Rapid and specific detection of Fusarium acuminatum and Fusarium solani associated with root rot on Astragalus membranaceus using loop-mediated isothermal amplification (LAMP)

Wang, Yan; Wang, Chunwei; Ma, Yurong; Zhang, Xiqian; Yang, Hong-Chang; Li, Guangxin; Li, Xinfeng; Wang, Meiqin; Zhao, Xiaojun; Wang, Jianming; Zhang, Xianhong

doi:10.1007/s10658-022-02478-x

Cited by 8 publications

(2 citation statements)

References 54 publications

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“…Fusarium solani causes soybean root rot and blocks the absorption of nutrients and water transport in the root, resulting in stunting and wilting of soybean plants, even death, and substantial economic losses to farmers . Under the same conditions, the antifungal activity of PTC@FL-MSNs against soybean root rot fungus was obviously better than that of the technical material of PTC (Figure A,B).…”

Section: Resultsmentioning

confidence: 99%

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Wang

Zhao

et al. 2023

J. Agric. Food Chem.

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Prothioconazole (PTC) has been widely utilized for plant fungal disease control, but its metabolite prothioconazoledesthio (PTC-d) exhibits reproductive toxicity. In the present study, carbon quantum dot (CQD)-modified fluorescent doublehollow shelled mesoporous silica nanoparticles (FL-MSNs) loaded with PTC, referred to as PTC@FL-MSNs, were constructed with an average size of 369 nm and a loading capacity of 28.1 wt %, which could increase the antifungal efficiency of PTC. In addition, upright fluorescence microscope and UPLC-MS/MS studies showed that PTC@FL-MSNs could be effectively transported via root uptake and foliar spray in soybean plants. Compared to a 30% PTC dispersible oil suspension agent, the PTC@FL-MSN treatment group showed higher concentrations (leaves: 0.50 > 0.48 mg/kg), longer half-lives for degradation (leaves: 3.62 > 3.21 d; roots: 3.39 > 2.82 d), and fewer metabolites. These findings suggest that sustained pesticide release and toxicity reduction are potential applications for PTC nanofungicide delivery technology.

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Section: Resultsmentioning

confidence: 99%

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Wang

Zhao

et al. 2023

J. Agric. Food Chem.

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“…Therefore, it is necessary to develop a LAMP assay with high specificity. In the research, two new LAMP assays were developed to detect Fusarium acuminatum and F. solani based on the partial translation elongation factor-1 alpha (TEF-1 alpha) gene region [36]. These two rapid and specific LAMP assays could be applied for direct detection of F. acuminatum and F. solani on Astragalus membranaceus [36].…”

Section: Discussionmentioning

confidence: 99%

Development of Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Pyrenophora graminea in Barley Seeds

Chen

et al. 2022

Agronomy

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Barley leaf stripe, caused by Pyrenophora graminea, is an essential systemic seed-borne disease in barley worldwide. Barley is a major cereal crop in the Qinghai–Tibet Plateau, and barley production has been threatened by leaf stripe in this region, particularly in organic farming regions. Detecting the pathogen in infected barley seeds is crucial for managing barley leaf stripe. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect the pathogen based on primers designed based on the sequence of the pig 14 gene (GenBank: AJ277800) of P. graminea. The optimal concentrations of MgSO4, dNTPs, and enzymes in the LAMP reaction system were established as 10.0 mM, 1.0 mM, and 8 U in a 25 μL reaction volume, respectively. The established LAMP methods for detecting P. graminea were optimally performed at 63 °C for 70 min with high reliability. The minimum detection limit was 1 × 10−2 ng·μL−1 in the 25 uL reaction system. The specificity of LAMP for P. graminea was validated with eight fungal species. All DNA extracts from P. graminea-infected barley seeds with incubation, intact, and smashed treatments were applied in LAMP and confirmed to enable the detection of the pathogen. The LAMP assay in this study could facilitate the detection of P. graminea in barley seeds onsite, provide information for seed health certificates, and help decide on seed treatment in leaf stripe management.

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Loop-mediated isothermal amplification (LAMP) and suction spore trap-based assay for detection of mildew pathogens Podosphaera xanthii and Pseudoperonospora cubensis in cucurbits

Palanisamy,

Karthikeyan,

Jaffer

et al. 2025

Crop Protection

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Rapid and specific detection of Fusarium acuminatum and Fusarium solani associated with root rot on Astragalus membranaceus using loop-mediated isothermal amplification (LAMP)

Cited by 8 publications

References 54 publications

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Development of Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Pyrenophora graminea in Barley Seeds

Loop-mediated isothermal amplification (LAMP) and suction spore trap-based assay for detection of mildew pathogens Podosphaera xanthii and Pseudoperonospora cubensis in cucurbits

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