2020
DOI: 10.1371/journal.pone.0237384
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Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR

Abstract: Dengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profile changed at the end of 2018. Indeed, the number of confirmed DENV-2 cases suddenly increased in October 2018 and DENV-2 became predominant at the country level in early 2019. We developed a Genotype Screening Proto… Show more

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Cited by 6 publications
(15 citation statements)
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“…Subsequently, the proportion of DENV-4 cases gradually increased among the samples tested by the arbovirus surveillance network in Vientiane Capital and became predominant in 2016 at the country level until October 2018. Since then, DENV-2 has overcome DENV-4 in Vientiane Capital and at the country level [33]. However, DENV-4 still circulated at a significant level in 2019 (13%; Figure 2).…”
Section: Discussionmentioning
confidence: 99%
“…Subsequently, the proportion of DENV-4 cases gradually increased among the samples tested by the arbovirus surveillance network in Vientiane Capital and became predominant in 2016 at the country level until October 2018. Since then, DENV-2 has overcome DENV-4 in Vientiane Capital and at the country level [33]. However, DENV-4 still circulated at a significant level in 2019 (13%; Figure 2).…”
Section: Discussionmentioning
confidence: 99%
“…For instance, in 2006, in Vientiane Capital 84.6% of the adults above 35 years (N = 1990) displayed IgG reactive to DENV antigen but only 9.4% of the children (≥6 months and <6 years; N = 1568) were found to be positive [26]. Since 2012, three major outbreaks have occurred in the Lao PDR predominated by DENV-3 (2012-13), DENV-4 (2016-18) and DENV-2 (2019-20) [17][18][19]. Even if DENV-1 was detected before 2010, among the samples tested in the Lao PDR [13,15,16], its circulation over the last 10 years was not actually very widespread in a national context.…”
Section: Discussionmentioning
confidence: 99%
“…Blood samples (5 mL of venous blood) were collected by clinicians from the arbovirus surveillance hospital network in Vientiane Capital and some of the Lao provinces [17][18][19]. Briefly, suspected patients, presenting dengue fever symptoms matching with the WHO's case definition (fever onset ≥38 • C for less than 7 days with at least one of the following accompanying symptoms: headache, myalgia, arthralgia, retro-orbital pain, digestive troubles or hemorrhaging), were included in this study after obtaining informed consent.…”
Section: Sample Collectionmentioning
confidence: 99%
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