Rapid (24h) neutralization assay for the detection of antibodies to human cytomegalovirus using a monoclonal antibody to an HCMV early nuclear protein

Braun, W.; C, Schacherer

doi:10.1016/0166-0934(88)90085-7

Cited by 12 publications

(3 citation statements)

References 21 publications

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“…Undifferentiated cells of either type were unable to express the HCMV major IE antigen [13,20]. On the other hand, in several human malignant brain-derived cell lines, whose HCMV infection was restricted to the expression of IE antigen, differentiation agents failed to induce HCMV replication [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. Rhabdomyosarcoma cell lines previously used for HCMV infection showed either low sensitivity [25] or were nonpermissive for HCMV [6].…”

Section: G G C a A G T T C T G C A A T G C C G C -3 ' ) And Cmv19 ( 5mentioning

confidence: 99%

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Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells

Činátl

Radsak

Rabenau

et al. 1994

Archives of Virology

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The replication of human cytomegalovirus (HCMV) was investigated in a new human rhabdomyosarcoma cell line (KFR) with morphological and biochemical characteristics of fetal striated muscle precursors (rhabdomyoblasts). KFR cells exhibited the unique property for spontaneous morphological transformation from a poorly-differentiated state into well-differentiated (myotube-like) rhabdomyoblasts. The poorly-differentiated rhabdomyoblasts promoted both complete viral gene expression and the production of infectious virus. In contrast, in well-differentiated rhabdomyoblasts HCMV infection was abortive. The results showed that replication of HCMV in this human rhabdomyosarcoma cell line depended on the state of cellular differentiation.

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Section: G G C a A G T T C T G C A A T G C C G C -3 ' ) And Cmv19 ( 5mentioning

confidence: 99%

“…For viral antigen detection immunoperoxidase staining of the cells was performed at 24 and 72h post infection (p.i.) using monoclonal antibodies (DuPont) directed against a 72-kDa immediate-early (IE) or a 67-kDa late (L) HCMV protein, respectively, as described previously [3]. HCMV DNA of replicated virus was as described elsewhere [24].…”

mentioning

confidence: 99%

Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells

Činátl

Radsak

Rabenau

et al. 1994

Archives of Virology

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“…It has been shown that neutralizing antibodies and IgG-class antibody detected by enzyme-linked immunosorbent assay (ELISA) are not correlated to each other [9,57]. AIDS patients and immunocompromised renal transplant recipients show higher ELISA antibody titers but lower neutralizing antibody titers than healthy seropositive controls [8,18].…”

Section: Neutralizing Antibody Response To Hcmvmentioning

confidence: 99%

Humoral immune response to human cytomegalovirus infection: diagnostic potential of immunoglobulin class and IgG subclass antibody response to human cytomegalovirus early and late antigens

Weber¹,

Braun²,

Cinatl³

et al. 1993

Clin Investig

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For the development of effective prophylaxis (hyperimmune globulins) and improvement of serological testing for human cytomegalovirus (HCMV) infection in immunocompromised patients it is essential to characterize the viral encoded proteins and the humoral immune response in terms of neutralizing antibodies and immunoglobulin class and IgG subclass reactivity to "early" and "late" HCMV proteins. The major neutralizing epitopes have been identified and screening of donor sera for neutralizing antibody by either conventional neutralization assays or enzyme-linked immunosorbent assay using recombinant antigens may help to improve the efficacy of hyperimmune globulin prophylaxis. The humoral response to individual HCMV proteins has been thoroughly investigated in immunocompromised patients. Antibodies against HCMV induced "early" antigens are not exclusively associated with active infection but may indicate an elevated risk for cytomegalic inclusion disease in immunocompromised patients. With a sensitive western blot technique. IgM and IgA antibodies against HCMV "late" proteins can be detected in sera from healthy seropositive individuals. Serum samples from subjects suffering from cytomegalic inclusion disease show significantly larger broader immune responses compared with healthy HCMV antibody carriers. Promising results using recombinant antigens corresponding to immunodominant epitopes for the detection of HCMV specific antibodies have been published.

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Detection of neutralizing antibodies against human cytomegalovirus: Influence of strain variation

Weber

Klinghardt

Lux

et al. 1993

Journal of Medical Virology

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The influence of human cytomegalovirus (HCMV) strain variation on neutralizing antibody titers was investigated in sequential sera obtained from 12 organ transplant recipients (11 renal transplant recipients, 1 liver transplant patient) suffering from primary or secondary HCMV infection. Cross-neutralization assays using either the international HCMV reference strain AD169 or individual clinical isolates from patients showed congruent results. Restriction enzyme analysis of the hypervariable alpha-sequence of the L-S junction of AD169 and HCMV isolates amplified by polymerase chain reaction (PCR) were carried out to confirm the expected strain differences. At least 6 groups of clinical strains were differentiated. The results of this study demonstrated that despite the strain heterogenicity of HCMV, the neutralization assay using AD169 permitted a reliable and quantitative serologic detection of neutralizing antibodies against HCMV.

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Rapid (24h) neutralization assay for the detection of antibodies to human cytomegalovirus using a monoclonal antibody to an HCMV early nuclear protein

Cited by 12 publications

References 21 publications

Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells

Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells

Humoral immune response to human cytomegalovirus infection: diagnostic potential of immunoglobulin class and IgG subclass antibody response to human cytomegalovirus early and late antigens

Detection of neutralizing antibodies against human cytomegalovirus: Influence of strain variation

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