Rapid and sensitive anthrone–sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products: Method development and validation

Leyva, Alejandro; Quintana, Anelis; Sánchez, Meily; Rodríguez, E N; Cremata, José A.; Sánchez, Julio César

doi:10.1016/j.biologicals.2007.09.001

Cited by 314 publications

(191 citation statements)

References 23 publications

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“…Total carbohydrates were extracted using hot alkaline solution [18] and estimated by anthrone method [19].…”

Section: Biochemical Characterization Of the Isolatesmentioning

confidence: 99%

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

G¹

2017

International Journal of Research in Environmental Science

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“…Total carbohydrates were extracted using hot alkaline solution [18] and estimated by anthrone method [19].…”

Section: Biochemical Characterization Of the Isolatesmentioning

confidence: 99%

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

G¹

2017

International Journal of Research in Environmental Science

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“…Dry matter content was determined by oven drying the material at 105 °C (Keweiyongxing Instrument Co. Ltd, Beijing, PR China) to constant mass, and subsequently ground in a cutting mill (Kanghe Machinery Manufacturing Co. Ltd, Jiangyin, PR China) for further analysis. Water-soluble carbohydrates were determined by the anthrone-sulfuric acid method (20). The neutral and acid detergent fibre and acid detergent lignin content were determined according to the Van Soest method (21).…”

Section: Chemical Analysismentioning

confidence: 99%

Effects of Formic Acid or Acetic Acid on the Mixed-Storage Quality of Air-Dried Maize Stover and Cabbage Wastes and Microbial Community Analysis

Ren

Wang

Fan

et al. 2018

Food Technol. Biotechnol.

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SUMMARYA mixture of air-dried corn stover and cabbage waste was ensiled to preserve lignocellulosic biomass for use as biofuel. Furthermore, the effects of different fresh mass fractions (0.3 and 0.6 %) of formic or acetic acid on the mixed silage quality were evaluated to guarantee its quality. The application of formic or acetic acid prior to mixing the silage led to higher water-soluble carbohydrate fractions than the negative control, indicating that both acids contributed to preservation of water-soluble carbohydrates during storage for 170 days. The dry matter content was also increased after storage from 90 to 170 days. It was found that the content of neutral and acid detergent fibre, cellulose and holocellulose (the sum of cellulose and hemicellulose) in mixed silage treated with formic or acetic acid was significantly lower than that obtained in the negative control. The pH and the ratio of ammoniacal nitrogen to total nitrogen in mixed silage treated with acetic acid also significantly decreased. Furthermore, the addition of formic or acetic acid significantly weakened the fermentation intensity of lactic acid, depending on the ratio of lactic to acetic acid, as well as the ratio of lactic acid to total organic acids. The number of bacterial species and their relative abundance shifted during silage mixing, wherein microbial communities at phylum level mainly consisted of Proteobacteria and Firmicutes. The dominant bacteria were also observed to shift from Lactobacillus and Enterobacter in presilage biomass to Lactobacillus and Paralactobacillus. Specifically, Enterobacter disappeared after 130 days of storage. In conclusion, the addition of a low dose of acetic acid to fresh mass (0.3 %) could effectively improve the fermentation quality and is conducive to the preservation of the organic components.

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“…Cellular carbohydrates were estimated using the anthrone method [27] after hot alkaline extraction [28]. Briefly, microalgal pellets were resuspended in distilled water and then heated in 40% (w/v) KOH at 90˚C for 1 h. After cooling down, ice cold ethanol was added and stored at −20˚C overnight followed by centrifugation.…”

Section: Carbohydrate Assaymentioning

confidence: 99%

Nutrient Removal Efficiencies of <i>Chlorella vulgaris</i> from Urban Wastewater for Reduced Eutrophication

Singh¹,

Birru²,

Sibi³

2017

JEP

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Urban wastewater contains both organic and inorganic nutrients and discharge of untreated water increases nitrogen and phosphorous content in water bodies leading to eutrophication problem. Physical and chemical treatment of urban waste water produces large quantities of waste sludge associated with secondary pollution. Microalgae can assimilate nutrients especially nitrogen and phosphorous from wastewater for their growth and produce valuable biomass and lipid. This study was performed to determine the growth of Chlorella vulgaris in urban wastewater (UWW) and Bold's basal medium (BBM) thereby identifying cost effective growth medium for microalga cultivation. In addition, nutrient removal abilities of C. vulgaris from various dilutions of urban waste water were explored at 10 days cultivation period. Specific growth rate, biomass and lipid content were higher in microalgae grown in urban waste water than BBM. The highest lipid productivity of 14.31 mg· L −1 ·day −1 was achieved in the culture grown in UWW medium which exceeded the BBM at 1.15 fold. The amount of nutrient removal tended to increase with higher dilutions of UWW. Removal rates of upto 87.9% and 98.4%were recorded for total nitrogen and total phosphorous by C. vulgaris. The results emphasized that urban waste water as a cost effective growth medium for higher biomass and lipid production accompanied with the nutrient removal efficiency of microalgae to reduce eutrophication.

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Rapid and sensitive anthrone–sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products: Method development and validation

Cited by 314 publications

References 23 publications

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

Effects of Formic Acid or Acetic Acid on the Mixed-Storage Quality of Air-Dried Maize Stover and Cabbage Wastes and Microbial Community Analysis

Nutrient Removal Efficiencies of <i>Chlorella vulgaris</i> from Urban Wastewater for Reduced Eutrophication

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Rapid and sensitive anthrone–sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products: Method development and validation

Cited by 314 publications

References 23 publications

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

Fruit Wastes Hydrolysates as Feedstock: Pre-Treatment Strategies for Cost-Saving and Sustainable Microalgae Cultivation

Effects of Formic Acid or Acetic Acid on the Mixed-Storage Quality of Air-Dried Maize Stover and Cabbage Wastes and Microbial Community Analysis

Nutrient Removal Efficiencies of &lt;i&gt;Chlorella vulgaris&lt;/i&gt; from Urban Wastewater for Reduced Eutrophication

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Nutrient Removal Efficiencies of <i>Chlorella vulgaris</i> from Urban Wastewater for Reduced Eutrophication