RAPD and Cytogenetic Study in F1 and F2 of Interspecific Cross between Gossypium arboreum*Gossypium anomalum

Sushir, K. V.; Mehetre, S. S.; Patil, Sudam C.; Kamdi, Sandip R.

doi:10.1508/cytologia.73.213

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Chromosomal crossing over during meiosis and loss of primer attachment pair sites could be another reason for producing novel allelic patterns in genotypes (Sheidai et al, 2008). This phenomenon has also been reported in other plant species hybrids (Smith et al, 1996;Sushir et al, 2008).…”

Section: Discussionsupporting

confidence: 56%

Methodology Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Noormohammadi¹,

Y²,

Sheidai³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F 2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers produced 261 reproducible bands, with an average of 4.18 bands per primer and 22% polymorphism. The OPB12/OPH08 primer gave the highest effective number of alleles (N E ), and the largest Shannon index (I), Nei's genetic diversity (H), and polymorphism information content (PIC) values. Some RAPD bands were present in the parental genotypes but were absent in their hybrids. Ten ISSR primers produced 206 reproducible bands, with 49.4% polymorphism. The UBC807 locus gave the highest N E , I, H, and PIC values. Some ISSR bands occurred only in the parental genotype, while others were Genetic variation of intraspecific cotton hybrids only present in the hybrid genotypes. Four microsatellite loci produced 12 alleles, ranging from 181 to 236 bp, with 54% polymorphism. The TMB1421 locus, with a monomorphic allele, was digested with three restriction enzymes (CAP-microsatellite) to evaluate sequence variations among samples. Association analysis between molecular markers and agronomic data revealed a significant correlation between ISSR-UBC807-1500 and yield. The Mantel test performed among the genetic distance matrices obtained from RAPD, ISSR and SSR showed a nonsignificant regression between RAPD versus ISSR and ISSR versus SSR, while RAPD versus SSR showed a significant regression; regression for ISSR and RAPD+ISSR+SSR combined data was also significant. Cluster analysis (UPGMA) based on these three types of molecular markers differentiated cotton genotypes and their progenies. Among the molecular markers, ISSR revealed more genetic variation among the genotypes. However, using all three types of molecular markers provided a better overall view of cotton genome polymorphism.

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Section: Discussionsupporting

confidence: 56%

Methodology Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Noormohammadi¹,

Y²,

Sheidai³

et al. 2013

Genet. Mol. Res.

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“…Dendrogram resulting from UPGMA cluster and POC plot based on RAPD data from control, 1 mT for 1 h, 7 mT for 3 h in canola. or rearrangement of some nucleotides (Sushir et al 2008). Our previous work showed a significant difference of meiotic characters in EMF pretreated plants as compared to control.…”

Section: Discussionmentioning

confidence: 73%

Induction of genetic variation by electromagnetic fields inZea maysL. andBrassica napusL.

et al. 2015

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Effect of electromagnetic fields (EMF) on some growth parameters, protein content and genetic variations were investigated in early vegetative growth of Brassica napus L. (canola) and Zea mays L. (maize). The seeds of both plants were exposed to EMF treatments. Maize seeds were treated with 0, 3 and 10 mT for 4 h exposure time, and canola seeds were treated with 0, 1 mT for 1 h and 7 mT for 3 h, respectively. Results showed that seed germination increased significantly in maize, and decreased in canola seeds. Root length, shoot length, fresh weigh and dry weight increased significantly in maize and canola as compared to control. Total protein content in maize significantly increased at 3 mT and then decreased in 10 mT for 4 h, but in canola increased protein content under EMF treatments. RAPD analysis showed 104 total bands of 14 primers in maize, out of which 72 bands (about 69.2%) were polymorphic bands, and 65 polymorphic bands (51.6%) in canola were determined from a total of 126 bands. Primers showing maximum number of polymorphic bands were OPM-11 and OPI-07 in maize and OPA-10 in canola. It was found that seed pretreatment to low density of EMF could be used for improving growth and genetic variations in maize and canola plants.

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“…Chromosomal crossing over during meiosis may result in loss of primer attachment pair sites in the offspring leading to novel ISSR pattern in the offspring (Smith et al, 1996). Sushir et al (2008) carried out cytogenetic and RAPD analysis of F 1 and F 2 progenies of the interspecific cross between Gossypium arboretum X G. anomalum and reported that among nine F 2 segregates, F 2 to 1 progeny plants showed one additional band than F 1 and F 2 to 5 progeny plant showed the recombination event. On the contrary in plants F 2 to 6 and F 2 to 8 loss of priming sites happened showing that recombination between A and B genomes of G. arboreum and G. anomalum respectively is possible (Sushir et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Sushir et al (2008) carried out cytogenetic and RAPD analysis of F 1 and F 2 progenies of the interspecific cross between Gossypium arboretum X G. anomalum and reported that among nine F 2 segregates, F 2 to 1 progeny plants showed one additional band than F 1 and F 2 to 5 progeny plant showed the recombination event. On the contrary in plants F 2 to 6 and F 2 to 8 loss of priming sites happened showing that recombination between A and B genomes of G. arboreum and G. anomalum respectively is possible (Sushir et al, 2008). Sheidai et al (2008) also used RAPD markers to analyse different cotton genotypes and reported the occurrence of novel RAPD bands in the hybrids not observed in their parents and also reported the absence of RAPD bands in the hybrids which were present in their parents.…”

Section: Discussionmentioning

confidence: 99%

Simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) analyses of genetic diversity in tissue culture regenerated plants of cotton

Sheidai¹

2012

Afr. J. Biotechnol.

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Cotton is one of the main economic crop plants of Iran cultivated under continuous artificial selectionand cultivation which may lead to genetic erosion and possible loss of useful genetic loci resulting in vulnerability to pests and diseases. For this reason increasing and improving the amount of genetic diversity in cotton germplasm through tissue culture is important. The present report considers genetic diversity induced in tissue culture regenerated plants of three cotton cultivars namely Mehr, Sindose and their hybrid Mehr X Sindose. Surface of seeds were disinfected with 70% ethanol for 2 min and then treated with 5% hypochlorite solution for 20 min. Finally, they were washed 3 to 4 times with sterile distilled water and inoculated aseptically on Murashige and Skoog (MS) basal medium free hormones. Single nodes resulted from seedlings cultured as explants. Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) primers used produced different number of bands in the genotypes studied showing different levels of molecular polymorphisms in each cultivar. Some common and few specific ISSR/SSR loci were indentified while some bands were present in all the genotypes except one indicating genetic changes in them. Analysis of molecular variance (AMOVA) test showed significant difference (p < 0.05) for ISSR markers but not for SSR markers. Molecular trees obtained showed genetic variations among the regenerated plants of each cultivar due to tissue culture.

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RAPD and Cytogenetic Study in F1 and F2 of Interspecific Cross between Gossypium arboreum*Gossypium anomalum

Cited by 5 publications

References 13 publications

Methodology Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Methodology Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Induction of genetic variation by electromagnetic fields inZea maysL. andBrassica napusL.

Simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) analyses of genetic diversity in tissue culture regenerated plants of cotton

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