Rapid identification by MALDI-TOF/MS and antimicrobial disk diffusion susceptibility testing for positive blood cultures after a short incubation on the WASPLab

Cherkaoui, Abdessalam; Renzi, Gesuèle; Azam, Nouria; Schorderet, Didier; Vuilleumier, Nicolas; Schrenzel, Jacques

doi:10.1007/s10096-020-03817-8

Cited by 14 publications

(12 citation statements)

References 25 publications

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“…It requires isolated colonies from overnight culture, saving only around 24 h compared to conventional AST ( Briggs et al, 2021 ). The incubation time, however, can be shortened down to 3–6 h incubation prior to MALDI-TOF MS ( Cherkaoui et al, 2020 ). Secondly, a MALDI-TOF MS system may cost up to €200,000 per year (excluding operational costs) rendering it impossible for use in outpatient clinics or small hospitals ( Vasala et al, 2020 ).…”

Section: Rapid Diagnostics Technologymentioning

confidence: 99%

Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy

2021

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Rising antibiotic resistance is a global threat that is projected to cause more deaths than all cancers combined by 2050. In this review, we set to summarize the current state of antibiotic resistance, and to give an overview of the emerging technologies aimed to escape the pre-antibiotic era recurrence. We conducted a comprehensive literature survey of >150 original research and review articles indexed in the Web of Science using “antimicrobial resistance,” “diagnostics,” “therapeutics,” “disinfection,” “nosocomial infections,” “ESKAPE pathogens” as key words. We discuss the impact of nosocomial infections on the spread of multi-drug resistant bacteria, give an overview over existing and developing strategies for faster diagnostics of infectious diseases, review current and novel approaches in therapy of infectious diseases, and finally discuss strategies for hospital disinfection to prevent MDR bacteria spread.

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Section: Rapid Diagnostics Technologymentioning

confidence: 99%

Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy

2021

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“…The availability of automated digital pictures of culture plates at defined time-points, as specified by the user, obviates the classical 24 h culture cycle. TLA had therefore an immediate and significant impact by enabling standardized and shorter durations of cultures (Dauwalder et al, 2016;Graham et al, 2016;Burckhardt et al, 2018Burckhardt et al, , 2019Cherkaoui et al, 2020a). Our laboratory implemented the WASPLab TM system in November 2018.…”

Section: Introductionmentioning

confidence: 99%

Impact of Total Laboratory Automation on Turnaround Times for Urine Cultures and Screening Specimens for MRSA, ESBL, and VRE Carriage: Retrospective Comparison With Manual Workflow

Cherkaoui

Renzi

Martischang

et al. 2020

Front. Cell. Infect. Microbiol.

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Using computerized time-stamps, we compared the turnaround-times (TAT) for urine samples and screening ESwabs of MRSA, VRE, and ESBL carriage in the bacteriology laboratory of Geneva University Hospitals between January and December 2017 (period preceding the implementation of the WASPLab TM) with the same specimen types analyzed between January and December 2019 (period after the implementation of the automation). During both 1-year periods, a total of 98'380 specimens were analyzed (48'158 in 2017 vs. 50'222 in 2019). On the WASPLab TM , all culture plates were imaged at defined intervals each day of incubation, but the processing of the cultures (i.e., pathogen identification and antimicrobial susceptibility testing) was only performed during day shift hours (∼8:00 A.M. to 4:30 P.M.). The median TAT for negative reports decreased by almost half for urine samples from 52.1 (2017) to 28.3 h (2019) (p < 0.001), and for MRSA screening specimens from 50.7 to 26.3 h (p < 0.001). The difference in median TAT for negative reports was less pronounced for screening of ESBL (50.2 vs. 43.0 h) (p < 0.001) and VRE (50.6 vs. 45.7 h) (p < 0.001). Despite a trend toward shorter result delivery for positive samples, there was no significant change in the median TAT. These results suggest that TAT for negative samples immediately benefit from automation, whereas TAT for positive samples also depend on the laboratory hours of operation and daily human resource management.

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“…The WASPLab ™ and Kiestra TLA are versatile technologies which can incorporate or can be combined with other diagnostic systems such as MALDI-TOF (Cherkaoui et al, 2011;Mutters et al, 2014), a key technique in modern medical microbiology to identify bacteria and fungi (Cherkaoui et al, 2010;Kaleta et al, 2011;Clark et al, 2013;Patel, 2019;Cherkaoui et al, 2020a). For example, the Kiestra TLA combined with MALDI-TOF has been shown to shorten the incubation time required to identify microbial pathogens (Mutters et al, 2014).…”

Section: Full Automation In Clinical Microbiology Laboratoriesmentioning

confidence: 99%

“…Since 2018, the Copan's WASPLab ™ technology has been implemented at the Geneva University Hospitals (Hopitaux Universitaires de Genève-HUG) (Cherkaoui et al, 2020c), where it has proven offering rapid detection of vancomycinresistant enterococci with automated incubation and digitalimage based analysis system (Cherkaoui et al, 2019b) and more generally, a substantial shortening of turn-around times (Cherkaoui et al, 2019a;Cherkaoui et al, 2020a).…”

Section: Full Automation In Clinical Microbiology Laboratoriesmentioning

confidence: 99%

“…The implementation of new technologies, like automation, ML and NGS, brings several issues. Automation of a clinical microbiology laboratory is challenging until it can reach all the steps, like opening all routinely used sample containers, relying on validated incubation times and standardized antibiotic susceptibility testing (Dauwalder et al, 2016;Cherkaoui et al, 2019a;Cherkaoui et al, 2020a;Vandenberg et al, 2020).…”

Section: Implementing New Technologies In Real-world Settings: Considmentioning

confidence: 99%

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Mini Review: Clinical Routine Microbiology in the Era of Automation and Digital Health

Leo

Cherkaoui

Renzi

et al. 2020

Front. Cell. Infect. Microbiol.

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Clinical microbiology laboratories are the first line to combat and handle infectious diseases and antibiotic resistance, including newly emerging ones. Although most clinical laboratories still rely on conventional methods, a cascade of technological changes, driven by digital imaging and high-throughput sequencing, will revolutionize the management of clinical diagnostics for direct detection of bacteria and swift antimicrobial susceptibility testing. Importantly, such technological advancements occur in the golden age of machine learning where computers are no longer acting passively in data mining, but once trained, can also help physicians in making decisions for diagnostics and optimal treatment administration. The further potential of physically integrating new technologies in an automation chain, combined to machine-learning-based software for data analyses, is seducing and would indeed lead to a faster management in infectious diseases. However, if, from one side, technological advancement would achieve a better performance than conventional methods, on the other side, this evolution challenges clinicians in terms of data interpretation and impacts the entire hospital personnel organization and management. In this mini review, we discuss such technological achievements offering practical examples of their operability but also their limitations and potential issues that their implementation could rise in clinical microbiology laboratories.

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Rapid identification by MALDI-TOF/MS and antimicrobial disk diffusion susceptibility testing for positive blood cultures after a short incubation on the WASPLab

Cited by 14 publications

References 25 publications

Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy

Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy

Impact of Total Laboratory Automation on Turnaround Times for Urine Cultures and Screening Specimens for MRSA, ESBL, and VRE Carriage: Retrospective Comparison With Manual Workflow

Mini Review: Clinical Routine Microbiology in the Era of Automation and Digital Health

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