Rapid Multiplex Reverse Transcription-PCR Typing of Influenza A and B Virus, and Subtyping of Influenza A Virus into H1, 2, 3, 5, 7, 9, N1 (Human), N1 (Animal), N2, and N7, Including Typing of Novel Swine Origin Influenza A (H1N1) Virus, during the 2009 Outbreak in Milwaukee, Wisconsin

He, Jun; Bose, Michael E.; Beck, Eric T.; Fan, Jun; Tiwari, Sagarika; Metallo, Jacob A.; Jurgens, Lisa A.; Kehl, Sue C.; Ledeboer, Nathan A.; Kumar, Swati; Weisburg, William G.; Henrickson, Kelly J.

doi:10.1128/jcm.00998-09

Cited by 77 publications

(28 citation statements)

References 22 publications

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“…As a result, specimens with negative RIDTs are usually tested subsequently by more sensitive culture or molecular assays. PCR-based molecular assays have shown excellent clinical utility for the detection and identification of influenza viruses; numerous FDA-cleared commercial devices are now available (18,23,24).…”

mentioning

confidence: 99%

Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B

Roth²,

et al. 2014

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mentioning

confidence: 99%

Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B

Roth²,

et al. 2014

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“…Due to a seemingly constant increase in the number of outbreaks of highly pathogenic avian influenza (HPAI) caused by infections with subtype H5 or H7 viruses in many countries, RT-PCR and RT-qPCRs were especially designed for the broad detection and differentiation of these HA genes [18,[20][21][22] and their pathotypes on the basis of the HA cleavage site motif [23][24][25]. Often these assays are combined with a differentiation of the NA subtypes N1, N2, or N7 [26]. So far, only few studies have focused on the generic detection of the N9 gene [27] or the H7 gene [16,28].…”

Section: Discussionmentioning

confidence: 99%

Nucleic acid-based detection of influenza A virus subtypes H7 and N9 with a special emphasis on the avian H7N9 virus

et al. 2014

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In 2013, a novel influenza A virus of subtype H7N9 was transmitted from avian sources to humans in China, causing severe illness and substantial mortality. Rapid and sensitive diagnostic approaches are the basis of epidemiological studies and of utmost importance for the detection of infected humans and animals. We developed various quantitative reverse transcriptase PCR (RT-qPCR) assays for (i) the generic detection of the haemagglutinin (HA) gene of H7 viruses or the neuraminidase (NA) gene of N9 viruses, and (ii) the specific detection of HA and NA of the novel avian H7N9/2013 virus. The sensitivity of the newly developed assays was compared with previously published PCRs, and the specificity of all RT-qPCRs was examined using a panel of 42 different H7 and 16 different N9 isolates. Furthermore, we analysed the performance of the RT-qPCR assays with dilution series and diagnostic samples obtained from animal experiments. Our study provides a comprehensive set of RT-qPCR assays for the reliable detection of the novel avian H7N9 virus, with high sensitivity and improved and tailored specificity values compared with published assays. Finally, we also present data about the robustness of a duplex assay for the simultaneous detection of HA and NA of the avian influenza H7N9/2013 virus.

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“…For example, influenza A viral subtypes H1N1 H1N2, H2N1, H3N1, H3N2, and H2N3 are commonly associated with influenza infections in pigs. Other influenza A viral subtypes, including H9N2 and H5N1, are almost exclusively found in avian populations .…”

Section: Introductionmentioning

confidence: 99%

A One-Step RT-PCR Array for Detection and Differentiation of Zoonotic Influenza Viruses H5N1, H9N2, and H1N1

Yao

Liu

LiJuan

et al. 2013

J. Clin. Lab. Anal.

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The high sensitivity, rapidity, reproducibility, and specificity of this zoonotic influenza viruses rRT-PCR array has been verified as being sufficient to detect the presence of multiple zoonotic influenza viruses in a single assay. The zoonotic influenza viruses RT-PCR array might provide rapid identification of emergent zoonotic influenza viruses strains during influenza outbreaks and disease surveillance initiatives.

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Rapid Multiplex Reverse Transcription-PCR Typing of Influenza A and B Virus, and Subtyping of Influenza A Virus into H1, 2, 3, 5, 7, 9, N1 (Human), N1 (Animal), N2, and N7, Including Typing of Novel Swine Origin Influenza A (H1N1) Virus, during the 2009 Outbreak in Milwaukee, Wisconsin

Abstract: A large outbreak of novel influenza A (H1N1) virus (swine origin influenza virus [S-OIV]) infection in

Cited by 77 publications

References 22 publications

Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B

Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B

Nucleic acid-based detection of influenza A virus subtypes H7 and N9 with a special emphasis on the avian H7N9 virus

A One-Step RT-PCR Array for Detection and Differentiation of Zoonotic Influenza Viruses H5N1, H9N2, and H1N1

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