Rapid Detection System for Hepatitis B Surface Antigen (HBsAg) Based on Immunomagnetic Separation, Multi-Angle Dynamic Light Scattering and Support Vector Machine

Abstract

Hepatitis B virus (HBV) is a significant public health problem worldwide. Hepatitis B surface antigen (HBsAg) is the principle marker for laboratory testing of HBV, but the rapid identification of HBsAg is challenging in a resource-limited setting. Antibodies to HBsAg (Anti-HBs) levels are measured as markers for an immune response to vaccination as well as for decision making for specific treatment against Hepatitis-B. This research developed a prototype for the rapid detection of HBsAg using immunomagnetic separation, dynamic light scattering, and support vector machine. Magnetic beads coated with polyclonal anti-HBsAg were used to isolate HBsAg from the sample. The performance characteristics of quantitative real-time detection of HBsAg were characterized under optimized conditions. Twelve photodetectors were arranged on four concentric curvatures at different angles. The photodetectors were positioned around the sample flask in forward direction. The prototype acquires the real-time laser scattering light from the sample, and the noise was removed. The power spectral features were extracted from the acquired signal. Support vector machines (SVM) were used for training a classification algorithm by using extracted features. The overall classification accuracy for the identification of HBsAg was 87.7%. The HBsAg detection test was also performed on 20 serum specimens, with 10 serum samples were positive for HBsAg and 10 were healthy control subjects. The test had a dynamic range of 98.86 IU/mL to 3163.5 IU/mL. Results of HBsAg detection agreed completely with those of conventional Chemiluminescence Immunoassay (CLIA). In conclusion, the proposed HBsAg detection method can differentiate the sample that contains HBsAg enriched IM beads and blank IM beads.

Recent Progress in Spectroscopic Methods for the Detection of Foodborne Pathogenic Bacteria

“…The designed prototype was further extended and applied for the detection of hepatitis B surface antigen (HBsAg) based on immunomagnetic separation. The results showed classification accuracy for the identification of HBsAg was 87.7%, with a dynamic range of 98.86 IU/mL to 3163.5 IU/mL [ 95 ].…”

Dengue virus infection: A review of advances in the emerging rapid detection methods

“…Recently the increase in dengue virus infection among various countries has led to developing a rapid and miniaturized point-of-care (POC) test for dengue diagnosis (Jain et al, 2021). The POC tests are essential, especially in remote areas with limited resource settings, where the highly cost diagnostic tests are not possible to perform (Hussain et al, 2020a;Hussain et al, 2020b;Hussain et al, 2020c) (Eivazzadeh-Keihan et al, 2020. The development of dengue diagnostic tools is improving with the advancement in micro-and nanofabrication bioelectronic devices.…”

Washing-free Chemiluminescence Immunoassay for Rapid Detection of cTnI in Whole Blood Samples

“…Various analysis techniques for cTnI detection have been developed to improve precision and sensitivity, including enzyme-linked immunosorbent assays (ELISA) [8], chemiluminescence immunoassays (CLIA) [9], and lateral ow immunoassays (LFIA) [10,11]. With the rapid development of signal ampli cation strategy and magnetic nanoparticles technology, chemiluminescence immunoassay has become the most ideal method for the detection of hs-cTnI [9,[12][13][14].…”