Rapid colorimetric assay for the quantification of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)

Ohno, Miho; Abe, Tsutomu

doi:10.1016/0022-1759(91)90327-c

Cited by 243 publications

(130 citation statements)

References 15 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…To further determine the functional consequences of our v-Src constructs we performed a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay (SI Fig. 10) (25). SYF cells were transfected with 1 and 6a or 6b, empty vector, or a plasmid containing v-Src.…”

Section: Surf Is a General Methods For Controlling Protein Levelsmentioning

confidence: 99%

Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt

Pratt

Schwartz²,

Muir³

2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Section: Surf Is a General Methods For Controlling Protein Levelsmentioning

confidence: 99%

Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt

Pratt

Schwartz²,

Muir³

2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

“…Culture medium with corresponding concentrations of investigated compounds, but without cells, was used as blank. The cultures were incubated for 72 h, and the effects of the investigated compounds on cancer cell survival were determined using the microculture tetrazolium test (MTT), according to Mosmann (36) with modification by Ohno and Abe (37). Briefly, 20 mL of MTT solution (5 mg/mL of phosphate-buffered saline, PBS) was added to each well.…”

Section: Determination Of Cell Survivalmentioning

confidence: 99%

Antiproliferative Activity of Gold(III) Complexes with Esters of Cyclohexyl-Functionalized Ethylenediamine-N,N’-Diacetate

Pantelić

Stanojković

Zmejkovski

et al. 2017

Serbian Journal of Experimental and Clinical Research

View full text Add to dashboard Cite

Six gold(III) complexes with esters of cyclohexyl-functionalized ethylenediamine-N,N'-diacetate, general formula [AuCl 2 {(S,S)-R 2 eddch}]PF 6 , [(S,S)-eddch = (S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate, R = Me, Et,[1][2][3][4][5][6], respectively], were tested against cancer cell lines such as human melanoma Fem-x, human colon carcinoma LS174T and non-small cell lung carcinoma A549 as well as a non-cancerous human embryonic lung fi broblasts [3][4] SAŽETAK Šest kompleksa zlata(III) sa cikloheksil-funkcionalizovanim estrima etilendiamin-N,N'-diacetata, opšte formule [AuCl 2 {(S,S)-R 2 eddch}]PF 6 , ((S,S)-eddch = (S,S)-etilendiamin-N,N'-di-2-(3-cicloheksil)propanoat, R = Me, Et, n-Pr, n-Bu, i-Bu, i-Am, 1-6), ispitivano je na humanim ćelijskim linijama malignog melanoma Fem-x, karcinoma debelog creva LS174T, karcinoma pluća A549 kao i normalnim ćeli-jama MRC-5 (fetalni plućni fi broblast) korišćenjem

show abstract

“…Cell survival was determined by MTT test according to the method of Mosmann and modified by Ohno and Abe, 72 h after addition of the compounds 31,32 . Briefly, 20 mL of MTT solution (5 mg/mL in phosphate buffered saline) was added to each well.…”

Section: Antiproliferative Activitymentioning

confidence: 99%

“…Cell survival was determined by MTT test, after 72 h of exposure to compounds 31,32 . IC 50 values (Table 1) are shown in molar concentrations, as the mean ± SD, determined from three independent measurements.…”

Section: Antiproliferative Activitymentioning

confidence: 99%

Antiproliferative and antibacterial activity of some glutarimide derivatives

Popović‐Djordjević¹,

Klaus

Žižak

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

Antiproliferative and antibacterial activities of nine glutarimide derivatives (1-9) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC 50 ¼ 9-27 mM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds 1, 2, 4, 6-8 and 9 inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound 9 (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against Bacillus cereus (MIC 0.625 mg/mL; 1.97 Â 10 À3 mol/L). Distinction between more and less active/ inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields.

show abstract

Rapid colorimetric assay for the quantification of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)

Cited by 243 publications

References 15 publications

Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt

Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt

Antiproliferative Activity of Gold(III) Complexes with Esters of Cyclohexyl-Functionalized Ethylenediamine-N,N’-Diacetate

Antiproliferative and antibacterial activity of some glutarimide derivatives

Contact Info

Product

Resources

About