Rapid molecular identification of five species of Trichogramma occurring in the Canary Islands with notes on their distribution in banana groves

Pino, Modesto del; Rugman‐Jones, Paul F.; Hernández‐Suárez, Estrella; Polaszek, Andrew; Stouthamer, Richard

doi:10.1007/s10526-013-9519-x

Cited by 11 publications

(17 citation statements)

References 34 publications

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“…17 • 53 17" W). Emerging wasps were successfully identified by examination of morphological characters and molecular characterization by sequencing of the ITS2 gene [18,21]. T. achaeae was reared for at least 15 generations before starting the trials, using fresh ultraviolet-sterilized eggs of E. kuehniella as factious host in a climatic chamber (25 ± 1 • C, 60-80% RH and 16:8 h L:D photoperiod), according to the methodology proposed by Cabello et al [23].…”

Section: Insect Rearingmentioning

confidence: 99%

“…It is a generalist and arrhenotokous species originating from India, which parasitizes the eggs of 26 lepidopteran species belonging to 10 different families [19], but it currently has extensive worldwide distribution due to its possible accidental or voluntary dispersion [18]. This parasitoid has been purposefully introduced for the biological control of lepidopteran pests to various European, African, South and Central American countries [3,18] and has been recently recorded on Hawaii [20] and the Canary Islands [18,21]. In addition, T. achaeae is currently commercialized and used in some European and North African countries against different Lepidoptera species in more than 15 horticultural and ornamental crops [3,22].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Temperature on Life History and Parasitization Behavior of Trichogramma achaeae Nagaraja and Nagarkatti (Hym.: Trichogrammatidae)

Pino

Gallego

Suárez

et al. 2020

Insects

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Trichogramma achaeae Nagaraja and Nagarkatti (Hymenoptera: Trichogrammatidae) is currently used as biological control agent for several lepidopteran pests. Knowledge of thermal requirements is essential to optimize its rearing procedures and inundative releases. The biological characteristics and two-sex life table parameters of T. achaeae were determined at five constant temperatures (15, 20, 25, 30, and 35 °C) using Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs. T. achaeae was able to develop and survive from 15 °C to 30 °C, but not at 35 °C. Temperature significantly affected the preadult development time and adult longevity, decreasing when temperature increased from 15 °C to 30 °C. Temperature significantly altered the sex ratio, being female biased between 15 °C and 25 °C. Age-stage, two-sex life table analysis indicated that net reproductive rate (R0) was highest at 20 °C. Both the intrinsic rate of increase (r) and finite rate of increase (λ) increased with increasing temperature, while the mean generation time (T) decreased significantly. In addition, functional response of T. achaeae was studied, being significantly affected by temperature and host egg density, displaying a Holling type-I at 15 °C and a Holling type-II at 25 °C. The relevance of these results is discussed for the use of T. achaeae as biological control agent.

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Section: Insect Rearingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Temperature on Life History and Parasitization Behavior of Trichogramma achaeae Nagaraja and Nagarkatti (Hym.: Trichogrammatidae)

Pino

Gallego

Suárez

et al. 2020

Insects

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“…알벌과(Trichogrammatidae)(벌목: 좀 벌상과)에는 전 세계적으로 83속 620여종이 알려져 있으며 이 중 Trichogramma속 알기생벌은 주로 나비목 해충의 알에 기생 을 하는 중요한 생물적 방제 인자로 다양한 농작물과 산림에서 성공적으로 사용되고 있다 (Li, 1994;Pinto & Stouthamer, 1994;Smith, 1996;del Pino et al, 2013). 생물적 방제 수단으로 Trichogramma속 알기생벌을 이용하기 위한 가장 기본적이며 중요한 단계는 정확한 종 동정(species identification)이며 이를 통해 야외포장에서 대량방사가 성공할 수 있다 (Hassan, 1994).…”

Section: 알기생벌 천적은 숙주곤충의 알 부화를 막아 유충에 의한 피 해를 최소화 할 수 있으며 대량으로 증식이 가능하unclassified

“…지금까지 Trichogramma속 수컷의 생식기와 더듬이 등의 형태 적 특징 (Nagarkatti and Nagaraja, 1971)을 통해 약 200여 종이 알려졌으나 야외에서 암컷만 발견되는 경우와 종내 다양한 형태 적 변이, 불완전한 생식적 격리, 그리고 은폐종(cryptic species)으 로 인해 종 동정이 매우 복잡하고 어렵다 (Ciociola, 2001a;del Pino et al, 2013). 따라서 최근에는 핵내 리보솜 DNA 영역, 특히 5.8S와 28S 리보솜 RNA 사이에 있는 internal transcribed spacer (이하 ITS2로 표현)의 염기서열을 이용한 Trichogramma spp.…”

Section: 방제하고자 하는 해충에 대해 효과가 좋은 기생벌 선발이 중요 한데 대량사육 과정에서 목적하지 않은 종이 섞unclassified

“…Trichogramma 속 ITS2 영역의 DNA 염기서열을 해독한 결과 종내 변이가 적 고 종간 염기서열 전체길이와 변이 차이가 커 종 구별을 위해 사 용될 수 있었다 Li et al, 2004). 그동안 많은 연구자들에 의해서 Trichogramma속 종들의 ITS2 전체 염기서열이 밝혀지고 GenBank 데이터베이스에 등록되고 있 으며 형태적 분류가 어려운 은폐종을 구별하여 왔다 (Amornsak et al, 1998;Silva et al, 1999;Stouthamer et al, 1999;Ciociola et al, 2001a, b;Li et al, 2004;Dang et al, 2005;Davies et al, 2006;Honda et al, 2006;Gariepy et al, 2007;Li, 2007;Kumar et al, 2009;Sumer et al, 2009;Jeong et al, 2010;Sumer et al, 2011;Karimi et al, 2012;Poorjavad et al, 2012;del Pino et al, 2013). 그러나 형태적으로 구별이 매우 힘든 T. minutum과 T.…”

Section: 방제하고자 하는 해충에 대해 효과가 좋은 기생벌 선발이 중요 한데 대량사육 과정에서 목적하지 않은 종이 섞unclassified

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Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

Seo¹,

Jung²,

Park³

et al. 2014

Korean journal of applied entomology

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To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3′terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ≤0.005 and ≥0.080, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

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Taxonomy and Biological Control: New Challenges in an Old Relationship

et al. 2023

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Biological control and taxonomy are continuously developing fields with remarkable impacts on society. At least 80 years of literature have documented this relationship, which remains essentially the same in its mutualistic nature, as well as in its major challenges. From the perspective of Brazilian taxonomists, we discuss the impacts of important scientific and social developments that directly affect research in these areas, posing new challenges for this lasting relationship. The increasing restrictions and concerns regarding the international transit of organisms require improvements in research related to risk assessment for exotic biological control agents and also stimulate prospecting within the native biota. In our view, this is a positive situation that can foster a closer relationship between taxonomists and applied entomologists, as well as local surveys and taxonomic studies that are necessary before new programs and agents can be implemented. We discuss the essential role of molecular biology in this context, as an iconic example of the synergy between applied sciences and natural history. As our society comes to need safer and more sustainable solutions for food security and the biodiversity crisis, scientific progress will build upon this integration, where biological control and taxonomy play an essential role.

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Rapid molecular identification of five species of Trichogramma occurring in the Canary Islands with notes on their distribution in banana groves

Cited by 11 publications

References 34 publications

Effect of Temperature on Life History and Parasitization Behavior of Trichogramma achaeae Nagaraja and Nagarkatti (Hym.: Trichogrammatidae)

Effect of Temperature on Life History and Parasitization Behavior of Trichogramma achaeae Nagaraja and Nagarkatti (Hym.: Trichogrammatidae)

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

Taxonomy and Biological Control: New Challenges in an Old Relationship

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