Rapid Procedure for Estimating Cyclic Hydroxamate (DIMBOA) Concentration in Maize¹

Abstract

A rapid procedure was developed for estimating the concentration of a cyclic hydroxamic acid 2,4‐dihydroxy‐ 7‐methoxy‐l,4‐benzoxazin‐3‐one (DIMBOA) for potential use in a maize (Zea mays L.) breeding program. Samples (0.2 to 0.5 g stem tissue) were collected from seedlings at 36 cm to 41 cm extended leaf height. The rapid procedure, essentially a simplification of the colorimetric procedure of Hamilton, was significantly correlated with the latter (r = +0.82) based on the analysis of 13 inbred lines of maize.

The effect of hydoroxamic acid concentration at late growth stages of wheat on the performance of the aphid Sitobion avenae

“…However, these have concentrated on seedlings. Comparisons between analytical techniques should be made for flag leaves when the possibility of erroneous results using the non-specific ferric chloride method is increased due to the higher concentrations of phenols in mature plants (Long, Dunn & Routley, 1974;Leszczynski et al, 1989).…”

Transcriptional responses of Diabrotica virgifera virgifera larvae to benzoxazinoids

“…A simple colorimetric test (Long et al. ) was used to confirm the benzoxazinoid‐synthesis phenotype of each line. Ten plants from each line were germinated in moist vermiculite and allowed to grow for 7 days.…”

“…Plants of each line were grown in two separate greenhouses and self-pollinated to provide sufficient seed for experiments. A simple colorimetric test (Long et al 1974) was used to confirm the benzoxazinoid-synthesis phenotype of each line. Ten plants from each line were germinated in moist vermiculite and allowed to grow for 7 days.…”

Potencial alelopático de 2-benzoxazolinona (boa) e sua interação com atrazine no crescimento de plantas

“…Isolados de outras substâncias do tipo 1,4-benzoxazina (HM BOA-Glc ) têm sid o des critos em mil ho (Tipt on & Buell, 1967). Na planta intacta, BOA e MBOA ocorrem nas formas glucosídicas de onde os ácidos hidroxâmicos são enzimaticamente liberados após algum tipo de dano celular, como ataque de patógenos ou homogeinização (Long et al, 1974;8 Potencial alelopático de 2 -benzoxazolinona Venis & Watson, 1978). Virtanem & Hietala (1960), observaram que quando tecidos intactos são cuidadosamente extraídos, somente derivados glucosídicos são observados, indicando que não existe nenhum ácido hidroxâmico livre ou benzoxazolinona no tecido intacto vivo.…”