RAPD and ISSR analyses of Saccharomyces cerevisiae isolates from different sources

Hammadi, Sawas Younus; Hussein, A. S.; Majeed, Duha Mysire; Dheeb, Batol Imran; Ismail, Eman Noaman

doi:10.24126/jobrc.2018.12.2.537

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…Thyroid preservation can be applied in some cases, when the cancer is less aggressive (differentiated cancer, no evidence of lymph node proliferation, i.e., major genetic modifications, p53 mutations etc.) in patients under the age of 45 years (Hammadi et al, 2019). An operation should be performed if the diagnosis is established to be differentiated thyroid cancer (such as papillary thyroid cancer) or if a needle biopsy is suspected, while a watchful wait strategy is not recommended in any evidence-based guidelines.…”

Section: Goitermentioning

confidence: 99%

Thyroid Hormone Concentration and Receptor

Ali

Majeed²

2022

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Hormones are hormones secreted by the thyroid gland and their primary function is to regulate the speed of metabolism in many tissues. The thyroid gland releases its hormones into the bloodstream in response to a regulating hormone released by the pituitary gland called thyroidstimulating hormone. It is the only organ in the body that has the ability to synthesize iodine in large quantities for use in the synthesis of thyroid hormones. It consists of two lobes, joined together by the isthmus, located above the windpipe, just below the cartilage, which is the marker for its location. The weight of the thyroid glands in humans ranges from 15-to 20 grams and the blood supply changes depending on the functioning of the gland. There are lymphatic vessels, but their function in relation to endocrine function is uncertain.

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Section: Goitermentioning

confidence: 99%

Thyroid Hormone Concentration and Receptor

Ali

Majeed²

2022

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Production and characterization of bioemulsifier from local isolate of Saccharomyces cerevisiae strain JZT351: Antioxidant and antimicrobial effects

Thraeib,

Altemimi,

Al‐Manhel

et al. 2024

eFood

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The widespread use and circulation of industrial emulsifiers pose significant health risks, compounded by their limited availability and high cost. Consequently, there is growing interest in exploring the potential of natural sources, such as microorganisms like yeast, for emulsifier production. In this study, 25 strains of Saccharomyces cerevisiae were isolated from 17 distinct local sources. The yeasts were characterized based on cell shape, size, and colony morphology using yeast malt agar, followed by morphological, microscopic, and biochemical analyzes. Among the isolates, AC1 from a vinegar starter demonstrated superior bioemulsifier production. Emulsification efficiency tests revealed that AC1 exhibited values of 7.1 cm, 0.71 nm, and 34.50%, outperforming other isolates. The biomass yield was approximately 4.35 g/L. This strain was registered in the gene bank as JZT351 (OR115510) after a 100% match with S. cerevisiae. Optimal bioemulsifier production conditions for JZT351 were identified using a liquid yeast extract peptone dextrose medium, with date juice replacing 75% of the glucose, at pH 5.5, 30°C, and 72 h. The resulting emulsification activity, index, and oil displacement were 8.69 cm, 0.95 nm, and 39.7%, respectively. Fourier‐transform infrared spectroscopy (FTIR) compared the functional groups of the bioemulsifier with those of conventional emulsifiers. Molecular characterization was confirmed by 1H NMR. FTIR spectra revealed bioemulsifier bands at 3443.28, 2929.34, 1656.559, 1534.09, 807.06, and 1656.55 cm−1. At 0.5–5 ppm, signals and spectral frequencies corresponded to a mannoprotein structure. The bioemulsifier exhibited 58.09% antioxidant activity at 10 mg/mL, as assessed by DPPH scavenging. It showed the highest inhibition against Pseudomonas aeruginosa and the least against Candida albicans.

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