Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification

Wang, Xuan; FengGui, Yin; Bi, Yang; Cheng, Gong; Li, Jing; Hou, Lihua; Li, Yunlong; Yang, Baozhi; Liu, Wenjun; Yang, Limin

doi:10.1016/j.jviromet.2016.10.010

Cited by 68 publications

(68 citation statements)

References 33 publications

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“…Importantly, the simple, single-temperature incubation allows LAMP reactions to be performed without expensive equipment, directly in the field [36]. Since the 2015 emergence of the ZIKV in Brazil, many LAMP assays have been developed for diagnosis by research groups across the world [27,[37][38][39][40][41][42][43][44][45]. These diagnostic platforms based on LAMP have proven to be specific, sensitive and inexpensive POC tools that can be applied even in resource-limited regions of the world.…”

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Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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“…[4] While reverse transcription PCR (RT-PCR) is often used as aN AT,R T-LAMP has also been extensively used for virus detection owing to its robustness,h igh sensitivity and specificity,s hort incubation time,a nd simplified thermal management. [7][8][9][10][11][12][13][14] Although RT-LAMP allows virus samples to be used for amplification without sample preparation, the small sample volume and dilution steps involved decrease detection sensitivity. [7][8][9][10][11][12][13][14] Although RT-LAMP allows virus samples to be used for amplification without sample preparation, the small sample volume and dilution steps involved decrease detection sensitivity.…”

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“…[5,6] Several RT-LAMP tests have been developed to specifically detect ZIKV RNA, especially for point-of-care (POC) diagnosis. [10,15] On the other hand, traditional RNApreparation methods such as solid-phase extraction (SPE) are timeconsuming and labor-intensive.T he requirement for centrifugation equipment, various types of reagents,a nd ac ontrolled environment makes it unrealistic for the operation outside alab. [10,15] On the other hand, traditional RNApreparation methods such as solid-phase extraction (SPE) are timeconsuming and labor-intensive.T he requirement for centrifugation equipment, various types of reagents,a nd ac ontrolled environment makes it unrealistic for the operation outside alab.…”

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Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

et al. 2018

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The recent outbreaks of Zika virus (ZIKV) infection represent ap ublic health challenge.R apid, cost-effective,a nd reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable,e specially for resource-limited nations.T oaddress the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and apaper-based unit for RNAenrichmentand purification. The paper unit is placed in ac ommercially available coffee mug that provides ac onstant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or ac ellphone camera. Using the device,w ed emonstrated the reproducible detection of ZIKV in human urine and saliva samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…RT-LAMP was established for Dengue virus (Hu et al 2015;Lau et al 2015), influenza viruses (Nakauchi et al 2014;Bao et al 2015;Kim et al 2016a), Hepatitis C (Nyan and Swinson 2016;Zhao et al 2017), Ebola virus (Oloniniyi et al 2017), respiratory syncytial virus (Hoos et al 2017), zika virus (Tian et al 2016;Wang et al 2016;Chotiwan et al 2017) and Middle East Respiratory Syndrome coronavirus (Mers-CoV) Lee et al 2016). Apart from helping in diagnosis, the RT-LAMP can also be used as an epidemiologic surveillance system for human virus infections.…”

Section: Reverse Transcription Lampmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

et al. 2018

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Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification

Cited by 68 publications

References 33 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

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