Rapid in vivo screening method for the evaluation of new anti helicobacter medicinal preparations

Boda, Maurice; Tan, Paul V.; Nyasse, Barthélémy

doi:10.4314/ajtcam.v3i4.31182

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Taking into consideration these data as well as the limitations of in vitro efficacy experimentation, we also evaluated the in vivo eradication potency of HZJW in previously described rapid screening mouse model [20]. In the rapid urease assay, all animals in control group were negative for urease reaction, whereas in H .…”

Section: Resultsmentioning

confidence: 99%

Gastroprotective and anti-Helicobacter pylori potential of herbal formula HZJW: safety and efficacy assessment

Xie

Chen

Qing-he

et al. 2013

BMC Complement Altern Med

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BackgroundA traditional Chinese Medicine (TCM) formula, HZJW, has been applied in clinics in China for gastrointestinal disorders. However, the therapeutic mechanism underlying its efficacy and safety remained to be defined. The present investigation was undertaken to evaluate the formula HZJW for its gastroprotective potential, possible effect on Helicobacter pylori along with safety to justify its anti-ulcer action and safe clinical application.MethodsThe gastroduodenal cytoprotective potential was evaluated in rodent experimental models (HCl/Ethanol and NSAID-induced ulcer protocols). The anti-H. pylori property was assessed by agar dilution assay in vitro and analysis in vivo including rapid urease test, immunogold test and histopathology. For toxicity assessment, acute toxicity study was performed according to fixed dose procedure with a single oral administration of HZJW to mice. In the oral chronic toxicity, rats (80 males, 80 females) were administrated HZJW orally in 0, 1000, 2500, or 5000 mg/kg/day doses for 26 weeks (n = 40/group of each sex). Clinical signs, mortality, body weights, feed consumption, ophthalmology, hematology, serum biochemistry, gross findings, organ weights and histopathology were examined at the end of the 13- and 26-week dosing period, as well as after the 4-week recovery period.ResultsIn the HCl/Ethanol-induced ulcer model, it was observed that oral administration with HZJW (260, 520 and 1040 mg/kg) and ranitidine (250 mg/kg) significantly reduced the ulcerative lesion index (116.70 ± 36.4, 102.20 ± 18.20, 84.10 ± 12.1 and 73.70 ± 16.70) in a dose-dependent manner, respectively, with respect to control group (134.10 ± 31.69). Significant inhibition was also observed in ulcerative index from aspirin-induced ulcer model, with decreases of 35.40 ± 5.93, 31.30 ± 8.08, 26.80 ± 8.27and 20.40 ± 6.93 for the groups treated with HZJW and ranitidine, in parallel to controls (41.60 ± 10.80). On the other hand, treatment with HZJW efficaciously eradicated H. pylori in infected mice in rapid urease test (RUT) and immunogold antibody assay, as further confirmed by reduction of H. pylori presence in histopathological analysis. In the in vitro assay, MICs for HZJW and amoxicillin (positive control) were 125 and 0.12 μg/mL respectively. The LD50 of HZJW was over 18.0 g/kg for mice. No drug-induced abnormalities were found as clinical signs, body weight, food consumption, hematology, blood biochemistry, ophthalmology and histopathology results across three doses. No target organ was identified. The No Observed Adverse Effect Level (NOAEL) of HZJW was determined to be 5,000 mg/kg/day for both sexes, a dose that was equivalent to 50 times of human dose.ConclusionsThese results suggested the efficacy and safety of HZJW in healing peptic ulcer and combating H. pylori, which corroborated their conventional indications and contributed to their antiulcer pharmacological validation, lending more credence to its clinical application for the traditional treatment of stomach complaints symptomatic of peptic ...

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Section: Resultsmentioning

confidence: 99%

Gastroprotective and anti-Helicobacter pylori potential of herbal formula HZJW: safety and efficacy assessment

Xie

Chen

Qing-he

et al. 2013

BMC Complement Altern Med

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“…Phytochemical studies have shown that columbamine, jatrorrhizine, palmatine and menisperine are major diterpenoid lactones and alkaloids compounds in Radix Tinosporae [ 33 ]. Although no investigations on antibacterial activity of TSG, columbamine, jatrorrhizine and menisperine against H. pylori have been reported, there are a few reports on palmatine; 16 μg/mL palmatine in vitro or 50 mg/kg in vivo killed H. pylori [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo bactericidal activity of Tinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo and its main effective component, palmatine, against porcine Helicobacter pylori

Rong

Dong

et al. 2016

BMC Complement Altern Med

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BackgroundTinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo (TSG) is a traditional Chinese herb that has been used for the treatment of upper respiratory tract infection and has anti-bacterial and anti-ulcer activity. Our study investigated the bactericidal effects of TSG and its major component, palmatine, against a Helicobacter pylori (H. pylori) strain isolated from pig and the standard strain H. pylori SS1 in vitro and in vivo.MethodsH. pylori was isolated from pig and named H. pylori SCYA201401. For in vitro experiments, the inhibitory activity of TSG and palmatine against H. pylori SCYA201401 and H. pylori SS1 were tested by use of the agar cup diffusion technique. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined from the absence of H. pylori colonies on agar plates. Time-kill curves were used to evaluate bactericidal activity; the average number of colonies was calculated at 0 to 48 h after liquid incubation, with concentrations of drugs at 0.5, 1, and 2 × MIC. For in vivo experiments, H. pylori SCYA201401-infected mice were randomly divided into TSG, palmatine, triple therapy (omeprazole, clarithromycin, and amoxicillin), blank control, and model groups. The eradication ratios were determined by use of rapid urease tests and bacterial culture.ResultsIn vitro, the MIC and MBC of TSG against H. pylori SCYA201401 and SS1 were both 6250 μg/mL, whereas palmatine against H. pylori SCYA201401 was 6.25 μg/mL and against H. pylori SS1 was 3.12 μg/mL. The time-kill curves showed a dose-dependent, progressive decline in the numbers of viable bacteria up to 40 h. In vivo, the eradication ratios in the TSG and palmatine groups of mice were 80 and 50 % compared with 70 % in the triple-therapy group.ConclusionTSG and its major component, palmatine, have bactericidal activity against H. pylori in vitro and in vivo. The possibility that TSG or palmatine can be effective in the treatment of human and animals H. pylori infection deserves investigation.

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“…On the day following the last administration of the extracts the animals were fasted for 10-12 h, after which they were sacrificed. The stomachs were excised and gastric biopsy samples were taken and used to assess H. pylori clearance by Gram staining, rapid urease test, and culture (Fauchere, 1999;Boda et al, 2006).…”

Section: In Vivo Assessment Of Anti-h Pylori Activity In Infected Micementioning

confidence: 99%

In vitroandin vivoanti-Helicobacter/Campylobacteractivity of the aqueous extract ofEnantia chlorantha

Tan

Boda

Etoa

2010

Pharmaceutical Biology

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Rapid in vivo screening method for the evaluation of new anti helicobacter medicinal preparations

Cited by 4 publications

References 33 publications

Gastroprotective and anti-Helicobacter pylori potential of herbal formula HZJW: safety and efficacy assessment

Gastroprotective and anti-Helicobacter pylori potential of herbal formula HZJW: safety and efficacy assessment

In vitro and in vivo bactericidal activity of Tinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo and its main effective component, palmatine, against porcine Helicobacter pylori

In vitroandin vivoanti-Helicobacter/Campylobacteractivity of the aqueous extract ofEnantia chlorantha

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