Rapid Enumeration of Active Bacteria in Water After Disinfection

Lisle, John T.; Pyle, Barry H.; Broadaway, Susan C.; McFeters, Gordon A.

doi:10.4271/981761

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…Dans ces conditions, les cellules microbiennes peuvent adopter des changements physiologiques (réduction de l'activité métabolique) et morphologiques (réduction de la taille cellulaire) (KLANčNIK et al, 2009). Considéré comme une stratégie de survie à une carence en nutriments, cet état cellulaire chez les bactéries peut conférer une résistance aux désinfectants (LISLE et al, 1998;SABy et al, 1999). Par conséquent, les microorganismes présents dans l'eau disposent donc d'un éventail de stratégies leur permettant de survivre à un ou plusieurs des traitements mis en oeuvre pour la production d'eau potable.…”

Section: La Diversité Des Espèces Microbiennes Dans L'eau Potableunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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Section: La Diversité Des Espèces Microbiennes Dans L'eau Potableunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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“…All techniques discussed above can be applied to view biofilm on metal surfaces. The corrosion products can disturb microscopy observation whether after taking the sample from the corroded surfaces or in-situ observation [51][52][53]. The CLSM is better than other microscopy observation in this way [54].…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Some multidisciplinary techniques used in MIC studies

Wang

Wang²,

et al. 2006

Materials & Corrosion

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Long-Term Bacterial Dynamics in a Full-Scale Drinking Water Distribution System

et al. 2016

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Large seasonal variations in microbial drinking water quality can occur in distribution networks, but are often not taken into account when evaluating results from short-term water sampling campaigns. Temporal dynamics in bacterial community characteristics were investigated during a two-year drinking water monitoring campaign in a full-scale distribution system operating without detectable disinfectant residual. A total of 368 water samples were collected on a biweekly basis at the water treatment plant (WTP) effluent and at one fixed location in the drinking water distribution network (NET). The samples were analysed for heterotrophic plate counts (HPC), Aeromonas plate counts, adenosine-tri-phosphate (ATP) concentrations, and flow cytometric (FCM) total and intact cell counts (TCC, ICC), water temperature, pH, conductivity, total organic carbon (TOC) and assimilable organic carbon (AOC). Multivariate analysis of the large dataset was performed to explore correlative trends between microbial and environmental parameters. The WTP effluent displayed considerable seasonal variations in TCC (from 90 × 103 cells mL-1 in winter time up to 455 × 103 cells mL-1 in summer time) and in bacterial ATP concentrations (<1–3.6 ng L-1), which were congruent with water temperature variations. These fluctuations were not detected with HPC and Aeromonas counts. The water in the network was predominantly influenced by the characteristics of the WTP effluent. The increase in ICC between the WTP effluent and the network sampling location was small (34 × 103 cells mL-1 on average) compared to seasonal fluctuations in ICC in the WTP effluent. Interestingly, the extent of bacterial growth in the NET was inversely correlated to AOC concentrations in the WTP effluent (Pearson’s correlation factor r = -0.35), and positively correlated with water temperature (r = 0.49). Collecting a large dataset at high frequency over a two year period enabled the characterization of previously undocumented seasonal dynamics in the distribution network. Moreover, high-resolution FCM data enabled prediction of bacterial cell concentrations at specific water temperatures and time of year. The study highlights the need to systematically assess temporal fluctuations in parallel to spatial dynamics for individual drinking water distribution systems.

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Rapid Enumeration of Active Bacteria in Water After Disinfection

Cited by 4 publications

References 5 publications

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Some multidisciplinary techniques used in MIC studies

Long-Term Bacterial Dynamics in a Full-Scale Drinking Water Distribution System

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