Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree

Ajithkumar, D.; Seeni, S.

doi:10.1007/s002990050418

Cited by 105 publications

(57 citation statements)

References 11 publications

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“…In recent years, there has been an increased interest in in vitro culture techniques which offer a viable tool for mass multiplication and germplasm conservation of rare, endangered, and threatened medicinal plants (Ajithkumar and Seeni, 1998;Sahoo and Chand, 1998;Prakash et al, 1999). Further, genetic improvement is another approach to augment the drug-yielding capacity of the plant (Tejavathi and Shailaja, 1999).…”

Section: Introductionmentioning

confidence: 99%

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

100

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SummaryAn efficient rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae) by enhanced axillary shoot proliferation in cotyledonary node segments was designed. The medium type, various carbon sources, plant growth regulators, and coconut water markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of benzyladenine (4.4 mM), kinetin (4.6 mM), 2-isopentenyladenine (4.9 mM), gibberellic acid (1.4 mM), 5% coconut water, and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of cotyledonary node segments on a similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (94.3%) on full strength MS medium containing 9.8 mM indolebutyric acid. Micropropagated plants established in garden soil, farmyard soil, and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variation.

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Section: Introductionmentioning

confidence: 99%

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

100

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“…Thus, identification and taxonomic separation of the members of this genus will be difficult (Kaloo et al 2012). Recently, in plant biotechnology, in vitro culture techniques have become more frequently used offering a viable tool for mass multiplication and germplasm conservation of valuable and endangered medicinal plants (Ajithkumar and Seeni 1998;Tiwari et al 2000;Mokhtari 2016). In vitro culture studies include aspects such as direct organogenesis.…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of plant growth regulators and explants on direct shoot regeneration of Viola odorata

Mokhtari¹,

Zarei²,

Samsamzadeh³

et al. 2016

bta

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The aim of the study was to develop a protocol for in vitro shoot regeneration based on a solid Murashige and Skoog (MS) basal medium supplemented with various concentrations of plant growth regulators (PGRs) like Indole-3-butyric acid (IBA), kinetin (KIN) or benzylaminopurine (BAP) using leaf, petiole, root, and crown disk segments of a valuable medicinal plant Viola odorata. The best percentage of direct shoot regeneration (67.50%) and the maximum number of micro-shoots (9.50) were obtained with crown explants on MS medium containing IBA (0.5 mg A l ). The best elongation (5.25 cm) of micro-shoots was observed in crown explants on a medium containing 1 mg A l !1 gibberellic acid (GA3) followed by GA3 at 0.5 mg A l !1 and similar explants. Irrespective of the concentrations of GA3, the minimum length of shoots was observed in leaf explants. A half-strength MS medium containing activated charcoal and supplemented with 1.5 mg A l !1 IBA resulted in the best rooting (78%) of elongated shoots. All acclimatized plantlets survived and showed a normal growth.The study reports a simple and applicable protocol for micropropagation of V. odorata through direct organogenesis.

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“…D) which was significantly different from other treatments. Use of auxins singly or in combination for rooting was also reported by different authors (Sahoo and Chand, 1998;Ajithkumar and Seeni, 1998;Rai, 2002;Sivakumar and Krishnamurthy, 2000;Hassan and Roy, 2004;Baksha et al, 2007;Hassan, 2008).…”

Section: Resultsmentioning

confidence: 98%

In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

Huq

Akter

Islam

et al. 2012

Bangladesh J. Sci. Ind. Res.

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An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 91.66%, when nodal explants were cultured on MS+1.0 mg/1 BAP. The maximum number of shoots (4.8) per explant was observed in MS + 1.0 mg/1 BAP + 0.2 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration and multiplication. The best response towards root induction was achieved on half MS medium supplemented with 0.4 mg/1 NAA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Immediately after transplantation the plantlets were covered with polythene bag to prevent desiccation. After acclimatization the plantlets were successfully transferred to the garden soil and the percentage of survivability in such condition was 90%.

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Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree

Cited by 105 publications

References 11 publications

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Interactive effects of plant growth regulators and explants on direct shoot regeneration of Viola odorata

In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

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