Rapid multi-residue and multi-class qualitative screening for veterinary drugs in foods of animal origin by UHPLC-MS/MS

Abstract

Multi-class UHPLC-MS/MS was developed for the analysis of more than 160 regulated or banned compounds of various classes: anthelmintics including benzimidazoles, avermectins and others; antibiotics including amphenicols, beta-lactams, macrolides, pyrimidines, quinolones, sulphonamides and tetracyclines; beta-agonists; corticosteroids; ionophores; nitroimidazoles; non-steroidal anti-inflammatory agents; steroids; and tranquillisers. Samples were extracted with acetonitrile, without any additional purification step, and analysed by using UHPLC-MS/MS. Validation was done in accordance with the guidelines laid down by European Commission Decision 2002/657/EC for qualitative screening methods. This simple method proved applicable to routine screening for residues in egg, honey, milk and muscle samples at half the maximum concentration permitted by the European Union for each drug. In most cases, the target value was set at 5 µg kg(-1) for unauthorised compounds.

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

“…The same procedure or slight variations can be applied for the extraction of veterinary drugs resides from several matrices [53], increasing the scope of the analytical methods, which is an important factor for routine laboratories.…”

Reported prevalence and quantitative LC-MS methods for the analysis of veterinary drug residues in honey: a review

“…The coextractives can clog analytical columns and cause a prohibitive degree of signal interference. Techniques have been demonstrated partially to mitigate these complications including the use of guard columns, calibration using external in-matrix standards and internal standard addition (Gentili et al 2005;Stolker & Brinkman 2005;Kujawski & Namiesnik 2009;Robert et al 2013). Methods already used by enforcement agencies are given preferential consideration in this review and evaluated on validation, sensitivity and the breadth of amenable analytes.…”

Multiresidue method for the determination of pharmacologically active substances in egg and honey using a continuous solid-phase extraction system and gas chromatography–mass spectrometry

“…Alternative, less common sample preparation techniques for the determination of PASs in eggs include pressurized liquid extraction (Runnqvist et al, 2010), solid-phase microextraction (Huang, Zhang, & Feng, 2006) and matrix solid-phase dispersion (Garrido Frenich et al, 2010. A variety of chemically based analytical strategies for determining PASs in eggs and honey have recently been reported. Multimethods and, especially, multiclass methods have lately gained importance as aids to control PAS residues in food of animal origin (Gómez-Pérez et al, 2012;Robert et al, 2013). One of the most popular multiclass methods is based on gas chromatography with mass spectrometry detection (GC-MS), which provides a high sensitivity and specificity (Courant et al, 2007(Courant et al, , 2008Rejtharová & Rejthar, 2009;Sánchez-Brunete et al, 2005).…”