Rapid Compound Integrity Assessment for High-Throughput Screening Hit Triaging

Shou, Wilson Z.; Gerritz, Samuel W.; Harden, David G.; Lawrence, R. Michael; Chase, Peter; Chin, Jefferson; Surti, Neha; Lippy, Jonathan; Weller, Harold N.; Nielsen, Julia; Weerakoon, Darshani

doi:10.1177/2472555220919097

Cited by 4 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…W skriningu pierwszorzędowym testuje się całą wybraną bibliotekę związków chemicznych zazwyczaj w jednym powtórzeniu, w tych samych warunkach przy wykorzystaniu zoptymalizowanej wcześniej procedury i automatyzacji. Selekcje potencjalnie aktywnych związków dokonuje się poprzez przekroczenie wyznaczonej granicznej wartości % aktywności badanego związku, która różnicuje populacje potencjalnie aktywnych i nieaktywnych substancji [48].…”

Section: Etapy Skrininguunclassified

Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym

Karczewska,

Pyc,

Żukowski

et al. 2024

Postepy Biochem

View full text Add to dashboard Cite

With the development of medicine and the aging population, the demand for more effective therapies is escalating. A tool that facilitates the discovery and introduction of new therapeutic drugs is High-Throughput Screening (HTS). These tests, consisting of a wide set of various assays, allow testing hundreds of thousands of compounds in a short period of time. The aim is to accurately identify active compounds that could become potential therapeutic candidates in the pharmaceutical industry. HTS is the first step in the quest for potential drugs, therefore it is one of the crucial tests that determine whether a particular drug candidate will be discovered. In this review, different stages of high-throughput screening studies will be described, as well as methods utilized in these studies. The necessary steps in the optimization of these tests, selection of equipment, automation and key quality control parameters for reliably performed screening will also be presented.

show abstract

Section: Etapy Skrininguunclassified

Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym

Karczewska,

Pyc,

Żukowski

et al. 2024

Postepy Biochem

View full text Add to dashboard Cite

show abstract

“…Published work covering chemical instability in DMSO has been limited, 4–8 and attempts to build a predictive model have been only partially successful. 9,10 This is not surprising, considering the range of chemistries involved.…”

Section: Introductionmentioning

confidence: 99%

Maintaining a High-Quality Screening Collection: The GSK Experience

et al. 2021

View full text Add to dashboard Cite

“…The next two articles reinforce the importance of compound quality and properties when developing small-molecule screening cascades. Wilson Shou et al 23 defined how proper high-throughput analysis ensured maintenance of compound integrity and determined the highest-quality molecules to be followed up for HTS. In their article, Gareth Davies et al 24 shared a high-throughput approach to decipher the mechanism of action of compounds at enzyme targets.…”

mentioning

confidence: 99%