Rapid detection and quantification of Enterovirus 71 by a portable surface plasmon resonance biosensor

Prabowo, Briliant Adhi; Wang, Chia Hui; Secario, K.; Ou, P.-T.; Alom, Azharul; Liu, Jia-Jung; Liu, Kou−Chen

doi:10.1016/j.bios.2017.01.043

Cited by 57 publications

(37 citation statements)

References 44 publications

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“…The Essential Macleod simulation tool (Thin Film Center Inc., Tucson, AZ, USA) is used to simulate the SPR reflectivity profile. In our group, we developed the direct, rapid, and simple labor intensive for viral detection using the organic light-emitting diode (OLED) based portable surface plasmon resonance (SPR) sensor for several years [7], as the platform is depicted in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Novel Four Layer Metal Sensing in Portable SPR Sensor Platform for Viral Particles Quantification

Prabowo

Alom

Pal

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Label-free, direct, rapid and real-time quantification method for human enterovirus 71 (EV71) using surface plasmon resonance (SPR) sensor is presented in this study. The performance of the four layer sensor chip was compared with the conventional Au monolayer sensor chip. Four layer sensor chip gave optimum resonance wavelength around 610 nm, which was same with the OLED light source peak wavelength and gave better quality factor (Q = DIP/FWHM). The result showed that the structure with 3 nm Al, 10 nm Au, 20 nm Ag, 10 nm Au layer sensor chip gave better limit of detection than the 3 nm Cr, and 47 nm Au layer sensor chip. The detection limit of direct quantification of EV71 particles is improved to 43 vp/mL of EV71 in DMEM medium. The results proved that the SPR biosensor with four layer sensor chip structure demonstrated great performance in the quantification of EV71 virus species.

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Section: Methodsmentioning

confidence: 99%

Novel Four Layer Metal Sensing in Portable SPR Sensor Platform for Viral Particles Quantification

Prabowo

Alom

Pal

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

Self Cite

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“…The SPR system can be miniaturized for wider application in the field and laboratory. Prabowo et al (2017) have developed such portable detection system for label-free, rapid quantification of human Enterovirus 71 (EV71). These are small RNA viruses belonging to picornviridae family with a very high mutation rate (Fig.…”

Section: Detection and Quantification Of Enterovirus 71mentioning

confidence: 99%

“…By combining the SPR assay with the viral plaque assay, the assay time was reduced from days to minutes with a detection limit of approximately 67 virus particles of EV71 per milliliter (vp/ml) in Dulbecco's modified Eagle's medium. This platform could be further improved by incorporating a highly sensitive, real-time, optical biosensor-based assay Prabowo et al, 2017).…”

Section: Detection and Quantification Of Enterovirus 71mentioning

confidence: 99%

Surface Plasmon Resonance: A Boon for Viral Diagnostics

Singh

2017

Reference Module in Life Sciences

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“…EV71 is the most common pathogens of hand, foot and mouth disease (HFMD) in infants and children under 6 years old, which usually leads to bad clinical consequence and even death in severe cases [7]. To date, the pathogenesis of EV71 has not been clarified and no vaccine or specific antiviral agent is available for treating EV71 infection [8]. Thus, the development of non-toxic and effective antiviral agents against EV71 is imminently needed [9].…”

Section: Introductionmentioning

confidence: 99%

Functionalized selenium nanoparticles enhance the anti-EV71 activity of oseltamivir in human astrocytoma cell model

Zhong

Xia

Hua

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Enterovirus 71 (EV71) which commonly caused the hand-foot-mouth disease (HFMD) has become one of public health challenges worldwide. However, no effective vaccines or drugs for this disease has been developed. Thus, there is an urgent need to find a new strategy for treating the EV71 infection. Oseltamivir (OT) is an effective antiviral agent, but continuous use of oseltamivir leads to a diminished therapeutic effect in the clinic. In order to improve the antiviral activity of oseltamivir, oseltamivir was loaded onto surfaces of selenium nanoparticles (SeNPs) to fabricate a functionalized antiviral nanoparticles SeNPs@OT. The size of SeNPs@OT was tested by TEM and dynamic light scattering. The chemical structure and elemental composition of SeNPs@OT were analyzed by FT-IR and EDX, respectively. SeNPs@OT exhibited good stability and effective drug release in serum and PBS. SeNPs@OT efficiently entered into human astrocyte U251 cells (host cells) via clathrin-associated endocytosis and inhibited EV71 proliferation, which could protect EV71-infected U251 cells from apoptosis through mitochondrial pathway. Furthermore, SeNPs@OT inhibited EV71 activity probably by reducing the generation of reactive oxygen species in EV71-infected U251 cells. Interestingly, SeNPs obviously enhanced antiviral activity of oseltamivir in the anti-EV71 cell model. Taken together, SeNPs@OT is a promising antiviral drug candidate for EV71 infection.

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Rapid detection and quantification of Enterovirus 71 by a portable surface plasmon resonance biosensor

Cited by 57 publications

References 44 publications

Novel Four Layer Metal Sensing in Portable SPR Sensor Platform for Viral Particles Quantification

Novel Four Layer Metal Sensing in Portable SPR Sensor Platform for Viral Particles Quantification

Surface Plasmon Resonance: A Boon for Viral Diagnostics

Functionalized selenium nanoparticles enhance the anti-EV71 activity of oseltamivir in human astrocytoma cell model

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