Rapid antimicrobial susceptibility testing by matrix-assisted laser desorption ionization–time of flight mass spectrometry using a qualitative method in Acinetobacter baumannii complex

Li, Mengjiao; Liu, Minxue; Song, Qifei; Xiong, Li; Chen, Zhuo; Kang, Mei; Xie, Yi

doi:10.1016/j.mimet.2018.09.002

Cited by 11 publications

(10 citation statements)

References 15 publications

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“…Previous studies have demonstrated that a minimum incubation time of 4 h is optimum for the reliable detection of bacterial growth. 18 , 23 To simplify the method, we directly centrifuged 96-well plates at 4000 rpm for 10 minutes to subside the bacterial cells rather than remove the suspension into the new 1.5 mL tube following centrifugation for 3 minutes at 12,000 rpm. The same parallel test was performed in which the suspension was pipetted into a tube and centrifuged at 12,000rpm for 3 minutes.…”

Section: Discussionmentioning

confidence: 99%

“…Also, a new mass spectrometry-based method has been proposed by combining MBT-ASTRA and DOT-MGA for the rapid determination of MIC values. 23 Klebsiella pneumoniae is one of the most common opportunistic pathogens found in the clinic. As a consequence of the over-use of cephalosporins, carbapenems-resistant K. pneumoniae (CRKP) has emerged making the treatment of multidrug resistance very challenging.…”

Section: Introductionmentioning

confidence: 99%

“…Also, a new mass spectrometry-based method has been proposed by combining MBT-ASTRA and DOT-MGA for the rapid determination of MIC values. 23 …”

Section: Introductionmentioning

confidence: 99%

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A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a novel method that can be used to identify pathogens and has potential applications in the detection of drug-resistant bacteria. Purpose: To evaluate the ability of a MALDI-TOF MS-based broth micro-dilution method in detecting the minimum inhibitory concentration (MIC) values of Klebsiella pneumoniae to ceftriaxone and imipenem. Materials and Methods: Sixty strains of K. pneumoniae with different levels of resistance to carbapenems and cephalosporins were randomly collected. The 0.5 McFarland (Mc) concentration of the bacterial suspension was inoculated in cation-adjusted Mueller-Hinton broth (CAMHB) with a final cell turbidity of 5×10 5 CFU/mL. The broth was incubated with serial concentrations of antibiotics. After centrifuging the bacterial suspensions, the lysed cells were analyzed by MALDI-TOF MS to identify the growth-promoting or inhibitory effects on K. pneumoniae. The molecular mechanisms of resistance were investigated by PCR and DNA sequencing analysis. Results: The expression of known resistance genes (blaKPC, blaFOX, blaDHA, blaCTX-M and blaTEM) was detected in the 30 carbapenems-resistant strains. The agreement between the MIC values derived from the MALDI-TOF MS analysis and from the broth microdilution method was 61.7% for ceftriaxone and 71.7% for imipenem. According to the Clinical and Laboratory Standards Institute (CLSI) breakpoint of resistance to ceftriaxone and imipenem, the 60 isolates were accurately classified as resistant or susceptible isolates with 100% sensitivity and 100% specificity. Conclusion:The transmission and infection of multidrug-resistant bacteria could be better managed and treated with the rapid identification of strains and antimicrobial susceptibility. A MALDI-TOF MS-based susceptibility test could be used to identify resistance of K. pneumoniae within a short time-frame. This approach could potentially be used as a supplementary antimicrobial susceptibility test that could be investigated on more bacterial species combined with different antibiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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“…These are time-consuming processes where time can be the difference between lives saved or lives lost. Newer methods to accelerate this process require costly equipment and special expertise [29], [30]. Similarly, there is need to differentiate between dead and live bacteria after processes intended to sterilize lifesaving fluids or surgical equipment [31] including products intended for ingestion [32], [33].…”

Section: Introductionmentioning

confidence: 99%

Identification of Live and Dead Bacteria: A Raman Spectroscopic Study

Dhankhar

Chen

et al. 2019

IEEE Access

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Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules. INDEX TERMS Bacteria inactivation, optical spectroscopy, principal component analysis, Raman scattering.

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“…Another MALDI-TOF MS-based method requires incubation of bacteria with different concentrations of antibiotic, recovery of microorganisms before MS analysis (Li et al, 2018) using the MALDI Biotyper 3.1 software. To evaluate the applicability of the method, meropenem-susceptible and meropenem-resistant A. baumannii clinical isolates were analyzed after a 4 h-incubation with different concentrations of antibiotic.…”

Section: Antibiotic-resistance By Direct-on-target Microdroplet Growtmentioning

confidence: 99%

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Florio

Baldeschi

Rizzato

et al. 2020

Front. Cell. Infect. Microbiol.

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Several MALDI-TOF MS-based methods have been proposed for rapid detection of antimicrobial resistance. The most widely studied methods include assessment of β-lactamase activity by visualizing the hydrolysis of the β-lactam ring, detection of biomarkers responsible for or correlated with drug-resistance/non-susceptibility, and the comparison of proteomic profiles of bacteria incubated with or without antimicrobial drugs. Antimicrobial-resistance to a number of antibiotics belonging to different classes has been successfully tested by MALDI-TOF MS in a variety of clinically relevant bacterial species including members of Enterobacteriaceae family, non-fermenting Gram-negative bacteria, Gram-positive cocci, anaerobic bacteria and mycobacteria, opening this field to further clinically important developments. Early detection of drug-resistance by MALDI-TOF MS can be particularly helpful for clinicians to streamline the antibiotic therapy for a better outcome of patients with systemic infection, in all cases where a prompt and effective antibiotic treatment is essential to preserve organ function and/or patient survival.

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Rapid antimicrobial susceptibility testing by matrix-assisted laser desorption ionization–time of flight mass spectrometry using a qualitative method in Acinetobacter baumannii complex

Cited by 11 publications

References 15 publications

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Identification of Live and Dead Bacteria: A Raman Spectroscopic Study

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

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