Rapid, highly sensitive detection of herpes simplex virus-1 using multiple antigenic peptide-coated superparamagnetic beads

Abstract

A sensitive, rapid, and label free magnetic bead aggregation (MBA) assay has been developed that employs superparamagnetic (SPM) beads to capture, purify, and detect model proteins and the herpes simplex virus (HSV). The MBA assay is based on monitoring the aggregation state of a population of SPM beads using light scattering of individual aggregates. A biotin-streptavidin MBA assay had a femtomolar (fM) level sensitivity for analysis times less than 10 minutes, but the response of the assay becomes nonlinear at high analyte concentrations. A MBA assay for the detection of HSV-1 based on a novel peptide probe resulted in the selective detection of the virus at concentrations as low as 200 viral particles (vp) per mL in less than 30 min. We define the parameters that determine the sensitivity and response of the MBA assay, and the mechanism of enhanced sensitivity of the assay for HSV. The speed, relatively low cost, and ease of application of the MBA assay promise to make it useful for the identification of viral load in resource-limited and point-of-care settings where molecular diagnostics cannot be easily implemented.

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

“…The study reported a detection limit of 0.8 pM was achieved in a total sample volume of 0.09 mL. Further Ran et al [111] reported a rapid and sensitive, label free magnetic bead aggregation assay for the capture and detection of model proteins of HSV. The biotin streptavidin based magnetic bead assay had the sensitivity in the femtomolar range with an analysis time less than 10 minutes.…”

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

“…Nanosized superparamagnetic beads functionalized with specific surface are versatile materials used for various applications in chemical assay, biosensors, bioseparation systems, and biomedical studies (Rampini et al 2015;Rampini et al 2016;Ran et al 2014). To provide specificity, magnet beads can be decorated with ligands, such as antibodies, aptamers, and peptides, qualifying them as useful tools for the purification, detection, and separation of biological analytes from complex samples (Muzard et al 2012).…”

Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation

“…Similarly, Helms et al describe the generation of a multivalent peptide targeting collagen and a subsequent increase in binding affinity of >2 orders of magnitude compared to monovalent formats (Helms et al, 2009). Recently, our lab has described the capture of herpes simplex virus and anticancer proteins from soy whey, using multimeric peptidecoated magnetic particles Ran et al, 2014).…”

“…Recently, our lab has described the capture of herpes simplex virus and anticancer proteins from soy whey, using multimeric peptide-coated magnetic particles Ran et al 2014). …”