Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis

Zheng, Qi; Dessai, Ananta Porobo; Prakash, Channapatna S.

doi:10.1007/s002990050037

Cited by 8 publications

(9 citation statements)

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“…Somatic embryo development of sweet potato cultivar Kokei occurred by transferring proembryonic masses to medium containing 15.1 mM ABA (Otani and Shimada, 1996). Similar results were reported by Zheng et al (1996) showing that the addition of 9.5 mM of ABA resulted in 100% of the explants producing somatic embryos of sweet potato`PI318846-3'. In our work the number of somatic embryos decreased in medium with a high level of ABA (10 mM).…”

Section: Discussionsupporting

confidence: 86%

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Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Cantliffe

2001

In Vitro Cell.Dev.Biol.-Plant

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Somatic embryos of sweet potato have potential as synthetic seeds. The effects of abscisic acid (ABA) (0.0, 0.1, 1.0, 10.0 and 50.0 mM) were examined to improve synchrony and proliferation of somatic embryos. Transferring embryonic cultures at day 7 to solid medium with 0.1 or 1.0 mM ABA produced more torpedo and cotyledonary somatic embryos compared to those cultures transferred at day 0. The development of embryos in suspension culture supplemented with ABA was poor. However, when calli proliferation cultures were in gelled medium and pulsed with 0.1 mM ABA for 14 d, the number of somatic embryos increased. Proembryonic masses cultured in mannitol-containing medium Y 21X5 MPa increased embryo development and synchrony of embryo development. Thus, in this work ABA and mannitol have been shown to improve both the total number and the synchrony of sweet potato somatic embryos.

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Section: Discussionsupporting

confidence: 86%

“…However, ABA has been widely used to enhance somatic embryogenesis (Kokubu et al, 1993;Otani and Shimada, 1996;Zheng et al, 1996;Linossier et al, 1997). Somatic embryo development of sweet potato cultivar Kokei occurred by transferring proembryonic masses to medium containing 15.1 mM ABA (Otani and Shimada, 1996).…”

Section: Discussionmentioning

confidence: 99%

Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Cantliffe

2001

In Vitro Cell.Dev.Biol.-Plant

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“…Rodríguez and Wetzstein (1994) found that the type and concentration of auxin used had an effect on the somatic embryo morphology and their subsequent conversion into plantlets in Carya illinoinensis. Zheng et al (1996) observed that the use of ABA promoted the production of a large number of somatic embryos per explant and also increased the conversion of embryos into plantlets in sweet potato and Nickle and Yeung (1994) had similar observations in Daucus carota. These results, thus, indicate that, although depending upon the plant species under consideration, the hormonal requirements may vary, but the conditions favouring somatic embryo formation also favour their conversion into plants.…”

Section: Morphological Characteristicssupporting

confidence: 67%

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Park

Cho

Moon

et al. 2010

Plant Cell Tiss Organ Cult

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In an attempt to obtain insight into the differential responsiveness of different genotypes of Kalopanax septemlobus regarding their embryogenic capacity, several parameters such as endogenous hormonal levels, DNA content, embryogenic callus proliferation and somatic embryogenesis were studied in several genotypes of this plant. Also, to understand the effect of the age of the explants on their embryogenic capacity, the same parameters were studied in two embryogenic cell lines of different ages in the selected genotype. In the present study, it was observed that the cytokinins/abscisic acid (ABA) ratio plays an important role in embryogenic capacity in the studied genotypes of K. septemlobus species. A decrease in embryogenic capacity of callus was observed with increasing age, along with a marginal decrease in the DNA content of nuclei. Further, it can be suggested from our results that young embryogenic callus is a better choice for somatic embryo formation than the long-term-maintained callus in K. septemlobus.

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“…Although the mode of action of ABA during embryo development is not well understood (Hetherington and Quatrano 1991), ABA has been widely employed to synchronize somatic embryogenesis and to improve developmental attributes and conversion of somatic embryos (Nickle and Yeung 1994). The presence of ABA has also been found in other studies to be crucial for the maturation of sweet potato embryos (Zheng et al 1996;Otani and Shimada 1996;Triqui et al 2008).…”

Section: Discussionmentioning

confidence: 94%

Transgenic sweet potato expressing mammalian cytochrome P450

Anwar

Watanabe

2010

Plant Cell Tiss Organ Cult

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Sweet potato [Ipomoea batatas (L.) Lam] is considered to be recalcitrant to transformation and regeneration because of its genotype-dependent in vitro responses. The lack of a genotype-independent transformation and regeneration system limits biotechnological applications in this plant species. To establish a transformation system for a diverse group of sweet potato genotypes, we examined sweet potato regeneration after transformation in five cultivars. An Agrobacterium tumefaciens transformation system was used for the introduction of mammalian cytochrome P450 genes, which are capable of conferring herbicide tolerance. Among the different factors studied, including explant type, plasmid vectors, and auxin type in the initiation media, auxin type had the greatest effect on the regeneration response. Of the auxins tested, only 4-fluorophenoxyacetic acid (4FA) induced regeneration from all cultivars. In terms of the quality of calli, 4FA promoted the induction of type I calli, which were capable of somatic embryo formation, whereas type II calli fail to produce somatic embryos. The frequency of somatic embryo formation was also affected by the composition of the embryo-induction media. Transgenic plants were regenerated from all cultivars. The stable integration and expression of transgenes was confirmed by several approaches. This Agrobacterium-mediated transformation system should be applicable to a wide range of sweet potato cultivars.

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Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis

Cited by 8 publications

References 0 publications

Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Transgenic sweet potato expressing mammalian cytochrome P450

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