Rapid high-performance liquid chromatographic assay for the simultaneous analysis of non-steroidal antiinflammatory drugs in plasma

Owen, Susan G.; Roberts, Michael S.; Friesen, W. T.

doi:10.1016/0378-4347(87)80512-1

Cited by 61 publications

(14 citation statements)

References 19 publications

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“…Iohexol clearance was calculated as described elsewhere. 21 Plasma concentrations of ketoprofen were measured with an HPLC, 22 with slight modifications. Determinations were made on 4 samples in parallel.…”

Section: Methodsmentioning

confidence: 99%

Early detection of ketoprofen-induced acute kidney injury in sheep as determined by evaluation of urinary enzyme activities

Raekallio

Saario-Paunio

Rajamäki³

et al. 2010

ajvr

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Objective-To evaluate early indicators of renal tissue destruction and changes in urinary enzyme activities in sheep during the first hours after acute kidney injury induced by administration of an overdose of an NSAID. Animals-12 adult female sheep. Procedures-Acute kidney injury was induced in 6 sheep by administration of ketoprofen (30 mg/kg, IV) and detected by evaluation of urinary protein concentration, iohexol clearance, and results of histologic examination. Six sheep served as control animals. Blood and urine samples were collected for up to 24 hours after administration of ketoprofen. Plasma concentrations of urea, creatinine, albumin, and total protein; plasma activities of alkaline phosphatase, acid phosphatase, γ-glutamyl transpeptidase (GGT), matrix metalloproteinase (MMP)-2, and MMP-9; and urinary creatinine and protein concentrations, specific gravity, and activities of alkaline phosphatase, acid phosphatase, GGT, lactate dehydrogenase, N-acetyl-β-D-glucosaminidase (NAG), MMP-2, and MMP-9 were measured. Urinary protein concentration and enzyme activities were normalized on the basis of urinary creatinine concentrations and reported as ratios. Results-Many urinary enzyme-to-creatinine ratios increased before the plasma creatinine concentration exceeded the reference value. Urine NAG, lactate dehydrogenase, and acid phosphatase activities were increased beginning at 2 hours after ketoprofen administration, and alkaline phosphatase, GGT, and MMP-2 activities were increased beginning at 4 hours after ketoprofen administration. Most peak urinary enzyme-to-creatinine ratios were detected earlier than were the highest plasma creatinine and urea concentrations. Conclusions and Clinical Relevance-Urinary enzyme activities were sensitive early indicators of acute kidney injury induced by an overdose of an NSAID in sheep. (Am J Vet

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Section: Methodsmentioning

confidence: 99%

Early detection of ketoprofen-induced acute kidney injury in sheep as determined by evaluation of urinary enzyme activities

Raekallio

Saario-Paunio

Rajamäki³

et al. 2010

ajvr

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“…A h.p.l.c. method was used to measure diclofenac, salicylate [13] and paracetamol [14]. The coefficients of variation for all solutes in the assay procedures were less than 5%.…”

Section: Methodsmentioning

confidence: 99%

Disappearance kinetics of solutes from synovial fluid after intra‐ articular injection.

Owen

Francis

Roberts

1994

Brit J Clinical Pharma

126

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“…The mobile phase was filtered through 0.45 µm nylon membrane filter and degassed prior to use. The mobile phase used by Owen et al (1987) was a mixture of 0.03% phosphoric acid (pH 2.5):acetonitrile at a ratio of 55:45. Samples were placed in the auto-sampler.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…Because it is expected that piroxicam quantity diffused into the receptor cell is very low. The method published by Owen et al (1987) was adapted and modified to suit the above requirements. The modification was carried out on the mobile phase as the mobile phase used by the authors was not able to separate the drug peak from the interference of the formulation excipients.…”

Section: Introductionmentioning

confidence: 99%

Modification and Validation of an HPLC Method for Quantification of Piroxicam.

Abdulkarim¹,

Abdullah²,

Sakeena³

et al. 2011

Int J Drug Del

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Piroxicam is a NSAID that is widely used in the treatment of joint pain and osteoarthritis. The objectives of the study were to modify and validate HPLC method so as to obtain an accurate, sensitive and precise method to quantify piroxicam concentrations without interference from the other ingredients presence in the formulation. The method published by Owen et al. was adapted and modified to suit the above requirements. The modification was carried out on the mobile phase as the mobile phase used by the authors was not able to separate the drug peak from the interference of the formulation excipients. The modified mobile phase consisted of 5 mM of disodium hydrogen phosphate adjusted to pH 3 with concentrated ortho phosphoric acid, methanol, acetonitrile and glacial acetic acid at ratios of 27:20:52:1 respectively. The method was validated and found to be specific, precise, accurate and reproducible even when run at different times of the same day or on different times on different days. The limit of detection and quantification were determined to be 0.035 µg/ml and 0.0625 µg/ml respectively. It could be concluded that this method could be used to determine piroxicam concentration in the samples collected from in vitro study of permeability through the synthetic membrane and excised rat skin.

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Rapid high-performance liquid chromatographic assay for the simultaneous analysis of non-steroidal antiinflammatory drugs in plasma

Cited by 61 publications

References 19 publications

Early detection of ketoprofen-induced acute kidney injury in sheep as determined by evaluation of urinary enzyme activities

Early detection of ketoprofen-induced acute kidney injury in sheep as determined by evaluation of urinary enzyme activities

Disappearance kinetics of solutes from synovial fluid after intra‐ articular injection.

Modification and Validation of an HPLC Method for Quantification of Piroxicam.

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