Rapid and Sensitive Detection of Respiratory Virus Molecular Signatures Using a Silver Nanorod Array SERS Substrate

Shanmukh, Saratchandra; Jones, Les; Driskell, Jeremy D.; Zhao, Yiping; Dluhy, Richard A.; Tripp, Ralph A.

doi:10.1021/nl061666f

Cited by 593 publications

(453 citation statements)

References 33 publications

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“…is crucial in life sciences and medical diagnostics (1-5, 44, 45). Over the years, SERS has already proven to be a powerful platform to detect and analyze various biological entities such as proteins (46,47), viruses (48), and cells (49) as well as DNA (e.g., discriminate mutations) (50, 51) and RNA (11). As proof-ofconcept examples, we have shown that DNA bases of thymine and adenine as well as proteins such as bovine serum albumin (BSA) can be detected in water at subfemtomolar concentrations without additional labeling processes using SLIPSERS (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Yang

Dai

Stogin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

634

442

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Detecting target analytes with high specificity and sensitivity in any fluid is of fundamental importance to analytical science and technology. Surface-enhanced Raman scattering (SERS) has proven to be capable of detecting single molecules with high specificity, but achieving single-molecule sensitivity in any highly diluted solutions remains a challenge. Here we demonstrate a universal platform that allows for the enrichment and delivery of analytes into the SERSsensitive sites in both aqueous and nonaqueous fluids, and its subsequent quantitative detection of Rhodamine 6G (R6G) down to ∼75 fM level (10 −15 mol·L −1 ). Our platform, termed slippery liquidinfused porous surface-enhanced Raman scattering (SLIPSERS), is based on a slippery, omniphobic substrate that enables the complete concentration of analytes and SERS substrates (e.g., Au nanoparticles) within an evaporating liquid droplet. Combining our SLIPSERS platform with a SERS mapping technique, we have systematically quantified the probability, p(c), of detecting R6G molecules at concentrations c ranging from 750 fM (p > 90%) down to 75 aM (10 −18 mol·L −1 ) levels (p ≤ 1.4%). The ability to detect analytes down to attomolar level is the lowest limit of detection for any SERS-based detection reported thus far. We have shown that analytes present in liquid, solid, or air phases can be extracted using a suitable liquid solvent and subsequently detected through SLIPSERS. Based on this platform, we have further demonstrated ultrasensitive detection of chemical and biological molecules as well as environmental contaminants within a broad range of common fluids for potential applications related to analytical chemistry, molecular diagnostics, environmental monitoring, and national security.spectroscopy | sensing | SERS | slippery surfaces | nanoparticles

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Yang

Dai

Stogin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

634

442

View full text Add to dashboard Cite

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“…Silver nanoparticles are widely used in surface enhanced Raman scattering (SERS) spectroscopy (surface-sensitive technique which is known for its high sensitivity due to its surface plasmon effects lacks behind at uniform substrate formation) because of high local field enhancement factor. For example, it was demonstrated that SERS and silver nanorods quickly revealed the viral structures (Shanmukh et al 2006). It can also differentiate between respiratory viruses, virus strains, and viruses containing gene deletions without manipulating the virus.…”

Section: Applications Of Silver Nanoparticles and Their Incorporationmentioning

confidence: 99%

Silver Nanoparticles

Korbekandi¹,

Iravani²

2012

The Delivery of Nanoparticles

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“…This novel SERS assay can detect spectral differences between viruses, viral strains and viruses with gene deletions in biological media. The method provides rapid detection and characterization of viruses generating reproducible spectra without viral manipulation (Shanmukh et al, 2006). Persistent infection with hepatitis B virus [HBV] is a major health problem worldwide and may lead to chronic hepatitis, cirrhosis and primary liver cancer (Ye et al, 2003).…”

Section: Avian Influenza Virus [Aiv]mentioning

confidence: 99%