Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor

Abstract

Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids. The sensor successfully achieved the detection of loop-mediated isothermal amplification for the hepatitis virus in this work. The sensor comprised a nanodisc array and Bst polymerases conjugated on the rough surface of a nanodisc. The rough surface of the nanodisc provided plasmonic hot spots to enhance the fluorescence signal. The virus DNA was detected by conducting a modified loop-mediated isothermal amplification with fluorescence resonance energy transfer reporter conjugated primers on the sensor. The modified isothermal amplification improved the signal contrast and detection time compared to the original assay. By integrating the modified amplification assay and plasmonic enhancement sensor, we achieved rapid detection of the hepatitis virus. Nucleic acid with a concentration of 10−3 to 10−4 mg/mL was detected within a few minutes by our design. Our digitizing plasmonic nanoarray biosensor also showed 20–30 min earlier detection compared to conventional loop-mediated isothermal amplification sensors.

Recent Development in Plasmonic Nanobiosensors for Viral DNA/RNA Biomarkers

“…In this study, DNA could be measured by ten femtomolar levels without a target amplification step. Wei et al showed the digitalizing biosensor for the rapid detection of viral DNA using loop-mediated isothermal amplification (LAMP) and Au nanoarray [ 55 ]. The rough Au sensing surface could provide the hot spot to generate the MEF.…”

“… Viral DNA-target SERS and electrochemical biosensors using plasmonic nanomaterials: ( a ) Schematic diagram of the SERS-active viral DNAs detection platform using rough-faced Au nanostructure with GO and Raman dye-functionalized AuNPs. This figure is reproduced from [ 55 ] (© 2022 American Chemical Society); ( b ) Schematics of the SERS-based sensitive DNA detection using hierarchic interfacial Au nanocube assembly. This figure is reproduced from [ 58 ] (© 2022 American Chemical Society); ( c ) Plasmonic AuNPs-assisted colorimetric biosensor for detection of HCV DNA.…”

The Current Status and Future Promise of SPR Biosensors

“…In this Special Issue, Wei et al [ 22 ] investigated the rapid detection of virus nucleic acid via isothermal amplification on a plasmonic-enhanced digitizing biosensor. The development of rapid and accurate pathogen screening technology is of great significance for the early diagnosis and prevention of infectious diseases.…”