Rapid and Sensitive Detection of Rutin in Food Based on Nitrogen-Doped Carbon Quantum Dots as Fluorescent Probe

Abstract

The aim of this study was to establish a rapid detection method of rutin in food based on nitrogen-doped carbon quantum dots (N-CDs) as the fluorescent probe. N-CDs were prepared via a single-step hydrothermal process using citric acid as the carbon source and thiourea as the nitrogen source. The optical properties of N-CDs were characterized using an electron transmission microscope, X-ray diffractometer, Fourier-transform infrared spectrometer, and nanoparticle size potential analyzer. The UV/Vis absorption property and fluorescence intensity of N-CDs were also characterized using the respective spectroscopy techniques. On this basis, the optimal conditions for the detection of rutin by N-CDs fluorescent probes were also explored. The synthesized N-CDs were amorphous carbon structures with good water solubility and optical properties, and the quantum yield was 24.1%. In phosphate-buffered solution at pH = 7.0, Rutin had a strong fluorescence-quenching effect on N-CDs, and the method showed good linearity (R2 = 0.9996) when the concentration of Rutin was in the range of 0.1–400 μg/mL, with a detection limit of 0.033 μg/mL. The spiked recoveries in black buckwheat tea and wolfberry were in the range of 93.98–104.92%, the relative standard deviations (RSD) were in the range of 0.35–4.11%. The proposed method is simple, rapid, and sensitive, and it can be used for the rapid determination of rutin in food.

Preparation of carbon quantum dot fluorescent probe from waste fruit peel and its use for the detection of dopamine

“…Additionally, a small cluster of absorption peaks appeared at approximately 360 nm, possibly indicating that the electronic structure in the CQDs had been altered by N doping through C N bonding, resulting in the generation of n–π* transitions and the observed absorption peaks. 30 One hundred microliters of the prepared N-CQDs were taken and diluted for the measurements. A PL was used to measure the maximum excitation and emission wavelengths of the samples.…”

Recent Advances in Functional Nanomaterials for Electrochemical Sensors and Biosensors

“…Huang et al established a rapid detection method for rutin in food using nitrogendoped carbon quantum dots as the fluorescent probe [10]. In their work, nitrogen-doped carbon quantum dots were prepared via a single-step hydrothermal process, using citric acid as the carbon source and thiourea as the nitrogen source.…”

Portable ratiometric fluorescence detection of Cu2+ and thiram

“…The broad absorption band observed near 3185 cm −1 can be attributed to the N-H/O-H group stretching vibration [32]. The absorption band around 1573 cm −1 and 1401 cm −1 are contributed from N-H vibrations and C-O stretching, respectively [34,35]. In addition, C-N vibrations at 1259 cm −1 , -CH-OH vibrations at 1078 cm −1 , and C-O stretching at 1049 cm −1 were observed [36][37][38].…”