Rapid method for detection of minimal bactericidal concentration of antibiotics

Bär, Werner; Bäde-Schumann, Ulrike; Krebs, Andreas; Cromme, Ludwig J.

doi:10.1016/j.mimet.2009.01.010

Cited by 23 publications

(18 citation statements)

References 24 publications

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“…Minimal bactericidal concentrations (MBCs) were measured using the method which has been adopted from National Committee for Clinical Laboratory Standards (NCCLS), with modifications 23 . In short, 50 μL peptide solutions were pipetted into microtiter plates at various concentrations ranging from 1 to 128 μM.…”

Section: Methodsmentioning

confidence: 99%

High specific selectivity and Membrane-Active Mechanism of the synthetic centrosymmetric α-helical peptides with Gly-Gly pairs

Wang

Chou

et al. 2015

Sci Rep

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We used a template-assisted approach to develop synthetic antimicrobial peptides, which differ from naturally occurring antimicrobial peptides that can compromise host natural defenses. Previous researches have demonstrated that symmetrical distribution patterns of amino acids contribute to the antimicrobial activity of natural peptides. However, there is little research describing such design ideas for synthetic α-helical peptides. Therefore, here, we established a centrosymmetric α-helical sequence template (y + hhh + y)n (h, hydrophobic amino acid; +, cationic amino acid; y, Gly or hydrophobic amino acid), which contributed to amphipathicity, and a series of centrosymmetric peptides was designed with pairs of small amino acids (Ala and Gly), which were utilized to modulate the biological activity. The centrosymmetric peptides with 3 repeat units exhibited strong antimicrobial activity; in particular, the Gly-rich centrosymmetric peptide GG3 showed stronger selectivity for gram-negative bacteria without hemolysis. Furthermore, beyond our expectation, fluorescence spectroscopy and electron microscopy analyses indicated that the GG3, which possessed poor α-helix conformation, dramatically exhibited marked membrane destruction via inducing bacterial membrane permeabilization, pore formation and disruption, even bound DNA to further exert antimicrobial activity. Collectively, the Gly-rich centrosymmetric peptide GG3 was an ideal candidate for commercialization as a clinical therapeutic to treat gram-negative bacterial infections.

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Section: Methodsmentioning

confidence: 99%

High specific selectivity and Membrane-Active Mechanism of the synthetic centrosymmetric α-helical peptides with Gly-Gly pairs

Wang

Chou

et al. 2015

Sci Rep

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“…This method has been adopted from NCCLS M26-A, 22 with modifications. 23,24 The Sd1 strains were adjusted to achieve a turbidity equivalent to a 0.5 McFarland (1 × 10 8 CFU/mL) and diluted (1:1000) 25 in brain heart infusion (Oxoid). A dilution series of the extract, ranging from 50 000 to 24 μg/mL, were prepared and then transferred to the broth in 14 tubes.…”

Section: Macrodilution Methodsmentioning

confidence: 99%

Effects ofOxalis barrelieriL. (Oxalidaceae) aqueous extract on diarrhea induced byShigella dysenteriaetype 1 in rats

Tagne

Noubissi

Fankem

et al. 2017

Health Science Reports

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Aim: Oxalis barrelieri is a medicinal plant commonly used in Cameroon, for the treatment of many diarrheal diseases. The antibacterial properties of O barrelieri aqueous extract (WOb) against Shigella dysenteriae type 1 were investigated in vitro and in vivo.Methods: Antibacterial activity was evaluated in vitro by disc diffusion method and by macrodilution method. S dysenteriae type 1 at a dose of 1.2 × 10 9 CFU was administrated orally to rats to induce shigellosis. For 6 consecutive days, diarrheic rats were treated with O barrelieri aqueous extract (50 and 100 mg/kg BW) or norfloxacin (20 mg/kg BW). The diarrheal stool weight and S dysenteriae type 1 density were assessed during the treatment period, and death rate recorded. Nitric oxide production in blood and in colonic homogenate and blood parameterswere assessed, and the histological section of the colon was performed in the survivors. Results:The minimal inhibitory concentration and minimal bactericidal concentration of WOb were, respectively, 6 mg/mL and 25 mg/mL. The mean minimal bactericidal concentration/minimal inhibitory concentration ratio for WOb against S dysenteriae type 1 was high (˃4); WOb could be classified as a bacteriostatic drug. WOb significantly (P < .01) reduced bacterial density and diarrheal stool weight. WOb decreased nitric oxide production (P < .01) in the large intestine and protected the mucosa of the colon from bacterial destruction. Conclusion:The results suggest that O barrelieri aqueous extract possesses bacteriostatic and antidiarrheal activities and reduces damages caused to intestinal mucosa barrier by pathogenic mechanisms of Shigella. This extract could be used as an alternative therapeutic for infectious diarrhea. KEYWORDSantibacterial susceptibility, antidiarrheal activity, Oxalis barrelieri, rat, Shigella dysenteriae type 1 | INTRODUCTIONIn mammals, the gastrointestinal tract harbors various microbes that play an essential role in maintaining its physiological homeostasis. ------------------------------------------------------------------This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…The MIC (minimum inhibitory concentration) is determined as the lowest dilution, which inhibited growth was measured by optical density at 600 nm wavelength using UV-visible spectrophotometry (SHIMADZU; with the length of light path as1 cm) and CFU (colony forming units) counting. In this part of study the noninoculated medium and inoculated EPO-free medium were considered as control samples also these tests were done in three repeats [24,25].…”

Section: Effect Of the Escitalopram Oxalate On Bacterial Growthmentioning

confidence: 99%

Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

2019

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Escitalopram oxalate (EO) is considered as one of the extensively prescribed antidepressant drug in Turkey and some other countries, therefore this research was aimed to study the interaction of the drug with DNA and study of the substance effect on bacterial growth. The absorption value of the drug solution at 238 nm was increased when DNA was added gradually to it and it showed hyperchromism effect. The value obtained for DNA binding constant (Kb) was 0.035 M −1. When we added the CuCl2 2H2O to the mixture, any breakage was not shown in double strand DNA in comparison with control DNA. In addition low concentration of EO couldn’t protect DNA (0.5273 µmole bp) against Hydroxyl free radical (0.12 µmole) although it could protect the DNA when it was at the same or higher concentrations (0.5273, 5.273 and 252.73 µmole) than the DNA concentration. In addition, MIC of the drug for E.coli and Bacillus subtilis was almost 0.185 mM and 0.55 mM respectively. The E.coli strain was killed at concentrations 45, 15, 5 mM while the Bacillus subtilis was stable against all of the concentrations.

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Rapid method for detection of minimal bactericidal concentration of antibiotics

Cited by 23 publications

References 24 publications

High specific selectivity and Membrane-Active Mechanism of the synthetic centrosymmetric α-helical peptides with Gly-Gly pairs

High specific selectivity and Membrane-Active Mechanism of the synthetic centrosymmetric α-helical peptides with Gly-Gly pairs

Effects ofOxalis barrelieriL. (Oxalidaceae) aqueous extract on diarrhea induced byShigella dysenteriaetype 1 in rats

Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

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