Rapid Destruction of Bacteria in Commonly Used Diluents and Its Elimination

Straka, Robert P.; Stokes, J. L.

doi:10.1128/aem.5.1.21-25.1957

Cited by 113 publications

(39 citation statements)

References 6 publications

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“…The sample was weighed and macerated in 45 ml of 0.1 yo peptone medium (Straka & Stokes, 1957) using an Ato-mix blender (Measuring & Scientific Equipment Ltd., London).…”

Section: Methodsmentioning

confidence: 99%

Microbial Changes Leading to the Spoilage of Hung Pheasants, with Special Reference to the Clostridia

Mead

Chamberlain

Borland

1973

Journal of Applied Bacteriology

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ABSTRACT. A spoilage problem affecting hung pheasants is described in which carcasses showed rapid greening and deterioration during hanging. No micro‐organisms were detected in samples of muscle tissue taken from abnormal carcasses but clostridia and often coli‐aerogenes bacteria were unusually abundant in the duodenum and in the remainder of the small intestine. The clostridia were mainly Clostridium bifermentans/sordellii or strains resembling Cl. welchii and these organisms outgrew all other bacteria in the duodenum under suitable hanging conditions. When grown at low temperatures strains of clostridia and Escherichia coli from pheasants produced large amounts of H2S which is concerned in the greening process. Some other properties of the clostridia are described and the significance of the spoilage problem is discussed.

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“…The sample was weighed and macerated in 45 ml of 0.1 yo peptone medium (Straka & Stokes, 1957) using an Ato-mix blender (Measuring & Scientific Equipment Ltd., London).…”

Section: Methodsmentioning

confidence: 99%

Microbial Changes Leading to the Spoilage of Hung Pheasants, with Special Reference to the Clostridia

Mead

Chamberlain

Borland

1973

Journal of Applied Bacteriology

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“…Potato tubers were cut and shaken vigorously for 30 min at 19-20*^C in 200 ml sterile distilled water containing 0.1 % proteose peptone (Difco) to protect bacteria from adverse dilution effects (STRAKA and STOKES 1957). Appropriate dilutions were plated on modified King's medium B (KING et al 1954) further referred to as KB, supplemented with cycloheximide (100 ppm), ampicillin (30 ppm) and chloramphenicol (12.5 ppm) for selective isolation of pseudomonads (medium KB+).…”

Section: Isolation Of Fluorescent Pseudomonadsmentioning

confidence: 99%

Selection of Antagonistic Fluorescent Pseudomonas spp. and their Root Colonization and Persistence following Treatment of Seed Potatoes

Geels¹,

Schippers²

1983

Journal of Phytopathology

199

105

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One hundred and twelve fluorescent Pseudomonas isolates from potato periderm were screened for antagonistic activity in vitro against 5 pathogenic and saprophytic fungi, 5 Gram‐positive bacteria including Streptomyces scabies and 4 Gram‐negative bacteria including Erwinia car. var. carotovora and Erwinia car. var. atroseptica. Fifty‐seven percent of the isolates showed wide spectrum inhibitory activity, mostly due to the production of fluorescent siderophores. Only 4 % of the isolates were very strong antagonists. By adding iron (Fe3+) to the test medium, the antagonistic activity drastically diminished but revealed some strong antagonists of which the antagonism was not affected. Apparently growth‐inhibiting substances other than siderophores were involved. Antibiotic‐resistant mutants of the antagonists were used to establish that the bacteria on the seed tubers migrated to the roots under different conditions. Drying of the treated potatoes gradually diminished the number of viable cells, but subsequent planting in moist soil led to substantial increases in the first three days. Initially high numbers of the antibiotic‐resistant antagonists on the roots tended to drop quickly in time, probably due to loss of resistance to the antibiotics in the selective medium.

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“…Sites of 25 cm2 delineated by a sterilized steel template were swabbed with cotton-tipped applicator sticks which had been moistened in diluent. The cotton tips of the applicator sticks were then placed in Quarter Strength Ringer Diluent (Oxoid BR52) containing 0.1% Peptone (Oxoid L37; Straka & Stokes 1957) or in Quarter Strength Ringer Thiosulphate Diluent (Oxoid BR48) containing 0.1% peptone if the carcase had received a washing treatment which incorporated chlorine. Swabs from three sites on the front of the carcase, shown in Fig.…”

Section: Bucterio Logical Samplingmentioning

confidence: 99%

The Effect of Temperature, Pressure and Chlorine Concentration of Spray Washing Water on Numbers of Bacteria on Lamb Carcases

Kelly¹,

Dempster²,

McLoughlin³

1981

Journal of Applied Bacteriology

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Combined analysis of three experiments showed that when lamb carcases with initial bacterial numbers of between logi103.29 and 4.22/cm2 were spray washed, statistically significant reductions in bacterial numbers of log10O.5 were obtained when the spray wash water temperature was > 57°C, and reductions of log101.0 were obtained when the temperature was ≥ 80°C. Reductions at all temperatures were enhanced by log100.66 when the water contained 30 µg/ml chlorine, but increasing the concentration to 450 µg/ml reduced bacterial numbers only by a further log100–29. At highly contaminated sites increasing the duration of spraying from 30 to 120 s significantly increased the reductions obtained when water containing added chlorine was used. Reductions in bacterial numbers after spray washing with pressures of 3.5, 5.6. 7.7 kg/cm2 were not significantly different.

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Rapid Destruction of Bacteria in Commonly Used Diluents and Its Elimination

Cited by 113 publications

References 6 publications

Microbial Changes Leading to the Spoilage of Hung Pheasants, with Special Reference to the Clostridia

Microbial Changes Leading to the Spoilage of Hung Pheasants, with Special Reference to the Clostridia

Selection of Antagonistic Fluorescent Pseudomonas spp. and their Root Colonization and Persistence following Treatment of Seed Potatoes

The Effect of Temperature, Pressure and Chlorine Concentration of Spray Washing Water on Numbers of Bacteria on Lamb Carcases

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