Rapid detection of group B streptococcal antigen from vaginal specimens using a new Optical ImmunoAssay technique

Park, Choong H.; Ruprai, Davinder; Vandel, Nancy M.; Hixon, Deborah L.; Mecklenburg, Fred E.

doi:10.1016/0732-8893(96)00013-2

Cited by 19 publications

(18 citation statements)

References 7 publications

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“…The most important reason for this variability may be the extent to which the subjects were colonized. The sensitivity of the OIA has been higher (61 to 100%) when compared with third-and fourth-quadrant positive blood agar cultures (2,5,7,(12)(13)(14). Our finding was within this range (68.4%).…”

supporting

confidence: 69%

“…The specificity was uniformly high, 85 to 98%, similar to the 98.4% in our study. Conversely, previously reported test sensitivities varied widely but were generally higher (37 to 81%) (2,5,7,(12)(13)(14)(17)(18)(19) than the value we found (26.8%). The most important reason for this variability may be the extent to which the subjects were colonized.…”

contrasting

confidence: 56%

“…Unfortunately, most rapid GBS tests have poor sensitivity (7,13). The Strep B optical immunoassay (OIA; ThermoBiostar, Inc., Louisville, Colo.) is a rapid method which may have higher sensitivity for dense GBS colonization (14). We evaluated the performance of this test in an international, multicenter study.…”

mentioning

confidence: 99%

“…For now, culture of specimens from the rectum and lower vagina remain the gold standard for detecting GBS colonization in pregnant women (8). a The GBS colonization levels were defined as follows: light, isolation of GBS from Lim broth alone; heavy, isolation of GBS from either urine or direct plating on blood agar; dense, growth in the third or fourth quadrant streaked on the blood agar plate (14).…”

mentioning

confidence: 99%

“…Light colonization was defined as isolation of GBS from Lim broth alone. Dense GBS colonization was defined as growth in the third or fourth quadrant streaked on the blood agar plate (14).…”

mentioning

confidence: 99%

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Infections in International Pregnancy Study: Performance of the Optical Immunoassay Test for Detection of Group B Streptococcus

et al. 2003

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We evaluated the Strep B optical immunoassay (OIA; ThermoBiostar, Inc.) for detecting light and heavy group B streptococcus colonization in 1,306 pregnant women. The women were examined at 20 to 32 weeks gestation and were from six countries. Compared to culture, the sensitivity and specificity of OIA were 13.3 and 98.4%, respectively, for light colonization and 41.5 and 97.7%, respectively, for heavy colonization.Heavy colonization with group B streptococcus (GBS) may place women at high risk of delivering preterm, low-birthweight infants (10,11,15). Earlier recognition of such high-risk women would be useful for intervention strategies. Detection of heavy colonization has traditionally relied on culture methods, which may not be feasible in some health care settings and require 1 to 3 days before results are available. Unfortunately, most rapid GBS tests have poor sensitivity (7, 13). The Strep B optical immunoassay (OIA; ThermoBiostar, Inc., Louisville, Colo.) is a rapid method which may have higher sensitivity for dense GBS colonization (14). We evaluated the performance of this test in an international, multicenter study.We conducted the study at institutions participating in the Global Network for Perinatal and Reproductive Health (http: //www.tju.edu/obgyn/rih/global.cfm). Centers were located in Khon Kaen and Bangkok, Thailand; Manila, The Philippines; Harare, Zimbabwe; Yangon, Myanmar; Dublin, Ireland; and Philadelphia, Pennsylvania. Participants were enrolled in the study between June 1999 and May 2001. Women attending prenatal care clinics and at 20 to 32 weeks gestation were eligible. Exclusion criteria included vaginitis symptoms, fever, vaginal bleeding, multiple pregnancy, use of antibiotics in the past 2 weeks, and active labor. Informed consent was obtained. The study was approved by institutional review boards at all study centers and supporting institutions.At enrollment, study personnel conducted an interview and collected specimens. A sample for GBS rapid testing was collected from the cervix and lower vagina using a ThermoBiostar swab. OIAs were read by study site laboratory personnel and by the manufacturer's laboratory personnel. OIA kits and swabs were supplied by ThermoBiostar.Methods for GBS culture and quantitation replicated those used in the Vaginal Infections and Prematurity Study (16). After collection of the OIA specimen, a Dacron swab (Copan swab) was used to collect a specimen from the cervix and lower vagina. Samples were streaked onto blood agar plates using the four-quadrant method and then placed into Lim broth (ToddHewitt broth containing selective antimicrobial agents). A clean catch urine specimen was collected for GBS culture on blood agar. Suspect colonies showing ␤-hemolysis were identified as GBS using latex agglutination. Heavy colonization was defined as isolation of GBS from either urine or direct plating on blood agar. Light colonization was defined as isolation of GBS from Lim broth alone. Dense GBS colonization was defined as growth in the third or fourth quadr...

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supporting

confidence: 69%

contrasting

confidence: 56%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Infections in International Pregnancy Study: Performance of the Optical Immunoassay Test for Detection of Group B Streptococcus

et al. 2003

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Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Nguyen

Gauthier

Myles

et al. 1998

J. Matern. Fetal Med.

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The aim of this study was to compare the diagnostic accuracy of an optical immunoassay (STREP B OIA, Biostar) to direct plating and broth‐enhanced culture for the detection of group B streptococcus (GBS) colonization of the lower genital tract in pregnant women. GBS cultures from the lower genital tract were obtained in a prospective fashion using a dual swab transport system from patients with risk factors for perinatal GBS infection. One swab was used to inoculate a trypticase soy agar plate with 5% sheep blood (TSA) and then placed in Lim broth. The other swab was used to perform the Strep B OIA. Growth of GBS by either direct plating or broth‐enhanced culture was used as the gold standard for determining GBS colonization. Of the 524 women in the study, 90 women had positive cultures (either TSA or Lim broth). The sensitivity, specificity, positive predictive value, and negative predictive value of the Strep B OIA were 47% (42/90), 96% (416/434), 70% (42/60), 90% (416/464). The sensitivity, specificity, positive predictive value, and negative predictive value of the TSA were 61% (55/90), 100% (434/434), 100% (55/55), 93% (434/469). The sensitivity, specificity, positive predictive value, and negative predictive value of Lim broth were 97% (87/90), 100% (434/434), 100% (87/87), and 97% (434/437). The sensitivity of the Strep B OIA to detect light GBS colonization and heavy GBS colonization, as determined by the TSA, was 53% (19/36) and 90% (17/19), respectively. The Strep B OIA and direct agar plate culture appear to be of limited clinical value due to their poor sensitivities. This study also demonstrates the need to use a selective medium such as Lim broth when assessing for GBS colonization of the lower genital tract. J. Matern.‐Fetal Med. 7:172–176, 1998. © 1998 Wiley‐Liss, Inc.

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Spot tests: past and present

Doménech‐Carbó

2021

ChemTexts

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Graphic abstract Microchemistry, i.e., the chemistry performed at the scale of a microgram or less, has its roots in the late eighteenth and early nineteenth centuries. In the first half of the twentieth century a wide range of spot tests have been developed. For didactic reasons, they are still part of the curriculum of chemistry students. However, they are even highly important for applied analyses in conservation of cultural heritage, food science, forensic science, clinical and pharmacological sciences, geochemistry, and environmental sciences. Modern pregnancy tests, virus tests, etc. are the most recent examples of sophisticated spot tests. The present ChemTexts contribution aims to provide an overview of the past and present of this analytical methodology.

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Rapid detection of group B streptococcal antigen from vaginal specimens using a new Optical ImmunoAssay technique

Cited by 19 publications

References 7 publications

Infections in International Pregnancy Study: Performance of the Optical Immunoassay Test for Detection of Group B Streptococcus

Infections in International Pregnancy Study: Performance of the Optical Immunoassay Test for Detection of Group B Streptococcus

Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Spot tests: past and present

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