RAPD e ITS Detectan Variación Molecular en Poblaciones Chilenea de Beauveria bassiana

Becerra, Viviana; C, Mario Paredes; M, Carmen Rojo

doi:10.4067/s0365-28072007000200001

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…B . bassiana genetic diversity has been determined via analysis of ITS-rDNA sequences [ 38 ]). Using the ITS rDNA region [ 39 , 40 ] evaluated the genetic diversity and phylogeny of five strains of B .…”

Section: Discussionmentioning

confidence: 99%

“Metabolomic diversity of local strains of Beauveria bassiana (Balsamo) Vuillemin and their efficacy against the cassava mite, Tetranychus truncatus Ehara (Acari: Tetranychidae)”

et al. 2022

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A desirable substitute for chemical pesticides is mycopesticides. In the current investigation, rDNA-ITS (Internal transcribed spacer) and TEF (Transcriptional Elongation Factor) sequencing were used for molecular identification of six Beauveria bassiana strains. Both, leaf discs and potted plant bioassaye were carried out to study their pathogenicity against the cassava mite, Tetranychus truncatus. LC50 and LC90 values of potential B. bassiana strains were estimated. We also discovered a correlation between intraspecific B. bassiana strains pathogenicity and comprehensive metabolome profiles. Bb5, Bb6, Bb8, Bb12, Bb15, and Bb21 strains were identified as B. bassiana by sequencing of rDNA-ITS and TEF segments and sequence comparison to NCBI (National Center for Biotechnology Information) GenBank. Out of the six strains tested for pathogenicity, Bb6, Bb12, and Bb15 strains outperformed against T. truncatus with LC50 values 1.4×106, 1.7×106, and 1.4×106 and with a LC90 values 7.3×107, 1.4×108, and 4.2×108 conidia/ml, respectively, at 3 days after inoculation and were considered as potential strains for effective mite control. Later, Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the above six B. bassiana strains was done on secondary metabolites extracted with ethyl acetate revealed that the potential B. bassiana strains (Bb6, Bb12, and Bb15) have higher levels of acaricidal such as Bis(dimethylethyl)-phenol: Bb6 (5.79%), Bb12 (6.15%), and Bb15 (4.69%). Besides, insecticidal (n-Hexadecanoic acid), and insect innate immunity overcoming compound (Nonadecene) were also identified; therefore, the synergistic effect of these compounds might lead toa higher pathogenicity of B. bassiana against T. truncatus. Further, these compounds also exhibited two clusters, which separate the potential and non-potential strains in the dendrogram of Thin Layer Chromatography. These results clearly demonstrated the potentiality of the B. bassiana strains against T. truncatus due to the occurrence of their bioactive volatile metabolome.

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Section: Discussionmentioning

confidence: 99%

“Metabolomic diversity of local strains of Beauveria bassiana (Balsamo) Vuillemin and their efficacy against the cassava mite, Tetranychus truncatus Ehara (Acari: Tetranychidae)”

et al. 2022

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“…Alignments and phylogenetic analyses confirmed the B. bassiana and M. anisopliae strains taxonomic identity. Analysis of ITS-rDNA sequences have been applied to determine the genetic diversity of M. anisopliae and B. bassiana (Wall et al, 2005;Entz et al, 2005;Becerra et al, 2007;Carneiro et al, 2008;Freed et al, 2011). Thus, Bautista-Galvez et al 2012, made the genetic characterization of M. anisopliae strains obtaining fragments of 600 to 800 bp by PCR amplification from the ITS1-ITS4 rDNA regions.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae

Mora¹,

ChacÃ3n-Orozco²,

Harakava³

et al. 2016

Afr. J. Microbiol. Res.

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Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations: 1×10 6 , 1×10 7 , 1×10 8 and 1×10 9 conidia.ml-1 were in vitro evaluated and the lethal concentrations (LC 50 and LC 90) as lethal time (LT 50 and LT 90) were determined. Sequencing of rDNA-ITS 500 bp fragments allowed the identification of Ma10MI, Ma12MI, Ma58MI and Ma11MI isolates as M. anisopliae and Bb11MI, Bb79MI, Bb53MI and Bb27MI isolates as B. bassiana by sequence comparison to GenBank. According to the pathogenicity test, B. bassiana strains with higher performance for G. mellonella were Bb53MI and Bb79MI with LC 50 = 3.98×10 7 and 1.04×10 7 conidia.ml-1 and LT 50 = 5.46 and 5.27 days, respectively, as well as to T. molitor was Bb79MI with LC 50 = 1.03×10 7 conidia.ml-1 and LT 50 = 5.57 days. The M. anisopliae strains with the best performance were Ma58MI and Ma10MI both T. molitor (LC 50 = 1.0×10 6 and 1.0×10 7 conidia.ml-1 and LT 50 = 4.06 and 5.22 days, respectively) and G. mellonella (LC 50 = 6.3×10 7 and 1.1×10 8 conidia.ml-1 and LT 50 = 5.18 and 6.37 days, respectively), these isolates might be considered as new promising candidates for the microbial pest control.

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“…Currently, molecular biology techniques are used to identify and characterize the genetic variability of entomopathogenic fungi. The most precise way to determine the levels of diversity and intraspecific genetic structure is through the use of molecular markers (Becerra et al, 2007). Molecular tools are being widely used for the recognition of fungal species, the ITS region of the rDNA is considered the official DNA barcode for fungi since it has hypervariable sequences that allow recognition both at the species level and at lower levels (Schoch et al, 2012;Toju et al, 2012).…”

Section: Molecular Identification Of Entomopathogenic Fungimentioning

confidence: 99%

Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

et al. 2021

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RAPD e ITS Detectan Variación Molecular en Poblaciones Chilenea de Beauveria bassiana

Cited by 6 publications

References 13 publications

“Metabolomic diversity of local strains of Beauveria bassiana (Balsamo) Vuillemin and their efficacy against the cassava mite, Tetranychus truncatus Ehara (Acari: Tetranychidae)”

“Metabolomic diversity of local strains of Beauveria bassiana (Balsamo) Vuillemin and their efficacy against the cassava mite, Tetranychus truncatus Ehara (Acari: Tetranychidae)”

Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae

Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

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