Rapid and visual detection of berberine hydrochloride based on a water‐soluble pyrene derivative

Abstract

In this study, a rapid method for the detection of berberine hydrochloride (BRH) was developed based on a water-soluble pyrenyl probe, 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS). This method features low cost, good selectivity, high sensitivity and a fast response. The sensing mechanism of this probe is attributed to the formation of a complex between HPTS and BRH induced by electrostatic interaction and π-π stacking. To the best of our knowledge, this is the first fluorescent sensor for BRH based on organic materials that has low cost and a visual response. The detection limit of this method was as low as 1.24 μM and the linear response range is 2-50 μM. This method also allowed rapid detection of BRH real samples.

Cavity hairpin ThT-light nucleic acid switches: the construction of label- and enzyme-free sensing and imaging platforms

“…Based on its own characteristics, berberine mostly acts as a fluorescent molecule in a label-free sensor to report the detection signal, while for its own detection, it still stays in the stage of relying on large-scale instruments, such as high-performance liquid chromatography ( 52 ), mass spectrometry ( 53 ) and capillary electrophoresis ( 54 ), although the accuracy is high, the analysis cost is expensive and cannot meet the needs of large-scale on-site detection. Even if some receptors of macrocyclic aromatic hydrocarbons, such as cucurbituril ( 55 , 56 ) and pyrene derivative ( 57 ), have been found enable to excite BB fluorescence and achieving sensing, their specificity and sensing ability cannot be directly proportional to its complex synthesis process ( 58 ). At present, a simpler and more portable DNA-mediated label-free BB sensor needs to be developed.…”

New pyrrolo[3,2‐b]pyrroles with AIE characteristics for detection of dichloromethane and chloroform

“…Molecules exhibiting AIE character have been used as components in organic light-emitting diodes (OLEDs) and field effect transistors and as fluorescent probes [23][24][25].…”

“…Over the years, such skeletons, that is, hexaphenylsiloles, TPE derivatives, polyphenylenes, tetraphenylthieno[3,2‐b]thiophene S , S ‐dioxide, and diphenylbenzo[b]thiophene S , S ‐dioxide have been widely used in the design of new AIEgens [19–22]. Molecules exhibiting AIE character have been used as components in organic light‐emitting diodes (OLEDs) and field effect transistors and as fluorescent probes [23–25].…”

A fluorometric and colorimetric approach for the rapid detection of berberine hydrochloride based on an anionic polythiophene derivative

“…To date, several methods have been explored for quantification of BRH, including resonance light scattering spectrometry (RLS), [ 14 ] chemiluminescence, [ 15,16 ] high performance liquid chromatography (HPLC), [ 17,18 ] capillary electrophoresis, [ 19,20 ] electrochemical analysis, [ 21 ] and optical detection based on nanoparticles or conjugated materials. [ 22–25 ] Among the various strategies mentioned above, optical assays based on synthetic colorimetric and fluorescent probes have received considerable attention due to their high sensitivity, selectivity, and simplicity. [ 26–31 ] Furthermore, optical measurement methods avoid some of the drawbacks of other detection methods including expensive equipment and complicated operations.…”