Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde

Douša, Michal; Břicháč, Jiří; Gibala, Petr; Lehnert, Petr

doi:10.1016/j.jpba.2010.11.026

Cited by 41 publications

(22 citation statements)

References 29 publications

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“…In the literature, it was shown that the application of postcolumn derivatization is more suitable due to simplicity and instability of the OPA fluorescent derivatives . The influence of flow rate of derivatization reagent , the derivatization reagent concentration , buffer pH , temperature of derivatization reaction , and finally the volume of capillary coil reactor on detector response have been investigated for the conditions of postcolumn derivatization.…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, it was shown that the application of postcolumn derivatization is more suitable due to simplicity and instability of the OPA fluorescent derivatives [3]. The influence of flow rate of derivatization reagent [3,4], the derivatization reagent concentration [5,6], buffer pH [3], temperature of derivatization reaction [5], and finally the volume of capillary coil reactor [3] on detector response have been investigated for the conditions of postcolumn derivatization.Analysis of volatile amines is difficult because of their high volatility, polarity, basic character, and lack of How to cite this article: Douša M, Doubský J. Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o-phthaldialdehyde/mercaptoethanol.…”

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confidence: 99%

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Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o‐phthaldialdehyde/mercaptoethanol

Douša

Doubský

2017

J of Separation Science

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The retention behavior of primary aliphatic amines (homologous series of aliphatic alkyl amines and cycloalkyl amines) and positional isomers of alkylamines in the hydrophilic interaction chromatography mode was studied. The study was carried out on a TSKgel Amide-80 column followed by postcolumn derivatization with fluorescence detection to describe the retention mechanism of tested compounds. The effect of chromatographic conditions including column temperature, acetonitrile content in the mobile phase, mobile phase pH (ranging from 3.5 to 6.8), and salt concentration in the mobile phase was investigated. The final mobile phase consisted of acetonitrile and solution of 20 mM potassium formate pH 3.5 in ratio 80:20 v/v. The analyses were carried out at mobile phase flow rate of 1.0 mL/min and the column temperature of 20°C. The developed method was fully validated in terms of linearity, sensitivity (limit of detection and limit of quantification), accuracy, and precision according to International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guidelines. The proposed new methods were proved to be highly sensitive, simple, and rapid, and were successfully applied to the determinations of isopropylamine, cyclohexylamine, and cyclopropylamine in relevant active pharmaceutical ingredients.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o‐phthaldialdehyde/mercaptoethanol

Douša

Doubský

2017

J of Separation Science

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“…Although HILIC is used in many practical applications both, in the isocratic and in the gradient elution mode, for the separations of polar compounds such as nucleosides [5], glycans [6,7], peptides [8], amino acids [9,10] and other compounds, the retention mechanism is still not fully understood [11,12]. The retention in HILIC is more complicated compared to the retention in RP system due to many types of interactions involved in the separation process.…”

Section: Introductionmentioning

confidence: 99%

Prediction of gradient retention data for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Vaňková

Česla

2017

Journal of Chromatography A

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In this work, we have investigated the predictive properties of mixed-mode retention model and oligomeric mixed-mode model, taking into account the contribution of monomeric units to the retention, in hydrophilic interaction liquid chromatography. The gradient retention times of native maltooligosaccharides and their fluorescent derivatives were predicted in the oligomeric series with number of monomeric glucose units in the range from two to seven. The maltooligosaccharides were separated on a packed column with carbamoyl-bonded silica stationary phase and 15 gradient profiles with different initial and final mobile phase composition were used with the gradient times 5; 7.5 and 10min. The predicted gradient retention times were compared for calculations based on isocratic retention data and gradient retention data, which provided better accuracy of the results. By comparing two different mobile phase additives, the more accurate retention times were predicted in mobile phases containing ammonium acetate. The acidic derivatives, prepared by reaction of an oligosaccharide with 2-aminobenzoic acid or 8-aminonaphthalene-1,3,6-trisulfonic acid, provided more accurate predictions of the retention data in comparison to native oligosaccharides or their neutral derivatives. The oligomeric mixed-mode model allowed prediction of gradient retention times using only one gradient profile, which significantly speeded-up the method development.

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“…However, the stability of final products (1‐alkyl‐2‐thioindoles) is limited due to the spontaneous rearrangements , and therefore is suitable for postcolumn derivatization. A postcolumn derivatization in combination with separation of native polar amines such as amino acids and amino alcohols in HILIC mode might represent a suitable alternative to a precolumn derivatization followed by RP‐HPLC .…”

Section: Introductionmentioning

confidence: 99%

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Douša

Pivoňková

Sýkora

2016

J of Separation Science

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A rapid procedure for the determination of memantine based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol was performed at excitation and emission wavelengths of 345 and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, derivatization reagent flow rate, and reagents concentration were studied due to steric hindrance of amino group of memantine. The derivatization reaction was applied for the hydrophilic interaction liquid chromatography method which was based on Cogent Silica-C stationary phase with a mobile phase consisting of a mixture of 10 mmol/L citric acid and 10 mmol/L o-phosphoric acid (pH 6.0) with acetonitrile using an isocratic composition of 2:8 v/v. The benefit of the reported approach consists in a simple sample pretreatment and a quick and sensitive hydrophilic interaction chromatography method. The developed method was validated in terms of linearity, accuracy, precision, and selectivity according to the International Conference on Harmonisation guidelines. The developed method was successfully applied for the analysis of commercial memantine tablets.

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Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde

Cited by 41 publications

References 29 publications

Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o‐phthaldialdehyde/mercaptoethanol

Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o‐phthaldialdehyde/mercaptoethanol

Prediction of gradient retention data for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

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