Rapid and Sensitive Competitive Fluorescence Immunoassay for the Detection of Chlortetracycline Residues in Environment

Abstract

An direct competitive immunofluorescence method was developed in plate to detect chlortetracycline residues in environment using the Lumichrome as fluorescent probe. Different reaction conditions such as pH , the concentration of antigen and antibody and incubation times were assayed. Under optimized conditions, chlortetracycline can be determined in the concentration range of 1×10-8--2×10-4 mol/L, and the linear equation was y=11.363x+93.026, R2=0.99. The detection limit of this method IC90 was 3.74×10-7mol/L. The recoveries of chlortetracycline at various spiking levels in the sludge samples ranged from 95.43% to 104.26%, with relative standard deviations of 3% to 6%. These data demonstrate that the excellent performance of the proposed method in the detection of chlortetracycline in water samples.