Rapid and sensitive LC‐MS/MS method for quantification of lamotrigine in human plasma: application to a human pharmacokinetic study

Hotha, Kishore Kumar; Kumar, S. Sirish; Bharathi, D. Vijaya; Venkateswarulu, V.

doi:10.1002/bmc.1692

Cited by 25 publications

(12 citation statements)

References 15 publications

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“…The calibration curve of PU‐48 showed good linearity at concentrations ranging from 0.1 to 1000 ng/mL in rat plasma with correlation coefficient ( r 2 ) exceeding 0.99. The present result supported the observations that linear responses could be achieved using API 4000 LC‐ESI‐MS/MS in the range of 0.1–1500 or 0.3–3000 ng/mL (Feng, Chen, Lin, & Liu, ; Hotha, Kumar, Bharathi, & Venkateswarulu, ). The average regression equation of the calibration curves was y = 0.00688 x + 0.00104 ( r 2 = 0.9942, n = 3), where y represents the peak area ratio of PU‐48 to that of IS and x represents the concentration of PU‐48.…”

Section: Resultssupporting

confidence: 89%

Development and validation of an LC‐MS/MS method for the determination of a novel thienoquinolin urea transporter inhibitor PU‐48 in rat plasma and its application to a pharmacokinetic study

Zhang

Wang

Liu

et al. 2017

Biomedical Chromatography

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A specific, sensitive and stable high-performance liquid chromatographic-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantitative determination of methyl 3-amino-6-methoxythieno [2,3-b]quinoline-2-carboxylate (PU-48), a novel diuretic thienoquinolin urea transporter inhibitor in rat plasma. In this method, the chromatographic separation of PU-48 was achieved with a reversed-phase C column (100 × 2.1 mm, 3 μm) at 35°C. The mobile phase consisted of acetonitrile and water with 0.05% formic acid added with a gradient elution at flow rate of 0.3 mL/min. Samples were detected with the triple-quadrupole tandem mass spectrometer with multiple reaction monitoring mode via electrospray ionization source in positive mode. The retention time were 6.2 min for PU-48 and 7.2 min for megestrol acetate (internal standard, IS). The monitored ion transitions were mass-to-charge ratio (m/z) 289.1 → 229.2 for PU-48 and m/z 385.3 → 267.1 for the internal standard. The calibration curve for PU-48 was linear over the concentration range of 0.1-1000 ng/mL (r > 0.99), and the lower limit of quantitation was 0.1 ng/mL. The precision, accuracy and stability of the method were validated adequately. The developed and validated method was successfully applied to the pharmacokinetic study of PU-48 in rats.

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Section: Resultssupporting

confidence: 89%

Development and validation of an LC‐MS/MS method for the determination of a novel thienoquinolin urea transporter inhibitor PU‐48 in rat plasma and its application to a pharmacokinetic study

Zhang

Wang

Liu

et al. 2017

Biomedical Chromatography

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“…Thus, we considered reverse‐phase extraction with a hydrophobic solid phase, normal‐phase extraction with a hydrophilic solid phase, and ionic and polar separation. Several previous studies have achieved LC separation with C8, C18, or cyanosolid phases. Moreover, in experiments using a normal‐phase column with a silica column and aqueous‐acetonitrile mobile phases, researchers established systems for determining basic compounds that contained guanidine groups and are structurally similar to LTG .…”

Section: Discussionmentioning

confidence: 99%

Determination of lamotrigine in human plasma using liquid chromatography‐tandem mass spectrometry

Shogo

Rina

Nishina

et al. 2019

Neuropsychopharm Rep

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Aim Lamotrigine (LTG) is a widely used anti‐epileptic drug that is administered to avoid seizures and to maintain seizure‐free status. However, several factors reportedly cause individual differences of plasma LTG levels, and the therapeutic target range of LTG varies between individuals. Thus, to optimize effective doses of LTG, we developed a rapid and simple method for determining plasma LTG concentrations. Methods Lamotrigine and the internal standard papaverine were extracted from human plasma using solid‐phase extraction. After filtration, 5‐μL aliquots of final samples were injected into the liquid chromatography‐tandem mass spectrometry instrument and LTG and internal standard were separated using a Cadenza CD‐C18 column (100 × 2 mm, 3 μm) with 0.1% formic acid in water/acetonitrile (2/1, v/v). Results The calibration curve was linear from 0.2 to 5.0 μg/mL, and assessments of recovery, intra‐ and inter‐day precision and accuracy, matrix effects, freeze and thaw stability, and long‐term stability demonstrated good reproducibility. Retention times of LTG and internal standard were 1.6 and 2.0 minutes, respectively, and the total run time was 3.5 minutes for each sample. Conclusion We developed a rapid and simple method for determining plasma LTG concentrations. The present novel system could be used to inform LTG dose adjustments for individual patients.

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“…Very few methods employing LC-MS/MS have been published for the determination of lamotrigine in human plasma. [26][27][28] www.wjpps.com Different individual lots of K 3 EDTA (ethylene diamine tetra acetic acid tri potassium salt)…”

Section: Introduction Fig 1: Chemical Structure Of Lamotriginementioning

confidence: 99%

Development and Validation of Analytical Method for the Estimation of Lamotrigine in Human Plasma

Kumar¹

2017

WJPPS

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A Simple, rapid, selective and sensitive liquid chromatographic mass spectrometry method was developed and validated for the determination of Lamotrigine from human plasma. The drug was extracted by ethyl acetate. Lamotrigine was measured in plasma using a validated method with mass spectrometer detector. Chromatographic peaks were separated on 4μm Chromolith, RP C-18 (504.6mm, 4µ) using 80:20 v/v Phosphate buffer pH 2.5, Acetonitrile asmobile phase at a flow rate of 0.500 ml/min. The chromatograms showed good resolution and no interference from plasma. The mean recovery from human plasma was found to be above 85%. The method was linear over the concentration range of 5 μg/ml to 1200 μg/ml with coefficient of correlation (r2) 0.9987. Both intraday and interday accuracy and precision data showed good reproducibility. This method was successfully applied to pharmacokinetics studies.

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Rapid and sensitive LC‐MS/MS method for quantification of lamotrigine in human plasma: application to a human pharmacokinetic study

Cited by 25 publications

References 15 publications

Development and validation of an LC‐MS/MS method for the determination of a novel thienoquinolin urea transporter inhibitor PU‐48 in rat plasma and its application to a pharmacokinetic study

Development and validation of an LC‐MS/MS method for the determination of a novel thienoquinolin urea transporter inhibitor PU‐48 in rat plasma and its application to a pharmacokinetic study

Determination of lamotrigine in human plasma using liquid chromatography‐tandem mass spectrometry

Development and Validation of Analytical Method for the Estimation of Lamotrigine in Human Plasma

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