Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Saharan, Pooja; Khatri, Poonam; Dingolia, S.; Duhan, Joginder Singh; Gahlawat, Suresh Kumar

doi:10.1007/978-81-322-1683-4_21

Cited by 15 publications

(11 citation statements)

References 157 publications

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“…LAMP has the advantages of reaction simplicityand detection sensitivitycompared with PCR.LAMP is an innovative, new generation, geneamplification technique that can amplify the targetsequence with a high degree of sensitivity and specificityunder isothermal condition. 34 As detection procedure is easy,i.e., on real-time nucleic acid amplification, this assaycan be used as point of care diagnostic toolfor infectious human or animal disease. Thus, it will provide agreat platform for quick and accurate identification ofdifferent pathogens in medical as well as veterinaryfield.…”

Section: Resultsmentioning

confidence: 99%

“…26 LAMP had been developed for many plant viruses such as Wheat streak mosaic virus (WSMV) 27 , Wheat yellow mosaic virus (WYMV), Japanese soil-borne mosaic virus (JSBWMV) and Chinese wheat mosaic virus (CWMV) 28 , Yellow head virus (YHV) 29 , bovine viral diarrhea viral (BVDV) 30 , Potato virus Y (PVY) 31 , tomato torrado virus (ToTV) 32 , Sugarcane mosaic virus (SCMV) 33 , tomato spotted wilt virus, necrotic spots virus, and chrysanthemum stunt virus, tobacco mosaic virus, banana streak virus, cauliflower mosaic virus, yellow mosaic virus, potato virus Y, potato spindle tuber viroid, peach latent mosaic viroid, wheat yellow mosaic virus. 34…”

Section: Detection Of Plant Virusesmentioning

confidence: 99%

“…Equine arteritis virus (EAV), porcine reproductive and respiratory syndrome virus (PRRSV), lactate dehydrogenase elevating virus (LDV) of mice, and simian hemorrhagic fever virus (SHFV) all belong to the Arteriviridae family of viruses. 34 In addition, it has been used to determine sex of asparagus, genetically modified organisms (GMOs), and Phytoplasmas. 42…”

Section: Detection Of Animal Virusesmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification (LAMP) for Detection of Various Organisms: A Review

Akram¹

2017

Bangladesh J Med Microbiol

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Disease diagnosis is important for implementation of proper therapeutic and prophylactic measures. Traditionally, disease diagnosis was depends upon isolation and identification of the causative organisms. This was followed by serology and after that molecular method. Molecular tests are valuable when early diagnosis is important. For this purpose, nucleic acid amplification (PCR, nucleic acid sequence-based amplification, self-sustained sequence replication, strand displacement amplification) is one of the most valuable tools not only for the diagnosis of infectious diseases but also used in advanced level research. The Loop-Mediated Isothermal Amplification (LAMP) is a unique nucleic acid amplification technique for diagnosis of various pathogens introduced at 2000 by Notomi and his colleagues which is simple, easy, rapid and cost effective when compared to PCR due to its high specificity, sensitivity, and rapidity. It uses a set of six primers and a DNA polymerase with stranddisplacement activity. Major advantage of LAMP method is its cost-effectiveness as it can be done simply by using waterbath or heating block. Bangladesh J Med Microbiol 2017; 11 (2): 20-27

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Section: Resultsmentioning

confidence: 99%

Section: Detection Of Plant Virusesmentioning

confidence: 99%

Section: Detection Of Animal Virusesmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification (LAMP) for Detection of Various Organisms: A Review

Akram¹

2017

Bangladesh J Med Microbiol

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“…In addition to detection of viral diseases [50], microbial infections have utilized LAMP-based detection, successfully, and often directly from clinical samples without isolating RNA [18,51]. While directly using patient samples, a single set of specific primers targeted toward the fusion-protein gene could produce amplicons in two hours.…”

Section: Lamp For the Diagnosis Of Viral Diseasesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Augustine

Hasan

Das

et al. 2020

Biology

200

166

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The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.

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“…As COVID-19 became a serious emerging epidemiological threat, an efficient clinical diagnostic tool has become an urgent need to reduce the contagiousness of the disease, to understand the virus better, and to be able to detect or even inhibit the infection in its early stages, hence, obtain a better prognosis and therapeutic outcomes. Several diagnostic tools have been used to detect different types of viruses, including the revers-transcriptase polymerase chain reaction (RT-PCR), which is used to detect the presence of viral genetic material from the isolated patient samples [11]; serological assay such as enzyme-linked immunosorbent assay (ELISA), which depends on the antibodies (i.e., IgM and IgG) produced naturally by the human defense system and found in the blood or saliva samples and which can be used as detection for the virus [12]; and Loop-Mediated isothermal amplification (LAMP) assay [13]. However, these diagnostic tests have many limitations and drawbacks such as time-consuming, high cost, low sensitivity, and the need for specialists.…”

Section: Introductionmentioning

confidence: 99%

Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles

et al. 2021

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Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as ‘surface plasmonic resonance’ in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients’ swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (−30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients’ swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention.

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Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Cited by 15 publications

References 157 publications

Loop-Mediated Isothermal Amplification (LAMP) for Detection of Various Organisms: A Review

Loop-Mediated Isothermal Amplification (LAMP) for Detection of Various Organisms: A Review

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles

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