Rapid and sensitive detection of white spot syndrome virus by loop-mediated isothermal amplification combined with a lateral flow dipstick

Jaroenram, Wansadaj; Kiatpathomchai, Wansika; Flegel, Timothy W.

doi:10.1016/j.mcp.2008.12.003

Cited by 140 publications

(80 citation statements)

References 25 publications

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“…The maximum fluorescence intensity is 6.5 folds compared with the initial intensity (at 0 minute). Thus, the overall of reaction can be done within 10 minutes, which is relatively fast compared with some other DNA detection methods (Jaroenram et al, 2009).The fluorescence recovery can be attributed to the release of FITC-probe from GO diminishing the fluorescence quenching via energy transfer process. The formation of dsDNA-FITC complex reduces the surface charge of DNA molecules leading to the exposure of DNA bases, which weaken the π-π stacking and hydrogen bonding interactions (Dong et al, 2010).…”

Section: Optimization Of Hybridization Timementioning

confidence: 98%

Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Waiwijit

Phokaratkul

Kampeera

et al. 2015

Journal of Biotechnology

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Section: Optimization Of Hybridization Timementioning

confidence: 98%

Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Waiwijit

Phokaratkul

Kampeera

et al. 2015

Journal of Biotechnology

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“…The entire operation is simple, safe with visible results. LAMP-LFD technology has been applied by many researchers and widely used in the detection of fungi, bacteria and viruses (Jaroenram et al, 2009;Nimitphak et al, 2010;Plaon et al, 2011). This method has great promotion prospect in the rapid biological detection.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick

Li¹,

Xiong²,

Zhu³

et al. 2018

American Journal of Biochemistry and Biotechnology

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Abstract:The existence of Sulfate-Reducing Bacteria (SRB) is one of the significant reasons for the Microbially Influenced Corrosion (MIC) of jet fuel. Especially for the jet fuel stored by military, since jet fuel is stored in the tank for a long time, some oxygen-consuming bacteria such as Amorphotheca resinae and Bacillus Cohn can consume oxygen and generate organic acids at the oilwater interface of the tank bottom. This causes anaerobic SRB flourish in fuel tanks. In this study, a loop-mediated isothermal amplification (LAMP) combined with a chromatographic Lateral Flow Dipstick (LFD) assay was established to detect the SRB. Four groups of LAMP primers were designed and synthesized to target dsrB (dissimilatory sulfite reductase β-subunit) genes in SRB.LAMP-LFD can detect 121 fg/µL of SRB DNA within 35 min. The detection limit of this method is 1000 times more sensitive than the conventional PCR and shortens the detection time greatly. This method is negative for other eight common bacteria species in jet fuel, indicating that the method has high specificity. In summary, this method can be used to detect the presence of SRB in jet fuel.

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“…It has been broadly used for detecting and monitoring infections and aquatic viral load; however, these techniques require centralized laboratory facilities and clinical specimen submissions that might delay disease diagnosis. In recent years, an elegant and innovative technique called the loop-mediated isothermal amplification (LAMP) method has been successfully applied for detecting many kinds of aquatic bacteria and virues, such as Flavobacterium psychrophilum (Fujiwara and Eguchi 2009), Vibrio alginolyticus (Cai et al 2010), haematopoietic necrosis virus (IHNV) (Gunimaladevi et al 2005), haemorrhagic septicaemia virus (VHS) (Soliman and El-Matbouli 2006), yellow head virus (YHV) (Mekata et al 2006), spring viraemia of carp virus (SVCV) (Shivappa et al 2008), and white spot syndrome virus (Jaroenram et al 2009). The RT-LAMP, utilizing two sets of primers including two inner primers and two outer primers, are specific for six independent regions of the target sequence.…”

mentioning

confidence: 99%

Comparison of reverse-transcription loop-mediated isothermal amplification and reverse-transcription polymerase chain reaction for grass carp reovirus

2015

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Grass carp reovirus (GCRV) has been assigned to a newly established Aquareovirus genus in the family of Reoviridae which leads to haemorrhagic disease and extremely high mortality rate in grass carp. In this study, comparison was made between the novel one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the reverse transcription polymerase chain reaction (RT-PCR) for detection of grass carp reovirus. The result indicated that RT-LAMP had × 10 higher sensitivity comparable to RT-PCR. The specificity of the two methods for GCRV detection were both developed successfully by other three aquatic viruses. In the field trial, both RT-PCR and RT-LAMP methods were applied to detect the samples from different infected organs and tissues. The result demonstrated that RT-LAMP had a high accuracy to confirm the diagnosis as well as the RT-PCR. This study showed that the RT-LAMP, compared to the RT-PCR, was simple, time-saving, convenient, but required specificity primers and possibly generated false positive product. Its products, unlike RT-PCR, could not be direcly used in further molecular research after purification. Thus RT-LAMP might be an optimal diagnostic method for rapid and preliminary diagnosis of GCRV infection in resource-limited setting situation.

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Rapid and sensitive detection of white spot syndrome virus by loop-mediated isothermal amplification combined with a lateral flow dipstick

Cited by 140 publications

References 25 publications

Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Sensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick

Comparison of reverse-transcription loop-mediated isothermal amplification and reverse-transcription polymerase chain reaction for grass carp reovirus

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