Rapid, modified oxidase test for oxidase-variable bacterial isolates

Tarrand, Jeffrey J.; Gröschel, D

doi:10.1128/jcm.16.4.772-774.1982

Cited by 159 publications

(68 citation statements)

References 5 publications

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“…The ability to assemble c-type cytochrome of A. tumefaciens strains was tested by the TMPD oxidase assay (Tarrand & Groschel, 1982). Positive oxidase test is observed by the presence of blue color within 10 s.…”

Section: Tetramethyl-p-phenylenediamine (Tmpd) Oxidase Testmentioning

confidence: 99%

Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochromecoxidase activity inAgrobacterium tumefaciens

Tanboon

Chuchue

Vattanaviboon

et al. 2009

FEMS Microbiology Letters

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Thioredoxin-like protein (TlpA) is a membrane-anchored periplasmic protein that contains a thioredoxin domain at its C-terminus. An Agrobacterium tumefaciens mutant lacking functional tlpA shows increased sensitivity to a superoxide generator, increased resistance to H2O2, and reduced cytochrome c oxidase activity. Whereas the levels of antioxidant enzymes, including total superoxide dismutase (SOD) and catalases, are unaltered, high expression of periplasmic SOD partially restores the superoxide hypersensitivity of the mutant, suggesting an accumulation of superoxide anions in the periplasm. The change in the ability of the mutant to cope with H2O2 stress is independent of OxyR, an H2O2 sensor and transcription regulator. The presented data indicate that TlpA not only is involved in the proper assembly of cytochrome c but is also implicated in the bacterial oxidative stress response.

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Section: Tetramethyl-p-phenylenediamine (Tmpd) Oxidase Testmentioning

confidence: 99%

Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochromecoxidase activity inAgrobacterium tumefaciens

Tanboon

Chuchue

Vattanaviboon

et al. 2009

FEMS Microbiology Letters

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“…The ability to assemble c-type cytochrome of A. tumefaciens strains was tested by the TMPD oxidase assay (Tarrand & Groschel, 1982). The test is based on the ability of TMPD to donate electrons specifically to cytochrome c that reduces cytochrome c oxidase, resulting in the production of a blue color from oxidized TMPD.…”

Section: B-galactosidase Assaymentioning

confidence: 99%

Mutation inscoaffects cytochromecassembly and alters oxidative stress resistance inAgrobacterium tumefaciens

Saenkham

Vattanaviboon

Mongkolsuk

2009

FEMS Microbiology Letters

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Sco (for the synthesis of cytochrome c oxidase) is a mitochondrial membrane protein essential for the correct assembly of cytochrome c oxidase. sco homolog genes exist in a wide variety of bacterial species. Inactivation of Agrobacterium tumefaciens sco leads to markedly decreased cytochrome c oxidase activity. This phenotype can be complemented by either supplementing the culture medium with copper or by a plasmid containing sco. The sco mutant also alters resistance to a superoxide generator menadione and H2O2. Mutational analysis of conserved cysteine residues and a histidine residue within the putative copper ions binding motif of Sco indicates that these residues are essential for the biological activity of Sco. To summarize, we find that A. tumefaciens sco is required for the delivery of copper into active cytochrome c oxidase and in maintaining optimal resistance levels to oxidative stress.

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“…Cell morphology and motility were observed under a phase-contrast microscope (Optiphot, Nikon; ×1000 magnification). Oxidase and catalase activities were investigated using 1 % tetramethyl-p-phenylenediamine [18] and 3 % H 2 O 2 , respectively. Growth was studied on R2A agar at different temperatures (4, 8, 15, 25, 30, 37 and 45 °C).…”

Section: Morphological Physiological and Biochemical Characterizationmentioning

confidence: 99%

Lysobacter profundi sp. nov., isolated from freshwater sediment and reclassification of Lysobacter panaciterrae as Luteimonas panaciterrae comb. nov.

Jin

Song

Sung

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic taxonomic study was carried out on strains CHu50b-3-2T and CHu40b-3-1 isolated from a 67 cm-long sediment core collected from the Daechung Reservoir at a water depth of 17 m, Daejeon, Republic of Korea. The cells of the strains were Gram-stain-negative, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of two strains with γ-Proteobacteria, which showed the highest pairwise sequence similarities to Lysobacter hankyongensis KTce-2T (96.5 %), Lysobacter pocheonensis Gsoil193T (96.3 %), Lysobacter ginsengisoli Gsoil 357T (96.1 %), Lysobacter solanacearum T20R-70T (96.1 %), Lysobacter brunescens KCTC 12130T (95.4 %) and Lysobacter capsici YC5194T (95.3 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a clear phylogenetic lineage with the genus Lysobacter . The major fatty acids were identified as summed feature 9 (iso-C17 : 1 ω9c and/or C18 : 1 10-methyl), iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. The respiratory quinone was identified as ubiquinone Q-8. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The genomic DNA G+C content was determined to be 66.8 mol% (genome) for strain CHu50b-3-2T and 66.4 mol% (HPLC) for strain CHu40b-3-1. Based on the combined genotypic and phenotypic data, we propose that strains CHu50b-3-2T and CHu40b-3-1 represent a novel species of the genus Lysobacter , for which the name Lysobacter profundi sp. nov. is proposed. The type strain is CHu50b-3-2T (=KCTC 72973T=CCTCC AB 2019129T). Besides Lysobacter panaciterrae Gsoil 068T formed a phylogenetic group together with strain Luteimonas aquatica RIB1-20T (EF626688) that is clearly separated from all other known Lysobacter strains. Based on the phylogenetic relationships together with fatty acid compositions, Lysobacter panaciterrae Gsoil 068T should be reclassified as a member of the genus Luteimonas: Luteimonas aquatica comb. nov. (type strain Gsoil 068T=KCTC 12601T=DSM 17927T).

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Rapid, modified oxidase test for oxidase-variable bacterial isolates

Cited by 159 publications

References 5 publications

Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochromecoxidase activity inAgrobacterium tumefaciens

Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochromecoxidase activity inAgrobacterium tumefaciens

Mutation inscoaffects cytochromecassembly and alters oxidative stress resistance inAgrobacterium tumefaciens

Lysobacter profundi sp. nov., isolated from freshwater sediment and reclassification of Lysobacter panaciterrae as Luteimonas panaciterrae comb. nov.

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