Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC in<i>Rheum rhaponticum</i>L. and<i>Rheum undulatum</i>L.

Smolarz, Helena D.; Hałka, Aneta; Chabros, O.; Dzido, Tadeusz H.

doi:10.1556/achrom.25.2013.1.7

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Some information on methodological aspects of isolation of the individual components, e.g. rhaponticin and desoxyrhaponticin are also available (Smolarz et al 2013a). For the first time, the composition and content of flavonoids was investigated by German scientists who discovered rutin in leaves of R. rhabarbarum (0.7%) and R. rhaponticum (0.61%) (Hörhammer and Müller 1954).…”

Section: Phytochemical Profile Of R Rhaponticum and R Rhabarbarummentioning

confidence: 99%

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Kołodziejczyk-Czepas

Liudvytska

2020

Phytochem Rev

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The Rheum genus (Polygonaceae) covers about 60 species of rhubarbs, including specimens with a long ethnomedicinal history in Asia, Europe and other regions of the world. The work reviews available literature (until March, 2020) on phytochemical profile, ethnomedicinal recommendations, biological activities, pharmacological uses and future prospects for therapeutic applications of Rheum rhabarbarum L. (garden rhubarb) and Rheum rhaponticum L. (rhapontic rhubarb). Although the above species are well-known vegetables, scientific interest in these plants is a relatively new issue; most of evidence of their biological activities and therapeutic potential derives from the last 15 years. Rhubarbs contain numerous bioactive substances, belonging to diverse groups of phytochemicals, e.g. stilbenes, anthraquinones and flavonoids. The registered special extract of R. rhaponticum (ERr731®) is administered to alleviate the menopause-related complaints. Furthermore, both ethnomedicinal surveys and recent studies on bioactive substances from rhubarbs indicate that these plants may have significantly broader range of beneficial effects such as antioxidant, anti-inflammatory, antimicrobial and cardioprotective activities.

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Section: Phytochemical Profile Of R Rhaponticum and R Rhabarbarummentioning

confidence: 99%

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Kołodziejczyk-Czepas

Liudvytska

2020

Phytochem Rev

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“…Its structural characteristics and chemical properties are summarized in a recent paper by Sun et al (2017). Quantitative analyses of rhaponticin content in plants include various advanced techniques such as ultraperformance liquid chromatography-diode array detection (UPLC-DAD), high-speed counter-current chromatography (HSCCC), b-cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC), zone electrophoresis with diode array detector (CZE-DAD), Fe 3 O 4 -based magnetic molecularly imprinted polymers (MMIPs) (Sun et al 2017) as well as thinlayer chromatography (TLC) (Smolarz et al 2013).…”

Section: Sources Of Rhaponticin and Its Content In Plantderived Prepamentioning

confidence: 99%

Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research

Kołodziejczyk-Czepas

Czepas

2019

Phytochem Rev

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Rhaponticin (3,3 0 ,5-trihydroxy-4 0methoxystilbene 3-O-b-D-glucoside; synonymrhapontin), is a stilbene compound, mainly found in various species of rhubarb (Rheum L.). The aglycone, rhapontigenin, is thought to be the biologically active form of rhaponticin. As a component of rhubarb, rhaponticin may be present in the human diet both in foods and dietary supplements. Although promising reports have been published on the various activities of rhaponticin (see: antioxidant, estrogenic, antithrombotic, anticancer and anti-inflammatory actions), no comprehensive review exists of its anti-inflammatory properties. Therefore, the aim of the present work is to summarize the existing evidence of the anti-inflammatory properties of rhaponticin and provide a prospective insight into research on this stilbenoid.

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Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecumL.) by HSCCC

Wang

Suo

et al. 2015

J Chromatogr Sci

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High efficiency and less solvent consumption are the essential requirements of high-speed countercurrent chromatography (HSCCC), especially for the large-scale preparation. In this study, an efficient HSCCC strategy with consecutive sample injection was successfully developed to rapidly separate and purify rhaponticin and rhapontigenin from the seeds of the Chinese medicinal herb fenugreek (Trigonella foenum-graecum L.). The effective separation was achieved using n-hexane-ethyl acetate-methanol-water (1:4:2:6, v/v/v/v) as the two-phase solvent system, in which the mobile phase was eluted at an optimized flow rate of 2.2 mL/min and a revolution speed of 850 rpm. After consecutively loading four identical fenugreek samples, each containing 120 mg, HSCCC separation yielded 146.4 mg of rhaponticin and 174.8 mg of rhapontigenin with purities of 98.6 and 99.1%, respectively, as determined by high-performance liquid chromatography at 320 nm. Their chemical structures were identified using UV spectroscopy, (1)H-NMR and (13)C-NMR. The HSCCC method with consecutive sample injection allowed faster separation and produced less solvent waste, suggesting that it is an efficient way to rapidly separate and purify natural products on a large scale.

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Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC inRheum rhaponticumL. andRheum undulatumL.

Cited by 3 publications

References 12 publications

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research

Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecumL.) by HSCCC

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