Rapid Discrimination and Feature Extraction of Three Chamaecyparis Species by Static-HS/GC–MS

Abstract

This study aimed to develop a rapid and accurate analytical method for discriminating three Chamaecyparis species (C. formosensis, C. obtusa, and C. obtusa var. formosana) that could not be easily distinguished by volatile compounds. A total of 23 leaf samples from three species were analyzed by static-headspace (static-HS) coupled with gas chromatography-mass spectrometry (GC-MS). The static-HS procedure, whose experimental parameters were properly optimized, yielded a high Pearson correlation-based similarity between essential oil and VOC composition (r = 0.555-0.999). Thirty-six major constituents were identified; along with the results of cluster analysis (CA), a large variation in contents among the three different species was observed. Principal component analysis (PCA) methods illustrated graphically the relationships between characteristic components and tree species. It was clearly demonstrated that the static-HS-based procedure enhanced greatly the speed of precise analysis of chemical fingerprint in small sample amounts, thus providing a fast and reliable tool for the prediction of constituent characteristics in essential oil, and also offering good opportunities for studying the role of these feature compounds in chemotaxonomy or ecophysiology.

Estimation of Total Phenolic Compounds and Non-Targeted Volatile Metabolomics in Leaf Tissues of American Chestnut (<i>Castanea dentata</i>), Chinese Chestnut (<i>Castanea mollissima</i>) and the Backcross Breeding Generations

“…Analysis of volatile organic compounds from leaves, SPME and GC/MS, were made on the same date as the sampling. VOCs samples preparation procedure was based on Chang et al with modifications [34]. Fresh leaves were immediately chopped into small pieces, and 5 g samples were placed in 1 L glass jars (Environmental Sampling Supply, San Leandro, CA) and sealed with aluminum foil and parafilm.…”

Antifungal Potential and Chemical Composition of Chamaecyparis formosensis and Chamaecyparis obtusa var. formosana Essential Oils in Liquid and Vapor Phases Against 4 Fungal Species

“…formosana , and Cunninghamia konishii . C. formosensis , better known as Taiwan red cypress, grows in the central mountain range of Taiwan at elevations of 1500 to 2150 m. 2,3 Renowned for its excellent quality wood and attractive fragrance, C. formosensis has a thin bark of pale pinkish color. By contrast, C. obtusa var.…”

Optimization of a static headspace GC-MS method and its application in metabolic fingerprinting of the leaf volatiles of 42 citrus cultivars

“…As an efficient and convenient sampling technique ( González-Mas et al., 2009 ; Escriche et al., 2011 ; Jeleń et al., 2017 ), static headspace (static-HS) sampling combined with gas chromatography-mass spectrometry (GC-MS) analysis is an ideal method for analyzing plant VOCs ( Chen et al., 2015 ; Mohammadhosseini et al., 2017 ), facilitating the simple assay of a large number of sample matrices. In addition, compared with HS solid-phase microextraction (HS-SPME), it has the advantages of avoiding costly and time-consuming sample preparation and enables continuous sampling, as the extraction head needs not be replaced between samples ( Lorenzo et al., 2002 ; Yun et al., 2021 ).…”