Rapid Analysis of Cell–Nanoparticle Interactions using Single‐Cell Raman Trapping Microscopy

Abstract

Iron oxide nanoparticles have been used in preclinical studies to label stem cells for non-invasive tracking and homing.The search continues for novel particle candidates that are suitable for clinical applications.Since standardanalyses to investigate cell-particle interactions and safety are laborintensive,a ne fficient procedure is required to guide future particle development and to exclude adverse health effects.The application of combined Raman trapping microscopyw ith fluidic chips is reported for the analysis of single cells labeled with different types of aminated iron oxide particles.M ultivariate data analysis revealed Raman signal differences that could be clearly assigned to cell-particle interactions and cytotoxicity,r espectively.Avalidation dataset verified that more than 95 %o ft he spectra were correctly classified. Thus, our approach enables rapid discrimination of non-hazardous from cytotoxic nanoparticles as aprerequisite for safe clinical applications.