Rapid identification and quantitative analysis of malachite green in fish via SERS and 1D convolutional neural network

Zhang, Zhaoyi; Li, Hefu; Huang, Lili; Wang, Hongjun; Niu, Huijuan; Yang, Zhenshan; Wang, Minghong

doi:10.1016/j.saa.2024.124655

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

2024

DOI: 10.1016/j.saa.2024.124655

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Rapid identification and quantitative analysis of malachite green in fish via SERS and 1D convolutional neural network

Zhaoyi Zhang,

Hefu Li,

Lili Huang

et al.

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Cited by 2 publications

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Analyzing TVB-N in snakehead by Bayesian-optimized 1D-CNN using molecular vibrational spectroscopic techniques: Near-infrared and Raman spectroscopy

Ouyang,

Fan,

Chang

et al. 2025

Food Chemistry

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Analyzing TVB-N in snakehead by Bayesian-optimized 1D-CNN using molecular vibrational spectroscopic techniques: Near-infrared and Raman spectroscopy

Ouyang,

Fan,

Chang

et al. 2025

Food Chemistry

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A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green

Wu,

Xu,

et al. 2024

Front. Sustain. Food Syst.

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Malachite green (MG), a triphenylmethane dye, is used in the aquaculture industry as a disinfectant and insect repellent due to its potent bactericidal and pesticidal properties. However, its use poses potential environmental and health risks. This study analyzed and designed two haptens using computer simulation. Serum data confirmed the feasibility of introducing an arm at the dimethylamine group. Subsequently, a highly selective monoclonal antibody strain was successfully prepared based on the hapten. After optimizing the working conditions of indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), the IC50 value was 0.83 ng/mL, with a detection limit (IC10) of 0.08 ng/mL and a linear range of 0.19–3.52 ng/mL. The developed monoclonal antibody exhibited a crossover rate of less than 0.1% with other similar structures and can be used to establish an immunoassay.

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Rapid identification and quantitative analysis of malachite green in fish via SERS and 1D convolutional neural network

Cited by 2 publications

References 50 publications

Analyzing TVB-N in snakehead by Bayesian-optimized 1D-CNN using molecular vibrational spectroscopic techniques: Near-infrared and Raman spectroscopy

Analyzing TVB-N in snakehead by Bayesian-optimized 1D-CNN using molecular vibrational spectroscopic techniques: Near-infrared and Raman spectroscopy

A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green

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