Rapid electrochemical-biosensor microchip platform for determination of microalbuminuria in CKD patients

Tseng, Chin Chung; Ko, Chien Hsuan; Lu, Song Yu; Yang, Chia En; Fu, Lung-Ming; Li, Chi Yu

doi:10.1016/j.aca.2020.12.029

Cited by 18 publications

(11 citation statements)

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“…CKD is generally diagnosed through blood and urine tests. Human urine consists of around 95% water and a large number of smaller constituents, such as uric acid, ALB, CRE, sodium, potassium, ammonia, nitrogen, magnesium, and others [ 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. Urine may also include precipitates of calcium, phosphorus, and other compound crystals [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that paper-based devices provide a viable method for detecting early CKD by assessing the concentration of ALB, MAU (microalbuminuria), CRE, or ACR in urine samples [ 4 , 19 , 46 , 47 , 48 , 49 , 50 ]. For example, Cai et al [ 46 ] presented a field amplification paper-based device for sensitive colorimetric detection of MAU in human urine.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

et al. 2022

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A novel assay platform consisting of a microfluidic sliding double-track paper-based chip and a hand-held Raspberry Pi detection system is proposed for determining the albumin-to-creatine ratio (ACR) in human urine. It is a clinically important parameter and can be used for the early detection of related diseases, such as renal insufficiency. In the proposed method, the sliding layer of the microchip is applied and the sample diffuses through two parallel filtration channels to the reaction/detection areas of the microchip to complete the detection reaction, which is a simple method well suited for self-diagnosis of ACR index in human urine. The RGB (red, green, and blue) value intensity signals of the reaction complexes in these two reaction zones are analyzed by a Raspberry Pi computer to derive the ACR value (ALB and CRE concentrations). It is shown that the G + B value intensity signal is linearly related to the ALB and CRE concentrations with the correlation coefficients of R2 = 0.9919 and R2 = 0.9923, respectively. It is additionally shown that the ALB and CRE concentration results determined using the proposed method for 23 urine samples were collected from real suffering chronic kidney disease (CKD) patients are in fine agreement with those acquired operating a traditional high-reliability macroscale method. Overall, for point-of-care (POC) CKD diagnosis and monitoring in clinical applications, the results prove that the proposed method offers a convenient, real time, reliable, and low-spending solution for POC CKD diagnosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

et al. 2022

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“…Because of their excellent sensitivity, very low detection limit, and outstanding selectivity, electrochemical sensors are used for microalbumin detection. [4][5][6][7] Iron-based alloys and compounds have provided an excellent foundation for biosensing applications; iron-tin alloy-based sensors are capable of electrically detecting a eld, and are becoming increasingly important for sensing applications. 8 Improved biosensing elements are one eld of biosensor development.…”

Section: Introductionmentioning

confidence: 99%

A flexible mesoporous Cu doped FeSn–G–SiO₂composite based biosensor for microalbumin detection

Areerob

Chanthai

2022

RSC Adv.

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“…Generally speaking, the detection limit and resolution of the lab-on-paper platform depends on the choice of the detection method. Many different detection methods have been adopted, including the fluorescence method, colorimetric method, chemiluminescence (CL) method, electrochemical (EC) method, surface-enhanced Raman spectroscopy (SERS) method, electrochemiluminescence (ECL) method, spectrometry method, and distancebased method [29][30][31][32][33][34][35][36]. Among these methods, distance-based methods and colorimetric methods are the most convenient and do not need the use of any expensive detection apparatus.…”

Section: Introductionmentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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In recent years, microfluidic lab-on-paper devices have emerged as a rapid and low-cost alternative to traditional laboratory tests. Additionally, they were widely considered as a promising solution for point-of-care testing (POCT) at home or regions that lack medical infrastructure and resources. This review describes important advances in microfluidic lab-on-paper diagnostics for human health monitoring and disease diagnosis over the past five years. The review commenced by explaining the choice of paper, fabrication methods, and detection techniques to realize microfluidic lab-on-paper devices. Then, the sample pretreatment procedure used to improve the detection performance of lab-on-paper devices was introduced. Furthermore, an in-depth review of lab-on-paper devices for disease measurement based on an analysis of urine samples was presented. The review concludes with the potential challenges that the future development of commercial microfluidic lab-on-paper platforms for human disease detection would face.

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Rapid electrochemical-biosensor microchip platform for determination of microalbuminuria in CKD patients

Cited by 18 publications

References 58 publications

Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

A flexible mesoporous Cu doped FeSn–G–SiO₂composite based biosensor for microalbumin detection

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

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Rapid electrochemical-biosensor microchip platform for determination of microalbuminuria in CKD patients

Cited by 18 publications

References 58 publications

Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

Microfluidic Sliding Paper-Based Device for Point-of-Care Determination of Albumin-to-Creatine Ratio in Human Urine

A flexible mesoporous Cu doped FeSn–G–SiO2composite based biosensor for microalbumin detection

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

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A flexible mesoporous Cu doped FeSn–G–SiO₂composite based biosensor for microalbumin detection