Rapid differentiation between bacterial infections and cancer using a near-infrared fluorogenic probe

Wu, Lingling; Wang, Qinghua; Wang, Yali; Zhang, Na; Zhang, Qingyang; Hu, Hai‐Yu

doi:10.1039/d0sc00508h

Cited by 42 publications

(33 citation statements)

References 23 publications

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“…Recently, quite a few NTR‐activatable optical probes or theranostic systems have been reported. [ 38–49 ] The general working mechanisms are summarized in Figure 4.…”

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

“…A number of activatable NIR FL probes (e.g., 7 ‒ 9 ) have been successfully obtained by means of the nitro‐to‐amino transformation strategy, demonstrating the feasibility of this means. [ 44–49 ]…”

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

“…Compound 11 is a neomycin‐based NTR‐activatable fluorogenic probe, which is able to be used by Hu's group to specifically identify bacterial infection from cancer. [ 44 ] Probe 11 consists of a para ‐nitro aromatic group‐decorated cyanine 7 (Cy 7) and a carboxylic ester‐linked neomycin. The nitro moiety of the para ‐nitro phenyl sulfonic group in 11 was reduced to the amino group by NTR, giving rise to the light‐up of the NIR FL of the latent Cy7.…”

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

“…Reproduced with permission. [ 44 ] Copyright 2020, Royal Society of Chemistry. Activated (D) NIR‐II FL imaging, (E) 3D PA imaging, and (F) PTT by hypoxia of tumor in vivo after being intravenously injected with IR1048‐MZ ( 12 ).…”

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

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Most recent advances on enzyme‐activatable optical probes for bioimaging

Mei

Tian

2021

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Enzymes are essential biological elements that play vital roles in many key cellular events and physiological processes. The dysregulation of enzyme activity broadly occurs in a large number of diseases ranging from inflammation to neurodegenerative disorders to tumors. Molecular imaging allows accurate and noninvasive visualization of biological analytes/physiological processes of interest closely linked to human health at different levels. Among various imaging modalities, optical imaging stands out benefited from its high sensitivity, excellent spatiotemporal resolution, real‐time mode, and facile accessibility. Diverse optical probes specifically activatable by disease‐relevant enzymes have sprung up. In comparison to the “always‐on” counterparts, the “off‐on” imaging probes activated by enzymes hold great promise for precise diagnosis of diseases at early stage with high target‐to‐background ratio, dramatically improved specificity, and significantly enhanced sensitivity. Herein, the most recent advances in optical probes activatable by enzymes for biosensing and bioimaging are briefly reviewed emphasizing their molecular design, working mechanism, and biomedical applications. Besides, some important prospects and the current challenges to fully implement the potential of enzyme‐activatable probes for precise and efficient theranostics in life science are also pointed out to hopefully arouse new insights into the development of new generation of theranostics.

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“…Recently, quite a few NTR‐activatable optical probes or theranostic systems have been reported. [ 38–49 ] The general working mechanisms are summarized in Figure 4.…”

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

Section: Advances Of Enzyme‐activatable Optical Probesmentioning

confidence: 99%

See 2 more Smart Citations

Most recent advances on enzyme‐activatable optical probes for bioimaging

Mei

Tian

2021

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“…This is because the probe showed fluorescence in response to NTR activity, which was confirmed by treatment with dicoumarol, an NTR inhibitor (Kong et al, 2019) (Figure 10D). Hu et al developed a near-infrared (NIR) fluorescent probe 23 consisting of cyanine dye attached to a p-nitroaromatic group for a rapid response to NTR and neomycin as an aminoglycoside antibiotic for bacterial targeting (Figure 11A) (Wu et al, 2020). Owing to the electron-withdrawing characteristic of the nitro group, the original NIR fluorescence feature of the cyanine dye was quenched.…”

Section: Nitroreductase Detectionmentioning

confidence: 99%

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

et al. 2021

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Identification of bacterial strains is critical for the theranostics of bacterial infections and the development of antibiotics. Many organic fluorescent probes have been developed to overcome the limitations of conventional detection methods. These probes can detect bacteria with “off-on” fluorescence change, which enables the real-time imaging and quantitative analysis of bacteria in vitro and in vivo. In this review, we outline recent advances in the development of fluorescence-based dyes capable of detecting bacteria. Detection strategies are described, including specific interactions with bacterial cell wall components, bacterial and intracellular enzyme reactions, and peptidoglycan synthesis reactions. These include theranostic probes that allow simultaneous bacterial detection and photodynamic antimicrobial effects. Some examples of other miscellaneous detections in bacteria have also been described. In addition, this review demonstrates the validation of these fluorescent probes using a variety of biological models such as gram-negative and -positive bacteria, antibiotic-resistant bacteria, infected cancer cells, tumor-bearing, and infected mice. Prospects for future research are outlined by presenting the importance of effective in vitro and in vivo detection of bacteria and development of antimicrobial agents.

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Metal‐Organic Framework‐Based Nanoheater with Photo‐Triggered Cascade Effects for On‐Demand Suppression of Cellular Thermoresistance and Synergistic Cancer Therapy

Yuan-yuan

Yan

et al. 2022

Adv Healthcare Materials

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Nanomedicine with stable light-heat conversion and spatiotemporally controllable drug activation is crucial for the success of photothermal therapy (PTT). Herein, a metal-organic framework (MOF)-based nanoheater with light-triggered multi-responsiveness is engineered to in-situ and on-demand sensitize cancer cells to local hyperthermia. Well-dispersed platinum nanoparticles synthesized inside nanospaces of the MOF are employed as the near-infrared (NIR)-harvesting unit with stable and high light-heat conversion performance. A conformation switchable polymer shell is constructed as a secondary light-responding unit to gate the targeted activation of a molecular inhibitor against thermoresistance. By cascade transformation of light stimuli to downstream signals, the nanoheater enables inhibitor release to go with local heating at the same time restricted in lesion sites to maximize efficacy and minimize systemic toxicity. The efficient photothermal conversion and the blockage of cellular heat-protective pathways provide a dual-mode of action which selectively sensitizes cancer cells to hyperthermia in a spatiotemporally controlled manner. With NIR as the remote switch, the MOF-based nanosystem demonstrates localized and boosted PTT efficacy against cancer both in vitro and in vivo. These results present nanosized MOFs as tailorable and versatile platforms for synergistic and precise cancer therapy.

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Rapid differentiation between bacterial infections and cancer using a near-infrared fluorogenic probe

Abstract: A new nitroreductase-responsive near-infrared fluorogenic probe can specifically image live bacteria in mouse models and does not accumulate at sites of inflammation or tumor.

Cited by 42 publications

References 23 publications

Most recent advances on enzyme‐activatable optical probes for bioimaging

Most recent advances on enzyme‐activatable optical probes for bioimaging

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

Metal‐Organic Framework‐Based Nanoheater with Photo‐Triggered Cascade Effects for On‐Demand Suppression of Cellular Thermoresistance and Synergistic Cancer Therapy

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