR markers was found to be 0.80. The best antagonist Tvs 12 was identified with 7 unique SSR alleles amplified by 5 SSR markers. Clustering patterns of 11 Trichoderma strains showed the best antagonist T. virens NBAII Tvs 12 outgrouped with a minimum 3% similarity from the rest of Trichoderma.

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Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani

Abstract: Random Amplified Polymorphic DNA (RAPD) procedure was used to examine the genetic variability among fourteen isolates of

Cited by 24 publications

References 21 publications

Randomly Amplified Polymorphic DNA Markers for Trichoderma species and Antagonism Against Fusarium oxysporum f. sp. ciceris Causing Chickpea Wilt

Randomly Amplified Polymorphic DNA Markers for Trichoderma species and Antagonism Against Fusarium oxysporum f. sp. ciceris Causing Chickpea Wilt

Characterization of Novel Trichoderma asperellum Isolates to Select Effective Biocontrol Agents Against Tomato Fusarium Wilt

Antipathy of <b><i>Trichoderma</i></b> against <b><i>Sclerotium rolfsii </i></b>Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists

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