Rapid communication: polymerase chain reaction-restriction fragment length polymorphism in the bovine growth hormone gene

Mm, Unanian; Sk, DeNise; Hm, Zhang; Rl, Ax

doi:10.2527/1994.7282203x

Cited by 18 publications

(14 citation statements)

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“…Three missense nucleotide substitutions were detected in exon 5 at codon 127 Val/Leu (Lucy et al, 1993), 172 Thr/ Met (Chikuni et al, 1994) and 112 Arg/Asp (Yoon et al, 2003). Other polymorphic sites were also reported in the promoter region (Rodrigues et al, 1998;Ferraz et al, 2006), 3' flanking region (Unanian et al, 1994) and intron 3 of the GH1 gene (Zhang et al, 1993). POU1F1, a pituitary-specific transcription factor is responsible for pituitary development and hormone expression levels in mammals.…”

Section: Introductionmentioning

confidence: 86%

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Han

Cho

et al. 2010

Genes Genom

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Growth hormone and pituitary specific transcription factor-1 are expressed in the pituitary gland and play critical roles in the development and growth of mammals. Genetic polymorphisms of GH1 and POU1F1 genes were investigated using PCR-RFLP and tested for statistical associations with meat quality traits in Hanwoo cattle. Three genotypes were found for each gene in a population of Hanwoo steers reared for beef production. No meat quality traits were significantly associated with the GH1 genotypes. Animals with the GH1 Leu/Leu genotype had heavier weights and higher marbling scores, but statistical significance was not detected. Backfat thickness was associated with POU1F1 genotypes. Backfat of POU1F1 G/G homozygotes was thicker than that of other genotypes (p ＜ 0.05). Other carcass traits, including weight at slaughter, carcass weight, carcass yield ratio, eye muscle area, marbling score, meat color, and fat color were statistically insignificant in POU1F1 genotypes (p ＞ 0.05). These findings suggest that the POU1F1 G/G genotype may contribute to improving body weights and backfat in Hanwoo steers using sire-based predictions.

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Section: Introductionmentioning

confidence: 86%

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Han

Cho

et al. 2010

Genes Genom

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“…O DNA assim obtido foi amplificado por meio da reação em cadeia da polimerase (PCR), de acordo com ZHANG et al (1992de acordo com ZHANG et al ( , 1993, UNANIAN et al (1994) e LUCY et al (1991. Para cada reação, foram utilizados 100 ng DNA, 1 x Taq Buffer (100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl 2 , 0,01% gelatina e 1% Triton X-100; pH 9,0), 1,5 mM MgCl 2 , 200 µM dNTP, 2 U Taq DNA polimerase, 0,8 mM de cada oligonucleotideo, num volume final de 50 µL.…”

Section: Methodsunclassified

“…Para a digestão com a endonuclease Hae III, amplificou-se um fragmento de 441 pb contendo o exon V e a região flanqueadora 3' (UNANIAN et al, 1994) e, para Alu I, um fragmento de 427 pb constituído de 55 pb do exon IV (3'região), 273 pb do intron 4 e 99 pb do exon V (5'região) (ZHANG et al, 1992) do gene do homônimo de crescimento, conforme a seqüência descrita por GORDON et al (1983).…”

Section: Methodsunclassified

Associação do polimorfismo do gene do hormônio de crescimento com a caraterística peso em bovinos da raça Nelore

et al. 2000

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RESUMO -O polimorfismo do gene do hormônio de crescimento bovino (bGH) foi estudado em 211 bovinos machos da raça Nelore, puros de origem (PO), a fim de observar as freqüências genotípicas e alélicas, e a possível associação com a característica peso. Para alcançar este objetivo, foram considerados os pesos ao nascer, à desmama e mensais dos 10 aos 16 meses de idade, e calculados os ganhos de peso do nascimento à desmama e da desmama aos 16 meses. Foi coletado sangue para extração do DNA e análise dos sítios polimórficos (RFLP) oriundos da digestão com as endonucleases Msp I (bGH/Msp I, 891 pb), Hae III (bGH/Hae III, 441 pb) e Alu I (bGH/Alu I, 427 pb). Para cada polimorfismo foram encontrados dois alelos, ocorrendo predominância dos alelos D, F e A, respectivamente. Foi observado o efeito do genótipo AA do bGH/Alu I sobre o ganho de peso da desmama até 15 meses de idade e do genótipo DD do bGH/Msp I sobre os ganhos de peso da desmama aos 14 e 15 meses. Os resultados sugerem que os polimorfismos bGH/Alu I e bGH/Msp I constituem marcadores, em potencial, da característica ganho de peso em animais jovens.Palavras-chave: bovinos, gene do hormônio de crescimento, pesos, raça Nelore Associations between Growth Hormone Gene Polymorphism and Weight Traits in Nellore BovinesABSTRACT -The polymorphism of the growth hormone gene (bGH) was studied in 211 Nellore pure breed bovine males to estimate the genotype and allelic frequencies and possible associations of the gene with weight traits. For this study, body weight data were collected at birth, weaning and monthly from 10 to 16 months of age. Additionally, weight gains from birth to weaning and weaning to 16 months of age were calculated. DNA was extracted from blood samples and the animals genotyped for Msp I (bGH/Msp I, 891 bp), Hae III (bGH/Hae III, 441 bp) and Alu I (bGH/Alu I, 427 bp) polymorphic sites. Every polymorphism presented two alleles. The predominant alleles were D, F and A, respectively. The effect of the bGH/Alu I AA genotype was observed on the weight gain from weaning to 15 months of age, and of the bGH/Msp I DD genotype from weaning to 14 and 15 months. The results suggest that bGH/Alu I and bGH/Msp I polymorphisms may be potential markers for the weight gain trait in young bulls.

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“…The additional polymorphic site in the somatotropin gene (point mutation C→T in the codon 172) was found in the Japanese cattle and consequently threonine was changed to methionine in a protein chain (CHIKUNI et al, 1994). Furthermore, UNANIAN et al (1994) detected a polymorphic site in the 3'-region of the GH gene, probably in position 2637. The studies on the influence of the Leu/Val polymorphism on production traits are quite advanced but the results obtained by various researchers are not always corresponding.…”

Section: Introductionmentioning

confidence: 99%

Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

Dybus

2002

Arch. Anim. Breed.

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SummaryAssociations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production traits of Black-and-White cattle with different proportional share of Holstein Friesian genes were analysed. A total of 1086 cows were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles were as follows: 0.653 for LL genotype, 0.324 for LV and 0.023 for VV, and 0.815 for GH L and 0.185 for GH V . Associations between Leu/Val polymorphism and milk production traits of cows were found only in first lactation. Cows with LL genotype had higher milk, fat and protein yield compared to LV individuals (P ≤ 0.01).

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Rapid communication: polymerase chain reaction-restriction fragment length polymorphism in the bovine growth hormone gene

Cited by 18 publications

References 0 publications

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Associação do polimorfismo do gene do hormônio de crescimento com a caraterística peso em bovinos da raça Nelore

Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

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Rapid communication: polymerase chain reaction-restriction fragment length polymorphism in the bovine growth hormone gene

Cited by 18 publications

References 0 publications

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Effects of POU1F1 and GH1 genotypes on carcass traits in Hanwoo cattle

Associação do polimorfismo do gene do hormônio de crescimento com a caraterística peso em bovinos da raça Nelore

Associations between &lt;i&gt;Leu/Val&lt;/i&gt; polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

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Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle