Rapid and high yield Extraction method for Saponins from Safed musli

Deore, S. L.; Baviskar, Bhushan A; Rangari, Ashwini Suresh

doi:10.5530/pj.2015.4.1

Cited by 11 publications

(8 citation statements)

References 9 publications

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“…First, soyasaponin was extracted from soybean seeds by the microwave-assisted solvent extraction method. Successful extraction of soyasaponin by this method was also reported by Deore et al., (2015) . Waziiroh and Kamilia (2018) also suggested this solvent extraction as a reliable method for soyasaponin extraction from plant sources.…”

Section: Discussionmentioning

confidence: 58%

Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin

et al. 2020

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The present study aimed to evaluate the immunopotentiating effect of plant-derived soyasaponin and its immunogenicity in chickens challenged with Newcastle disease virus ( NDV ). Soyasaponin was extracted from soybean seeds and detected using the phytochemical tests, followed by quantification through the dry-weight method. One-day-old broiler chicks (n = 90) were divided into 3 groups, named as A, B, and C. Group A birds were orally administrated with soyasaponin (5 mg/kg), followed by immunization with inactivated ND vaccine intramuscularly ( IM ), whereas group B birds were vaccinated with inactivated ND vaccine alone. Group C birds were kept unvaccinated. A booster dose on day 21 was also administered IM to group A and B birds. At day 35, all 3 groups were challenged with NDV. To determine the immunogenicity potential of soyasaponin, antibody titer was measured using the hemagglutination inhibition test before and after the NDV challenge. Histochemical examination was performed to determine the pathological changes associated with NDV infection. Foam formation and hemolytic activity confirmed the presence of saponin in soya bean extract. Group A birds showed a higher antibody response compared with group B and C birds. The disease challenge study showed that soyasaponin-adjuvanted NDV vaccine provided complete protection to group A birds against ND. Moreover, no side effects of soyasaponin were observed on the growth performance of birds during the experiment. Therefore, we can conclude that soyasaponin is a potential immunogenic agent and therefore could be a promising candidate to launch a protective humoral response against ND in chickens.

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Section: Discussionmentioning

confidence: 58%

Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin

et al. 2020

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“…The propolis extraction requires special techniques because of its unique characteristics. This study used ultrasound-assisted extraction (UAE) because its wave increases the solubility of active compounds through a mechanical effect to break the matrix, thus, producing highquality extract (Dhanani et al, 2013;Deore & Baviskar, 2015). Similarly, Khacha-Ananda et al, (2013) also used UAE and the yielded propolis extraction of 15.66%.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity and Total Phenolic Content of Stingless Bee Propolis from Indonesia

Fikri

Sulaeman

Marliyati

et al. 2019

Journal of Apicultural Science

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The aim of this study was to investigate antioxidant activity, total phenolic compounds and flavonoids of propolis from three provinces of Indonesia with two methods of extraction. Stingless bee propolis from Banten, South Kalimantan, and South Sulawesi was prepared through ultrasound-assisted extraction with water and ethanol solvents, separately. Antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid contents were analyzed by Folin-Ciocalteu colorimetric and aluminum chloride (AlCl3) methods, respectively. The result showed that the antioxidant activity expressed as IC50 of Indonesian propolis ranged from 452.52 to 1027.29 mg/L. Ethanol extracts showed higher antioxidant activity than water extracts. Total phenolic and flavonoids content detected in the samples ranged from 10.00 to 28.65 mg/g GAE and 0.76 to 3.39 mg/g QE, respectively. The ethanol extract of propolis from South Kalimantan had the highest total phenolic contents, while that from South Sulawesi possessed the highest total flavonoids.

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“…The wave increases cell permeability thus result in optimum solubility of the active component. 15 The procedures refered to Trusheva method with slight modification. 16 Raw propolis was added to solvents (1:10) then treated with ultrasound for 4h.…”

Section: Preparation Of Extractmentioning

confidence: 99%

Antiemetic Activity of Trigona spp. Propolis from Three Provinces of Indonesia with Two Methods of Extraction

Fikri¹,

Sulaeman²,

Marliyati³

et al. 2017

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Introduction:Propolis is a resinous substance collected by bees to protect their hives. One of the least explored function of propolis is its antiemetic activity. The present study aimed to investigate the antiemetic activity of Trigona spp. propolis from three provinces of Indonesia. Methods: Ultrasound-assisted extraction using two kinds of solvent, water and ethanol was conducted to extract propolis from South Sulawesi, South Kalimantan and Banten. Antiemetic activity was observed on copper sulfate-induced emesis in young chicks. Result: All propolis indicated antiemetic activity, in which water extract had higher activity than ethanol extract. Regardless the type of solvents, propolis from South Sulawesi showed higher antiemetic activity than others. Conclusion: This study demonstrated that propolis has antiemetic activity, in which propolis from South Sulawesi showed the highest activity and water extraction is recommended. Key words: Antiemetic, Propolis, Retching, Stingless bee, Trigona spp. factor that influences propolis biological activity. However, there are conflicting result on types of solvent used to generate highest biological activity. Some studies found ethanol extract had higher antioxidant activity and total phenol compared to water extract. While, some other found water is a better solvent to generate high antioxidant activity and total phenol. 11,12,13 This study aimed to investigate the antiemetic activity of propolis from three provinces of Indonesia, using two types of solvent (ethanol and water). We applied ultrasound-assisted extraction to yield more efficient and effective extract. Al Mukhlas 14 MATERIALS AND METHOD MaterialsRaw propolis were collected by bee-keeper in South Sulawesi, South Kalimantan and Banten. The species of bees were all stingless bees (Trigona spp.) namely Tetragonula biroi(South Kalimantan), HeteroTrigona itama (South Kalimantan) and Tetragonula laevicep (Banten). We used copper sulfate (CuSO 4 ) as emetogenic substance and metoclopramide as positive control. Dimethyl sulfoxide (DMSO) and Tween 80 were used as solvent for injected propolis. AnimalsThe young chicks (aged 4 days) and weighing from 50-80 g were purchased from Cibadak Indah Sari Farm, Jakarta, Indonesia. All animal experiments were carried out in accordance with the acts of The 121 found water extract of Brazilian propolis demonstrated higher antiemetic activity than hydro alcohol extract. The reason is still unclear. However, it is suspected that there are other water soluble compounds, such as glucoside, polysaccharides, saponins that work as inhibitor of some emesis receptors. 22Some experimental animals have been widely developed as an emetic model such as: ferret, mink, monkey, pig, rat, frog, dog and chick. 23 Chick as emesis animal model has been used by many researchers. 7,17,24,25,26 These model is better than frog emesis model because it has some advantages such as, easy to handle and able to visually observed and required shorter time. 26CuSO 4 -induced chicks emesis model are su...

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Rapid and high yield Extraction method for Saponins from Safed musli

Cited by 11 publications

References 9 publications

Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin

Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin

Antioxidant Activity and Total Phenolic Content of Stingless Bee Propolis from Indonesia

Antiemetic Activity of Trigona spp. Propolis from Three Provinces of Indonesia with Two Methods of Extraction

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