Rapid high-performance liquid chromatographic methods that screen for aromatic compounds in environmental samples

Krahn, Margaret M.; Ylitalo, Gina M.; Buzitis, Jon; Chan, Sin-Lam; Varanasi, Usha

doi:10.1016/0021-9673(93)80073-h

Cited by 39 publications

(19 citation statements)

References 30 publications

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“…3A) that were similar to those observed in bile of Atlantic salmon exposed to Monterey crude oil (Fig. 3B), as well as the patterns of bile from harbor seals that were visibly oiled by the Exxon Valdez oil spill (Krahn et al 1993). Although non-oiled turtles had measurable levels of PAH metabolites in their bile, their HPLC-F chromatographic patterns (Fig.…”

Section: Pah Metabolites In Bilesupporting

confidence: 72%

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Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Ylitalo¹,

Collier²,

Anulacion³

et al. 2017

Endang. Species. Res.

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Section: Pah Metabolites In Bilesupporting

confidence: 72%

“…Chromatographic HPLC-F patterns were examined to suggest a possible source of PAH exposure (e.g. pyrogenic or petrogenic chemical contaminants) (Krahn et al 1993). Visibly oiled sea turtles had chromatographic patterns (example of pattern shown in Fig.…”

Section: Pah Metabolites In Bilementioning

confidence: 99%

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Ylitalo¹,

Collier²,

Anulacion³

et al. 2017

Endang. Species. Res.

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“…Knowing the relative level of PAH exposure is important in understanding the link between PAH exposure, accumulation in edible tissues and resulting sublethal effects measured, particularly since PAHs exert their toxic effects after metabolism. 22,46,47 In addition, this study provides ample baseline data, which can be used to measure future environmental impacts in the GoM.…”

Section: ■ Introductionmentioning

confidence: 99%

PAH Exposure in Gulf of Mexico Demersal Fishes, Post-Deepwater Horizon

Snyder

Pulster

Wetzel

et al. 2015

Environ. Sci. Technol.

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Following the 2010 Deepwater Horizon (DWH) blowout, we surveyed offshore demersal fishes in the northern Gulf of Mexico (GoM) in 2011-2013, to assess polycyclic aromatic hydrocarbon (PAH) exposure. Biliary PAH metabolites were estimated in 271 samples of golden tilefish (Lopholatilus chamaeleonticeps), king snake eel (Ophichthus rex), and red snapper (Lutjanus campechanus), using high performance liquid chromatography with fluorescence detection. Mean concentration of naphthalene metabolites in golden tilefish (240 μg g(-1)) was significantly higher (p = 0.001) than in red snapper (61 μg g(-1)) or king snake eel (38 μg g(-1)). Biliary naphthalene metabolite concentration decreased over the study period in red snapper (58%) and king snake eel (37%), indicating likely episodic exposure, while concentrations were persistently high in golden tilefish. Naphthalene metabolite levels measured in golden tilefish are among the highest concentrations measured in fishes globally, while concentrations for red snapper and king snake eel are similar to pre-DWH levels measured in GoM species. In contrast, concentrations of benzo[a]pyrene metabolites were similar for all three species (p = 0.265, mean 220 ng g(-1)) and relatively low when compared to GoM, global data and previous oil spills. These data support previous findings that fish life history and physiology play significant roles in exposure and uptake of PAH pollution.

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“…Samples were analyzed for organic chemical contaminants as described by Krahn et al (1988) and Sloan et al (1993). Concentrations of organic chemicals in the samples from long-term exposure were analyzed using a validated screening technique utilizing highpressure liquid chromotography (HPLC) (Krahn et al, 1993(Krahn et al, , 1994. Toxic and essential elements in tissues from the field studies were determined as described in Robisch and Clark (1993).…”

Section: Tissue Chemistrymentioning

confidence: 99%

Environmental Contaminants and the Prevalence of Hemic Neoplasia (Leukemia) in the Common Mussel (Mytilus edulisComplex) from Puget Sound, Washington, U.S.A.

Krishnakumar

Casillas

Snider

et al. 1999

Journal of Invertebrate Pathology

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The relationship between hemic neoplasia, a blood cell disorder in bivalve molluscs, and chemical contaminants was evaluated in the common mussel (Mytilus edulis complex). Hemic neoplasia (HN) is endemic to mussel populations in Puget Sound. The prevalence of hemic neoplasia ranged from 0 to 30% in mussels from nine sites in Puget Sound, Washington. Organic chemical contamination in sediment from these sites range from 0.1 to 64.0 ppm of polycyclic aromatic hydrocarbons (PAHs) and 0.07 to 0.50 ppm chlorinated hydrocarbons. No relationship between the body burden of environmental contaminants and the prevalence of HN in mussels was identified. To evaluate the shortterm ability of chemical contaminants to induce HN in mussels, mussels, from a site where mussels were previously determined to be HN free, were fed microencapsulated PAHs (composed of a mixture of phenanthrene, flouranthene, and benzo[a]pyrene) or PCBs (Aroclor 1254) and the prevalence of HN was assessed after 30 days of exposure. Although an apparent increase in HN prevalence (20 to 30%) was observed in all treatments groups except the untreated controls, no significant difference in the prevalence of HN was observed between the control group of mussels fed corn oil (vehicle) and mussels fed either PAHs or PCBs in corn oil. A long-term (180-day) exposure study was conducted to evaluate the influence of PAHs or PCBs in modulating the prevalence of HN in a mussel population already exhibiting a moderate HN prevalence. Mussels, from a site where mussels were previously determined to exhibit a background prevalence of HN, fed microencapsulated PAHs, PCBs, and corn oil (vehicle) over a long time period (180 days), revealed an apparent increased prevalence of HN (30 to 40%) above the low levels (20%) initially present. However, no significant difference in the prevalence of HN was observed between the control group of mussels fed corn oil (vehicle) and mussels fed either PAHs or PCBs in corn oil. Although chemical contaminants have been proposed as a modulating factor in the development and promotion of HN in bivalve molluscs from environmentally stressed and degraded habitats, we find no evidence that chemical contaminants induce or promote the development of HN in the mussel M. edulis complex. Academic Press

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Rapid high-performance liquid chromatographic methods that screen for aromatic compounds in environmental samples

Cited by 39 publications

References 30 publications

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

PAH Exposure in Gulf of Mexico Demersal Fishes, Post-Deepwater Horizon

Environmental Contaminants and the Prevalence of Hemic Neoplasia (Leukemia) in the Common Mussel (Mytilus edulisComplex) from Puget Sound, Washington, U.S.A.

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