Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume

Barik, Durga Prasad; Naik, Soumendra K.; Mudgal, Anup K.; Chand, Pradeep K.

doi:10.1007/s11627-007-9040-y

Cited by 38 publications

(27 citation statements)

References 23 publications

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“…1d). The superiority of IBA over other auxins for in vitro rooting has been reported in Clitoria ternatea (Barik et al 2007;Mukhtar et al 2010;Singh and Tiwari 2012) …”

Section: In Vitro Rootingmentioning

confidence: 94%

“…TDZ has been successfully used in plant regeneration systems for many plant species (Faisal et al 2005;Ahmad et al 2006;Ahmad and Anis 2007a) including various legumes (Malik and Saxena 1992;Kanyand et al 1994;Sanago et al 1996;Eapen et al 1998;Victor et al 1999;Ahmad and Anis 2007b;Barik et al 2007). …”

Section: Effect Of Thidiazuronmentioning

confidence: 99%

“…Considering its importance and need for conservation, many reports on in vitro regeneration of Clitoria ternatea have come up lately (Lakshmanan and Dhanalakshmi 1990;Malabadi and Nataraja 2001;Rout 2005;Shahzad et al 2007;Barik et al 2007;Tiwari 2010, 2012;Mukhtar et al 2010;Mohamed and Taha 2011;Ismail et al 2011). In their studies, effect of only adenine based cytokinins viz.…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05-2.5 μM) was used as a supplement to the Murashige and Skoog's (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

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“…1d). The superiority of IBA over other auxins for in vitro rooting has been reported in Clitoria ternatea (Barik et al 2007;Mukhtar et al 2010;Singh and Tiwari 2012) …”

Section: In Vitro Rootingmentioning

confidence: 94%

Section: Effect Of Thidiazuronmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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“…For the induction of strong and stout root systems the elongated shoots were individually cultured on half strength MS medium with 1.0 mg/l IBA which gave the best response with 91.60 % of roots produced and on an average of 22.83 roots per shoot were recorded within 4 weeks. The effectiveness of IBA in rooting has been reported for many medicinal plants, viz; Aegle marmelos (Puhan and Rath 2012), Alpinia zerumbet (Rakkimuthu et al 2011), Clitoria ternatea L. (Barik et al 2007), Murraya koenigii (L.) Spreng (Bhuyan et al 1997) and Ocimum basilicum L. (Sahoo et al 1997). This is due to an imbalance between endogenous auxin and exogenously supplied auxin IBA (Manickam et al 2000).…”

Section: Discussionmentioning

confidence: 99%

In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant

Sharma

Kaur

Sharma

2014

Physiol Mol Biol Plants

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Micropropagation offers a great potential to produce millions of clonal individuals through tissue culture via induction of morphogenesis. The aim of this work was to obtain an efficient protocol for callus regeneration for Gentiana kurroo Royle. The morphogenic response of different explants (leaves, petioles, roots) varied and responded differently for regeneration according to combinations of growth regulators. The petiole explants were best responding for callus induction and subsequently for indirect and direct regeneration. The callus induction was achieved on MS basal + 1.0 mg/l benzyladenine (BA) and 3.00 mg/l naphthalene acetic acid (NAA). MS medium supplemented with 0.10 mg/l NAA and 1.0 mg/l thidiazuron (TDZ) was recorded as the best medium for indirect regeneration. However, for direct regeneration the maximum number of shoot emergence was observed on MS basal fortified with 0.10 mg/l NAA + 0.75 mg/l TDZ. Half strength MS basal supplemented with indole-3-butyric acid (IBA) 1.00 mg/l gave best response for root induction. Subsequently, the plantlets were transferred and 100 % survival rate was recorded only on autoclaved cocopeat. No morphological variations were recorded in the callus regenerated plantlets.

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“…Preservation of genetic stability in micropropagation of plants is of utmost importance. Genetically stable and true to type progenies could be obtained through axillary bud culture in vitro (Barik et al 2007). For any in vitro culture system, maintenance of genetic integrity of regenerated plantlets is of prime concern.…”

mentioning

confidence: 99%

In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction

Nair¹,

Reghunath²,

Soni³

et al. 2016

IJEAB

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Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume

Cited by 38 publications

References 23 publications

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant

In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction

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