Rapid and specific electrochemical detection of prostate cancer cells using an aperture sensor array

Abstract

A rapid, simple and specific cancer cell counting sensor would allow for early detection and better disease management. We have developed a novel cell counting device that can specifically count 125 prostate cancer cells in both complex media with serum and a mixed cell population containing non-target cells within 15 min. The microfabricated glass chip with exposed gold apertures utilizes the anti-EpCAM antibody to selectively count prostate cancer cells via differential pulse voltammetry. The newly developed sensor exhibits excellent sensitivity and selectivity. The cells remain viable throughout the counting process and can be used for further analysis. This device could have utility for future applications in early stage cancer diagnosis.

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

“…Impedanzsensoren wurden für die CTC-Identifizierung benutzt, [31,93] und Voltammetriesensoren sind spezifisch zur Identifizierung von Krebszellen geeignet. [94][95][96][97] Jüngst wurde gezeigt, wie die Aufarbeitung von Vollblut, die Gewinnung von CTCs,d ie Zelllyse und die Analyse der mRNA-Expression mit einem Chip-basierten elektrochemischen Detektor ablaufen kann (Abbildung 6A). [98] Der gesamte Prozess wurde auf einem einzigen integrierten Chip durchgeführt und war innerhalb von 30 Minuten abgeschlossen.…”

Detection of 17 β-estradiol in Environmental Estrogen Pollution and in Women’s Health Care Using a Single-Use, Cost Effective Biosensor Based on Differential Pulse Voltammetry (DPV)

“…MPA molecule consisted of a thiol functional group at one end, which provided an excellent affinity to gold, and a carboxylic group at another end, which was suitable for bonding covalently to proteins through peptide bond after an activation procedure. Thiol modification of gold electrode surface for protein immobilization was a well-acknowledged technique (32,33,(36)(37)(38). Typically, 4-8 biosensor were prepared in this immobilization step as a batch for this study.…”

Folic acid modified clay/polymer nanocomposites for selective cell adhesion

“…41,42 These surfaces could be adapted properly to 'Lab-ona-Chip' or microuidic based systems. 43 On the other hand, decision on the immobilization matrix affects the selectivity of the biosensor for the analysis, signicantly. Herein, aer the successful cell adhesion experiments, NC-8 was applied to create a novel selective platform for the cultivation of folate positive HeLa cells.…”