Rapid calculation of diffuse reflectance from a multilayered model by combination of the white Monte Carlo and adding-doubling methods

Yoshida, Kenichiro; Nishidate, Izumi

doi:10.1364/boe.5.003901

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Diffuse reflectance spectroscopy has been widely used for the evaluation of human skin chromophores at a single region. [11][12][13][14][15][16][17][18][19] Palmer and Ramanujam 20 have developed a method for the extraction of absorption and scattering coefficients from the spectral diffuse reflectance measured by any arbitrary probe geometry, which is based on an inverse Monte Carlo modeling of the light transport. They also applied the method to a set of diffuse reflectance spectra of breast tissue and demonstrated the ability to classify a sample as malignant or non-malignant with a cross-validated sensitivity and specificity of 82% and 92%, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Measuring and imaging of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

Minakawa,

Wares,

Nakano

et al. 2023

J. Biomed. Opt.

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.SignificanceEvaluation of biological chromophore levels is useful for detection of various skin diseases, including cancer, monitoring of health status and tissue metabolism, and assessment of clinical and physiological vascular functions. Clinically, it is useful to assess multiple different chromophores in vivo with a single technique or instrument.AimTo investigate the possibility of estimating the concentration of four chromophores, bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin from diffuse reflectance spectra in the visible region.ApproachA new diffuse reflectance spectroscopic method based on the multiple regression analysis aided by Monte Carlo simulations for light transport was developed to quantify bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin. Three different experimental animal models were used to induce hyperbilirubinemia, hypoxemia, and melanogenesis in rats.ResultsThe estimated bilirubin concentration increased after ligation of the bile duct and reached around 18 mg / dl at 50 h after the onset of ligation, which corresponds to the reference value of bilirubin measured by a commercially available transcutaneous bilirubin meter. The concentration of oxygenated hemoglobin and that of deoxygenated hemoglobin decreased and increased, respectively, as the fraction of inspired oxygen decreased. Consequently, the tissue oxygen saturation dramatically decreased. The time course of melanin concentration after depilation of skin on the back of rats was indicative of the supply of melanosomes produced by melanocytes of hair follicles to the growing hair shaft.ConclusionsThe results of our study showed that the proposed method is capable of the in vivo evaluation of percutaneous bilirubin level, skin hemodynamics, and melanogenesis in rats, and that it has potential as a tool for the diagnosis and management of hyperbilirubinemia, hypoxemia, and pigmented skin lesions.

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Section: Introductionmentioning

confidence: 99%

“…Diffuse reflectance spectroscopy has been widely used for the evaluation of human skin chromophores at a single region. 11 – 19 …”

Section: Introductionmentioning

confidence: 99%

Measuring and imaging of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

Minakawa,

Wares,

Nakano

et al. 2023

J. Biomed. Opt.

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“…Analysis of diffuse reflectance spectra provide useful information on tissue activities and functions that are related to biological chromophores. Thus, diffuse reflectance spectroscopy (DRS) has been widely used for the evaluating skin chromophores [10][11][12][13][14][15][16][17][18][19][20]. Multi-spectral imaging based on DRS has been employed for visualizing the spatial distribution of chromophore contents in living tissue using a series of discrete optical interference filters, a liquid crystal wavelength tunable filter [21][22][23], and a hyperspectral imaging system [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Simple and affordable imaging of multiple physiological parameters with RGB camera-based diffuse reflectance spectroscopy

Nishidate¹,

Minakawa²,

McDuff³

et al. 2020

Biomed. Opt. Express

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We propose a simple and affordable imaging technique to evaluate transcutaneously multiple physiological parameters by using a digital red-green-blue camera. In this method, the RGB-values were converted into tristimulus values in the CIE (Commission Internationale de l'Eclairage) XYZ color space, which is compatible with the common color spaces. Monte Carlo simulation for light transport in biological tissue was then performed to specify the relationship among the XYZ-values and the concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, bilirubin, and melanin. The concentration of total hemoglobin and tissue oxygen saturation were also calculated from the estimated concentrations of oxygenated and deoxygenated hemoglobin. In vivo experiments with bile duct ligation in rats demonstrated that the estimated bilirubin concentration increased after ligation of the bile duct and reached around 22 mg/dl at 116 h after the onset of ligation, which corresponds to the ground truth value of bilirubin measured by a commercially available transcutaneous bilirubinometer. Experiments with rats while varying the fraction of inspired oxygen demonstrated that oxygenated hemoglobin and deoxygenated hemoglobin decreased and increased, respectively, as the fraction of inspired oxygen decreased. Consequently, tissue oxygen saturation dramatically decreased. We further extended the method to a non-contact imaging photo-plethysmograph and estimation of the percutaneous oxygen saturation. An empirical formula to estimate percutaneous oxygen saturation was derived from the pulse wave amplitudes of oxygenated and deoxygenated hemoglobin. The estimated percutaneous oxygen saturation dropped remarkably when a faction of inspired oxygen was below 19%, indicating the onset of hypoxemia due to hypoxia, whereas the tissue oxygen saturation decreased gradually according to the reduction of the faction of inspired oxygen. The results in this study indicate the potential of this method for imaging of multiple physiological parameters in skin tissue and evaluating an optical biomedical imaging technique that enables cost-effective, easy-to-use, portable, remotely administered, and/or point-of-care solutions.

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The past, present, and prospective on UV–VIS–NIR skin photonics and spectroscopy—a wavelength guide

Poh

Adikan

Moghavvemi

2020

Med Biol Eng Comput

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Rapid calculation of diffuse reflectance from a multilayered model by combination of the white Monte Carlo and adding-doubling methods

Cited by 5 publications

References 19 publications

Measuring and imaging of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

Measuring and imaging of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

Simple and affordable imaging of multiple physiological parameters with RGB camera-based diffuse reflectance spectroscopy

The past, present, and prospective on UV–VIS–NIR skin photonics and spectroscopy—a wavelength guide

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